Gene description for PKD2
Gene name polycystic kidney disease 2 (autosomal dominant)
Gene symbol PKD2
Other names/aliases APKD2
PC2
PKD4
Pc-2
TRPP2
Species Homo sapiens
 Database cross references - PKD2
ExoCarta ExoCarta_5311
Vesiclepedia VP_5311
Entrez Gene 5311
HGNC 9009
MIM 173910
UniProt Q13563  
 PKD2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Urine 19056867    
 Gene ontology annotations for PKD2
Molecular Function
    receptor binding GO:0005102 IPI
    phosphoprotein binding GO:0051219 IPI
    ion channel binding GO:0044325 IPI
    cytoskeletal protein binding GO:0008092 IDA
    protein binding GO:0005515 IPI
    protein homodimerization activity GO:0042803 IDA
    muscle alpha-actinin binding GO:0051371 IEA
    calcium ion binding GO:0005509 ISS
    voltage-gated sodium channel activity GO:0005248 IDA
    voltage-gated calcium channel activity GO:0005245 IDA
    potassium channel activity GO:0005267 ISS
    actinin binding GO:0042805 IDA
    HLH domain binding GO:0043398 IPI
    voltage-gated ion channel activity GO:0005244 IDA
    ATPase binding GO:0051117 ISS
    identical protein binding GO:0042802 ISS
    voltage-gated cation channel activity GO:0022843 IDA
    calcium-induced calcium release activity GO:0048763 IDA
Biological Process
    metanephric part of ureteric bud development GO:0035502 IEP
    calcium ion transport GO:0006816 ISS
    regulation of calcium ion import GO:0090279 IDA
    heart development GO:0007507 IEP
    metanephric distal tubule development GO:0072235 IEP
    renal tubule morphogenesis GO:0061333 ISS
    metanephric smooth muscle tissue development GO:0072208 IEP
    cellular response to osmotic stress GO:0071470 IDA
    positive regulation of nitric oxide biosynthetic process GO:0045429 IMP
    liver development GO:0001889 IEP
    regulation of cAMP metabolic process GO:0030814 ISS
    cytoplasmic sequestering of transcription factor GO:0042994 IMP
    calcium ion transmembrane transport GO:0070588 IDA
    centrosome duplication GO:0051298 NAS
    metanephric mesenchyme development GO:0072075 IEP
    cell cycle arrest GO:0007050 ISS
    determination of left/right symmetry GO:0007368 ISS
    mesonephric duct development GO:0072177 IEP
    cellular response to reactive oxygen species GO:0034614 NAS
    cellular response to hydrostatic pressure GO:0071464 IDA
    neural tube development GO:0021915 IEP
    detection of mechanical stimulus GO:0050982 ISS
    regulation of cell proliferation GO:0042127 IMP
    JAK-STAT cascade GO:0007259 ISS
    potassium ion transmembrane transport GO:0071805 IEA
    aorta development GO:0035904 IEP
    embryonic placenta development GO:0001892 ISS
    cellular response to fluid shear stress GO:0071498 IMP
    metanephric cortical collecting duct development GO:0072219 IEP
    release of sequestered calcium ion into cytosol GO:0051209 IMP
    negative regulation of G1/S transition of mitotic cell cycle GO:2000134 IMP
    positive regulation of cell cycle arrest GO:0071158 IMP
    mesonephric tubule development GO:0072164 IEP
    detection of nodal flow GO:0003127 ISS
    heart looping GO:0001947 IMP
    metanephric cortex development GO:0072214 IEP
    metanephric S-shaped body morphogenesis GO:0072284 IEP
    positive regulation of inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity GO:0031587 IMP
    determination of liver left/right asymmetry GO:0071910 IMP
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IMP
    spinal cord development GO:0021510 IEP
    negative regulation of cell proliferation GO:0008285 NAS
    placenta blood vessel development GO:0060674 ISS
    negative regulation of ryanodine-sensitive calcium-release channel activity GO:0060315 ISS
    sodium ion transmembrane transport GO:0035725 IDA
    renal artery morphogenesis GO:0061441 IEP
    branching involved in ureteric bud morphogenesis GO:0001658 IEP
    metanephric ascending thin limb development GO:0072218 IEP
    positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle GO:0031659 IDA
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    ciliary membrane GO:0060170 IEA
    nonmotile primary cilium GO:0031513 ISS
    basal cortex GO:0045180 IDA
    filamentous actin GO:0031941 IDA
    mitotic spindle GO:0072686 IDA
    lamellipodium GO:0030027 IDA
    endoplasmic reticulum membrane GO:0005789 IDA
    integral component of plasma membrane GO:0005887 IDA
    cytosol GO:0005829 IEA
    motile primary cilium GO:0031512 ISS
    polycystin complex GO:0002133 ISS
    ciliary basal body GO:0036064 IDA
    cilium GO:0005929 ISS
    plasma membrane GO:0005886 IDA
    cytoplasm GO:0005737 IMP
    cell-cell junction GO:0005911 ISS
    basal plasma membrane GO:0009925 IDA
    integral component of lumenal side of endoplasmic reticulum membrane GO:0071556 IDA
    endoplasmic reticulum GO:0005783 IMP
    integral component of cytoplasmic side of endoplasmic reticulum membrane GO:0071458 IDA
 Experiment description of studies that identified PKD2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 234
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 235
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
7
Experiment ID 834
MISEV standards
✔
EM
EV Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 835
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 63
MISEV standards
✘ EV Biophysical techniques
✔
AQP2
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for PKD2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PKD1 5310
Invivo Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
2 TNNI3  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
3 PKD2 5311
Invivo Homo sapiens
Two-hybrid Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
4 TRPC1  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which PKD2 is involved
PathwayEvidenceSource
VxPx cargo-targeting to cilium TAS Reactome





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