Gene description for S1PR1
Gene name sphingosine-1-phosphate receptor 1
Gene symbol S1PR1
Other names/aliases CD363
CHEDG1
D1S3362
ECGF1
EDG-1
EDG1
S1P1
Species Homo sapiens
 Database cross references - S1PR1
ExoCarta ExoCarta_1901
Vesiclepedia VP_1901
Entrez Gene 1901
HGNC 3165
MIM 601974
UniProt P21453  
 S1PR1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for S1PR1
Molecular Function
    sphingolipid binding GO:0046625 IEA
    G-protein coupled receptor binding GO:0001664 IPI
    G-protein coupled receptor activity GO:0004930 TAS
    sphingosine-1-phosphate receptor activity GO:0038036 IDA
Biological Process
    transmission of nerve impulse GO:0019226 IEA
    positive regulation of cell migration GO:0030335 IEA
    sphingosine-1-phosphate signaling pathway GO:0003376 IDA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IEA
    lamellipodium assembly GO:0030032 ISS
    cardiac muscle tissue growth involved in heart morphogenesis GO:0003245 ISS
    negative regulation of stress fiber assembly GO:0051497 IEA
    positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway GO:0051482 IEA
    cell migration GO:0016477 ISS
    regulation of bone resorption GO:0045124 ISS
    angiogenesis GO:0001525 IEA
    blood vessel maturation GO:0001955 ISS
    positive regulation of positive chemotaxis GO:0050927 IEA
    G-protein coupled receptor signaling pathway GO:0007186 TAS
    brain development GO:0007420 IEA
    actin cytoskeleton reorganization GO:0031532 ISS
    neuron differentiation GO:0030182 IEA
    positive regulation of smooth muscle cell proliferation GO:0048661 IEA
    cell adhesion GO:0007155 TAS
    T cell migration GO:0072678 ISS
    regulation of bone mineralization GO:0030500 ISS
    heart trabecula morphogenesis GO:0061384 ISS
    chemotaxis GO:0006935 ISS
    endothelial cell differentiation GO:0045446 IEA
    adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway GO:0007193 IEA
    regulation of cell adhesion GO:0030155 IEA
Subcellular Localization
    plasma membrane GO:0005886 TAS
    intrinsic component of plasma membrane GO:0031226 IDA
    cytosol GO:0005829 IEA
    membrane raft GO:0045121 IEA
    endosome GO:0005768 IEA
    external side of plasma membrane GO:0009897 IEA
    integral component of membrane GO:0016021 IEA
 Experiment description of studies that identified S1PR1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for S1PR1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CAV1 857
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
2 HTR1D  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
3 PDGFRB 5159
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
4 GNAI1 2770
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
5 GNAI3 2773
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
6 HTR1A  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which S1PR1 is involved
PathwayEvidenceSource
G alpha (i) signalling events TAS Reactome
Lysosphingolipid and LPA receptors TAS Reactome





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