Gene description for SLC7A11
Gene name solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11
Gene symbol SLC7A11
Other names/aliases CCBR1
xCT
Species Homo sapiens
 Database cross references - SLC7A11
ExoCarta ExoCarta_23657
Vesiclepedia VP_23657
Entrez Gene 23657
HGNC 11059
MIM 607933
UniProt Q9UPY5  
 SLC7A11 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Endothelial cells 26027894    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Prostate cancer cells 25844599    
 Gene ontology annotations for SLC7A11
Molecular Function
    protein binding GO:0005515 IPI
    L-amino acid transmembrane transporter activity GO:0015179 IBA
    cystine:glutamate antiporter activity GO:0015327 IDA
    cystine:glutamate antiporter activity GO:0015327 RCA
    L-kynurenine transmembrane transporter activity GO:0140926 IDA
Biological Process
    amino acid transmembrane transport GO:0003333 IBA
    glutathione metabolic process GO:0006749 IEA
    visual learning GO:0008542 IEA
    response to toxic substance GO:0009636 IDA
    response to organic cyclic compound GO:0014070 IEA
    L-cystine transport GO:0015811 IDA
    L-glutamate transmembrane transport GO:0015813 IDA
    ventricular system development GO:0021591 IEA
    striatum development GO:0021756 IEA
    adult behavior GO:0030534 IEA
    regulation of neutrophil apoptotic process GO:0033029 IEA
    cellular response to oxidative stress GO:0034599 IEA
    glutathione transmembrane transport GO:0034775 IEA
    response to nicotine GO:0035094 IEA
    regulation of cell population proliferation GO:0042127 IEA
    regulation of melanin biosynthetic process GO:0048021 IEA
    lung alveolus development GO:0048286 IEA
    modulation of chemical synaptic transmission GO:0050804 IEA
    regulation of synapse organization GO:0050807 IEA
    regulation of protein transport GO:0051223 IEA
    response to redox state GO:0051775 IEA
    limb development GO:0060173 IEA
    lens fiber cell differentiation GO:0070306 IEA
    platelet aggregation GO:0070527 IEA
    intracellular glutamate homeostasis GO:0090461 IEA
    L-glutamate import across plasma membrane GO:0098712 IEA
    negative regulation of ferroptosis GO:0110076 IDA
    dipeptide import across plasma membrane GO:0140206 IEA
    L-kynurenine transmembrane transport GO:0140924 IDA
    regulation of cellular response to oxidative stress GO:1900407 IEA
    regulation of cysteine metabolic process GO:1901494 IEA
    regulation of glutathione biosynthetic process GO:1903786 IEA
    regulation of AMPA glutamate receptor clustering GO:1904717 IEA
    regulation of glutamate metabolic process GO:2000211 IEA
Subcellular Localization
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IPI
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 IDA
    membrane GO:0016020 TAS
    brush border membrane GO:0031526 IEA
    microvillus membrane GO:0031528 IEA
    apical part of cell GO:0045177 IEA
    astrocyte projection GO:0097449 IEA
 Experiment description of studies that identified SLC7A11 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 226
MISEV standards
EM
Biophysical techniques
GAPDH|CD9|FLOT1
Enriched markers
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
6
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 275
MISEV standards
EM
Biophysical techniques
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
AIF
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
 Protein-protein interactions for SLC7A11
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TERT  
Two-hybrid Homo sapiens
2 TNFAIP3 7128
Affinity Capture-MS Homo sapiens
3 SGCA  
Affinity Capture-MS Homo sapiens
4 KLRC2  
Affinity Capture-MS Homo sapiens
5 MAPK4  
Affinity Capture-MS Homo sapiens
6 KRTAP1-3  
Two-hybrid Homo sapiens
7 SCN3B  
Affinity Capture-MS Homo sapiens
8 MARCKS 4082
Proximity Label-MS Homo sapiens
9 ITLN1 55600
Affinity Capture-MS Homo sapiens
10 RAMP3  
Affinity Capture-MS Homo sapiens
11 TNFSF8  
Affinity Capture-MS Homo sapiens
12 HECTD3 79654
Affinity Capture-MS Homo sapiens
13 TMEM25  
Affinity Capture-MS Homo sapiens
14 LAMP1 3916
Proximity Label-MS Homo sapiens
15 CLEC2D  
Affinity Capture-MS Homo sapiens
16 HS2ST1 9653
Affinity Capture-MS Homo sapiens
17 TRIM26 7726
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
18 KRTAP1-1  
Two-hybrid Homo sapiens
19 KLRD1  
Affinity Capture-MS Homo sapiens
20 SFTPA2  
Affinity Capture-MS Homo sapiens
21 ARF6 382
Proximity Label-MS Homo sapiens
22 OTUB1 55611
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
23 SYVN1 84447
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
24 APOM 55937
Affinity Capture-MS Homo sapiens
25 TTC30B  
Affinity Capture-MS Homo sapiens
26 LYN 4067
Proximity Label-MS Homo sapiens
27 ADAM7  
Affinity Capture-MS Homo sapiens
28 MCAM 4162
Proximity Label-MS Homo sapiens
29 VSIG4  
Affinity Capture-MS Homo sapiens
30 PPTC7 160760
Affinity Capture-MS Homo sapiens
31 ARMC6 93436
Affinity Capture-MS Homo sapiens
32 KLRB1  
Affinity Capture-MS Homo sapiens
33 C12orf49  
Positive Genetic Homo sapiens
34 CLEC4E  
Affinity Capture-MS Homo sapiens
35 RAB2A 5862
Proximity Label-MS Homo sapiens
36 SREBF2 6721
Positive Genetic Homo sapiens
37 NAAA  
Affinity Capture-MS Homo sapiens
38 EPHA2 1969
Affinity Capture-MS Homo sapiens
39 KRAS 3845
Proximity Label-MS Homo sapiens
40 CD44 960
Affinity Capture-Western Homo sapiens
41 CAV1 857
Proximity Label-MS Homo sapiens
42 SLC3A2 6520
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
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