Gene description for APOM
Gene name apolipoprotein M
Gene symbol APOM
Other names/aliases G3a
HSPC336
NG20
apo-M
Species Homo sapiens
 Database cross references - APOM
ExoCarta ExoCarta_55937
Vesiclepedia VP_55937
Entrez Gene 55937
HGNC 13916
MIM 606907
UniProt O95445  
 APOM identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells 36408942    
Prostate cancer cells 25844599    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for APOM
Molecular Function
    lipid transporter activity GO:0005319 IBA
    lipid transporter activity GO:0005319 IDA
    phospholipid binding GO:0005543 IBA
    phospholipid binding GO:0005543 IDA
    antioxidant activity GO:0016209 IDA
Biological Process
    cholesterol efflux GO:0033344 IBA
    cholesterol efflux GO:0033344 IDA
    cholesterol efflux GO:0033344 IMP
    high-density lipoprotein particle remodeling GO:0034375 IBA
    high-density lipoprotein particle remodeling GO:0034375 IMP
    high-density lipoprotein particle assembly GO:0034380 IBA
    high-density lipoprotein particle assembly GO:0034380 ISS
    high-density lipoprotein particle clearance GO:0034384 IBA
    high-density lipoprotein particle clearance GO:0034384 ISS
    negative regulation of plasma lipoprotein oxidation GO:0034445 IDA
    lipoprotein metabolic process GO:0042157 IEA
    cholesterol homeostasis GO:0042632 IC
    reverse cholesterol transport GO:0043691 ISS
    cellular oxidant detoxification GO:0098869 IEA
Subcellular Localization
    extracellular region GO:0005576 TAS
    very-low-density lipoprotein particle GO:0034361 IBA
    very-low-density lipoprotein particle GO:0034361 IDA
    low-density lipoprotein particle GO:0034362 IBA
    low-density lipoprotein particle GO:0034362 IDA
    high-density lipoprotein particle GO:0034364 IBA
    high-density lipoprotein particle GO:0034364 IDA
    discoidal high-density lipoprotein particle GO:0034365 IDA
    spherical high-density lipoprotein particle GO:0034366 IDA
 Experiment description of studies that identified APOM in exosomes
1
Experiment ID 497
MISEV standards
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
EM
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 275
MISEV standards
EM
Biophysical techniques
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
AIF
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
5
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for APOM
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SEC11C 90701
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 DCBLD2 131566
Affinity Capture-MS Homo sapiens
3 HFE  
Affinity Capture-MS Homo sapiens
4 MR1  
Affinity Capture-MS Homo sapiens
5 GPX8 493869
Affinity Capture-MS Homo sapiens
6 ESYT2 57488
Affinity Capture-MS Homo sapiens
7 SLC7A11 23657
Affinity Capture-MS Homo sapiens
8 CLGN 1047
Affinity Capture-MS Homo sapiens
9 CNTNAP3  
Affinity Capture-MS Homo sapiens
10 SCNN1A  
Affinity Capture-MS Homo sapiens
11 CACNA2D2 9254
Affinity Capture-MS Homo sapiens
12 ENTPD6  
Affinity Capture-MS Homo sapiens
13 TCTN3  
Affinity Capture-MS Homo sapiens
14 PPM1B 5495
Affinity Capture-MS Homo sapiens
15 CLPTM1 1209
Affinity Capture-MS Homo sapiens
16 TUBB8 347688
Affinity Capture-MS Homo sapiens
17 HSPA13 6782
Affinity Capture-MS Homo sapiens
18 USP16  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 ADPGK 83440
Affinity Capture-MS Homo sapiens
20 ITGA6 3655
Affinity Capture-MS Homo sapiens
21 GGT7 2686
Affinity Capture-MS Homo sapiens
22 HGH1 51236
Two-hybrid Homo sapiens
23 CYB5D2  
Affinity Capture-MS Homo sapiens
24 TMEM67 91147
Affinity Capture-MS Homo sapiens
25 HS6ST1  
Affinity Capture-MS Homo sapiens
26 CNNM3 26505
Affinity Capture-MS Homo sapiens
27 CDYL 9425
Affinity Capture-MS Homo sapiens
28 ADAM21  
Affinity Capture-MS Homo sapiens
29 ITGA7 3679
Affinity Capture-MS Homo sapiens
30 B3GNT2 10678
Affinity Capture-MS Homo sapiens
31 CNTNAP3B  
Affinity Capture-MS Homo sapiens
32 CLSTN3 9746
Affinity Capture-MS Homo sapiens
33 ASIC1  
Affinity Capture-MS Homo sapiens
34 CT45A2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 KLF6  
Two-hybrid Homo sapiens
36 ADGRG6 57211
Affinity Capture-MS Homo sapiens
37 HLA-DQB1 3119
Affinity Capture-MS Homo sapiens
38 PLXNA3 55558
Affinity Capture-MS Homo sapiens
39 BDH1 622
Affinity Capture-MS Homo sapiens
40 SEMA4C 54910
Affinity Capture-MS Homo sapiens
41 KIAA0922  
Affinity Capture-MS Homo sapiens
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