Gene description for PPM1B
Gene name protein phosphatase, Mg2+/Mn2+ dependent, 1B
Gene symbol PPM1B
Other names/aliases PP2C-beta
PP2C-beta-X
PP2CB
PP2CBETA
PPC2BETAX
Species Homo sapiens
 Database cross references - PPM1B
ExoCarta ExoCarta_5495
Vesiclepedia VP_5495
Entrez Gene 5495
HGNC 9276
MIM 603770
UniProt O75688  
 PPM1B identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for PPM1B
Molecular Function
    magnesium ion binding GO:0000287 IEA
    protein serine/threonine phosphatase activity GO:0004722 IBA
    protein serine/threonine phosphatase activity GO:0004722 IDA
    protein binding GO:0005515 IPI
    myosin phosphatase activity GO:0017018 IEA
    manganese ion binding GO:0030145 IEA
    calmodulin-dependent protein phosphatase activity GO:0033192 IEA
    histone H2AXS140 phosphatase activity GO:0140791 IEA
    RNA polymerase II CTD heptapeptide repeat Y1 phosphatase activity GO:0180004 IEA
    RNA polymerase II CTD heptapeptide repeat T4 phosphatase activity GO:0180005 IEA
    RNA polymerase II CTD heptapeptide repeat S2 phosphatase activity GO:0180006 IEA
    RNA polymerase II CTD heptapeptide repeat S5 phosphatase activity GO:0180007 IEA
    RNA polymerase II CTD heptapeptide repeat S7 phosphatase activity GO:0180008 IEA
    MAP kinase serine/threonine phosphatase activity GO:1990439 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IEA
    protein dephosphorylation GO:0006470 IMP
    N-terminal protein myristoylation GO:0006499 ISS
    negative regulation of interferon-beta production GO:0032688 IMP
    peptidyl-threonine dephosphorylation GO:0035970 IDA
    regulation of canonical NF-kappaB signal transduction GO:0043122 IBA
    negative regulation of canonical NF-kappaB signal transduction GO:0043124 IMP
    negative regulation of defense response to virus GO:0050687 IMP
    positive regulation of canonical Wnt signaling pathway GO:0090263 IBA
    negative regulation of non-canonical NF-kappaB signal transduction GO:1901223 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleolus GO:0005730 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISS
    cytosol GO:0005829 TAS
    membrane GO:0016020 ISS
 Experiment description of studies that identified PPM1B in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PPM1B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 NEUROG1  
Affinity Capture-MS Homo sapiens
3 ROPN1  
Affinity Capture-MS Homo sapiens
4 RPSAP58 388524
Affinity Capture-MS Homo sapiens
5 HDAC4  
Affinity Capture-MS Homo sapiens
6 HIST2H2AB 317772
Affinity Capture-MS Homo sapiens
7 MAGEA3  
Affinity Capture-MS Homo sapiens
8 GJA1 2697
Affinity Capture-MS Homo sapiens
9 SKI 6497
Affinity Capture-MS Homo sapiens
10 YWHAE 7531
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
11 GAK 2580
Co-fractionation Homo sapiens
12 KARS 3735
Proximity Label-MS Homo sapiens
13 VHL  
Affinity Capture-MS Homo sapiens
14 PARK7 11315
Affinity Capture-MS Homo sapiens
15 ISG15 9636
Affinity Capture-MS Homo sapiens
16 KIAA1429 25962
Affinity Capture-MS Homo sapiens
17 C1orf87  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 VIL1 7429
Affinity Capture-MS Homo sapiens
19 PAK4 10298
Co-fractionation Homo sapiens
20 RAB40C 57799
Affinity Capture-MS Homo sapiens
21 CERKL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 PLK4  
Affinity Capture-MS Homo sapiens
23 MAP4K5 11183
Co-fractionation Homo sapiens
24 MAD2L2 10459
Two-hybrid Homo sapiens
25 KDR  
Two-hybrid Homo sapiens
26 HIST2H3PS2 440686
Affinity Capture-MS Homo sapiens
27 PPM1A 5494
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 PTK7 5754
Two-hybrid Homo sapiens
29 SNX27 81609
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 PKN2 5586
Co-fractionation Homo sapiens
31 CSNK1D 1453
Co-fractionation Homo sapiens
32 SOD1 6647
Affinity Capture-MS Homo sapiens
33 BPGM 669
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 PRNP 5621
Affinity Capture-MS Homo sapiens
35 TRIM59  
Affinity Capture-Western Homo sapiens
36 CASK 8573
Co-fractionation Homo sapiens
37 INSIG2  
Affinity Capture-MS Homo sapiens
38 S100A2 6273
Affinity Capture-MS Homo sapiens
39 USP12 219333
Affinity Capture-Western Homo sapiens
40 COX15 1355
Affinity Capture-MS Homo sapiens
41 ERBB3 2065
Two-hybrid Homo sapiens
42 HIST1H2AG 8969
Affinity Capture-MS Homo sapiens
43 PSMD14 10213
Affinity Capture-MS Homo sapiens
44 HIST1H3E 8353
Affinity Capture-MS Homo sapiens
45 VCP 7415
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 S1PR1 1901
Affinity Capture-MS Homo sapiens
47 IFI16 3428
Affinity Capture-MS Homo sapiens
48 S100A8 6279
Affinity Capture-MS Homo sapiens
49 PPP2CA 5515
Co-purification Homo sapiens
50 NOS2  
Affinity Capture-MS Homo sapiens
51 CSNK1E 1454
Co-fractionation Homo sapiens
52 MAGOH 4116
Affinity Capture-MS Homo sapiens
53 C9orf142  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 MAPK8 5599
Co-fractionation Homo sapiens
55 H2AFY 9555
Affinity Capture-MS Homo sapiens
56 FEZ2 9637
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 CYLD  
Affinity Capture-MS Homo sapiens
58 NR1I3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 COX4I1 1327
Affinity Capture-MS Homo sapiens
60 KDF1  
Affinity Capture-MS Homo sapiens
61 FBXL4 26235
Affinity Capture-MS Homo sapiens
62 ROR1 4919
Two-hybrid Homo sapiens
63 KIF15 56992
Proximity Label-MS Homo sapiens
64 XPO1 7514
Affinity Capture-MS Homo sapiens
65 IL25  
Affinity Capture-MS Homo sapiens
66 PPARG 5468
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
67 RASGRP1  
Affinity Capture-MS Homo sapiens
68 ANXA1 301
Affinity Capture-MS Homo sapiens
69 SIRPA 140885
Reconstituted Complex Homo sapiens
70 ERBB4 2066
Two-hybrid Homo sapiens
71 PGAM4 441531
Affinity Capture-MS Homo sapiens
72 EGFR 1956
Two-hybrid Homo sapiens
73 OSGEP 55644
Affinity Capture-MS Homo sapiens
74 FBXO6 26270
Affinity Capture-MS Homo sapiens
75 FAM63B 54629
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 LRRC32 2615
Affinity Capture-MS Homo sapiens
77 MAP3K7  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
78 HIP1R 9026
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 ECHS1 1892
Two-hybrid Homo sapiens
80 SPTBN1 6711
Affinity Capture-MS Homo sapiens
81 PRKCI 5584
Co-fractionation Homo sapiens
82 S100A7L2  
Affinity Capture-MS Homo sapiens
83 DDX53  
Co-fractionation Homo sapiens
84 ARMC6 93436
Affinity Capture-MS Homo sapiens
85 Smurf2  
Affinity Capture-Luminescence Mus musculus
86 SOAT1 6646
Affinity Capture-MS Homo sapiens
87 SCGN  
Affinity Capture-MS Homo sapiens
88 PRDM13 59336
Affinity Capture-MS Homo sapiens
89 UBL7  
Co-fractionation Homo sapiens
90 FNTB 2342
Affinity Capture-MS Homo sapiens
91 PVR 5817
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 DDRGK1 65992
Affinity Capture-MS Homo sapiens
93 VDAC1 7416
Affinity Capture-MS Homo sapiens
94 CHUK 1147
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
95 SUPT16H 11198
Affinity Capture-MS Homo sapiens
96 PABPC4 8761
Affinity Capture-MS Homo sapiens
97 NAA10 8260
Co-fractionation Homo sapiens
98 GSN 2934
Affinity Capture-MS Homo sapiens
99 KRT18 3875
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 ARRB2 409
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
101 MYL7  
Two-hybrid Homo sapiens
102 AATK  
Two-hybrid Homo sapiens
103 SPTAN1 6709
Affinity Capture-MS Homo sapiens
104 VAV2 7410
Two-hybrid Homo sapiens
105 TP53 7157
Affinity Capture-MS Homo sapiens
106 THUMPD3 25917
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
107 SSRP1 6749
Affinity Capture-MS Homo sapiens
108 USP35  
Affinity Capture-MS Homo sapiens
109 AURKB 9212
Affinity Capture-MS Homo sapiens
110 POLE3  
Affinity Capture-MS Homo sapiens
111 IKBKG 8517
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
112 RYK 6259
Two-hybrid Homo sapiens
113 ABL1 25
Co-fractionation Homo sapiens
114 CDK2 1017
Biochemical Activity Homo sapiens
Co-purification Homo sapiens
115 RASL11B  
Affinity Capture-MS Homo sapiens
116 EIF4A3 9775
Affinity Capture-MS Homo sapiens
117 APOM 55937
Affinity Capture-MS Homo sapiens
118 CDK1 983
Co-fractionation Homo sapiens
119 ROR2 4920
Two-hybrid Homo sapiens
120 TLE4  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
121 ESR1  
Affinity Capture-MS Homo sapiens
122 UFL1 23376
Affinity Capture-MS Homo sapiens
123 LRRK1 79705
Co-fractionation Homo sapiens
124 COQ2  
Affinity Capture-MS Homo sapiens
125 ACTA1 58
Affinity Capture-MS Homo sapiens
126 CDK13 8621
Co-fractionation Homo sapiens
127 SETD7 80854
Affinity Capture-MS Homo sapiens
128 STK40  
Co-fractionation Homo sapiens
129 ARRB1 408
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
130 HEATR5A 25938
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
131 LIPA 3988
Affinity Capture-MS Homo sapiens
132 LPAR2 9170
Affinity Capture-MS Homo sapiens
133 ANXA2 302
Affinity Capture-MS Homo sapiens
134 CDK6 1021
Biochemical Activity Homo sapiens
Co-purification Homo sapiens
135 VIM 7431
Affinity Capture-MS Homo sapiens
136 IKBKB 3551
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
137 PRMT1 3276
Affinity Capture-MS Homo sapiens
138 TIMP3 7078
Affinity Capture-MS Homo sapiens
139 HIST1H2AE 3012
Affinity Capture-MS Homo sapiens
140 C17orf59 54785
Affinity Capture-MS Homo sapiens
141 HSPA2 3306
Affinity Capture-MS Homo sapiens
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