Gene description for Ppp2r1a
Gene name protein phosphatase 2, regulatory subunit A, alpha
Gene symbol Ppp2r1a
Other names/aliases 6330556D22Rik
PP2A
PR65
Species Mus musculus
 Database cross references - Ppp2r1a
ExoCarta ExoCarta_51792
Entrez Gene 51792
UniProt Q76MZ3  
 Ppp2r1a identified in exosomes derived from the following tissue/cell type
Fibroblasts 23260141    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 17486113    
Pancreatic cells 19351151    
 Gene ontology annotations for Ppp2r1a
Molecular Function
    protein binding GO:0005515 IPI
    protein heterodimerization activity GO:0046982 ISO
    protein serine/threonine phosphatase activity GO:0004722 IDA
    antigen binding GO:0003823 ISO
Biological Process
    chromosome segregation GO:0007059 ISO
    positive regulation of extrinsic apoptotic signaling pathway in absence of ligand GO:2001241 IMP
    meiotic spindle elongation GO:0051232 IMP
    meiotic sister chromatid cohesion, centromeric GO:0051754 IMP
    mitotic sister chromatid separation GO:0051306 IMP
    regulation of meiotic cell cycle process involved in oocyte maturation GO:1903538 IMP
    female meiotic division GO:0007143 IMP
    peptidyl-serine dephosphorylation GO:0070262 IDA
Subcellular Localization
    protein phosphatase type 2A complex GO:0000159 ISO
    chromosome GO:0005694 IEA
    cytoplasm GO:0005737 IEA
    chromosome, centromeric region GO:0000775 ISO
    extracellular exosome GO:0070062 ISO
    cytosol GO:0005829 IDA
 Experiment description of studies that identified Ppp2r1a in exosomes
1
Experiment ID 210
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|FLOT1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 214
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 15
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
6
Experiment ID 188
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|UCHL1|HSP90
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Ppp2r1a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ptpra 19262
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
View the network image/svg+xml
 Pathways in which Ppp2r1a is involved
PathwayEvidenceSource
Anchoring of the basal body to the plasma membrane IEA Reactome
Beta-catenin phosphorylation cascade IEA Reactome
CTLA4 inhibitory signaling IEA Reactome
Cyclin A/B1 associated events during G2/M transition IEA Reactome
Cyclin D associated events in G1 IEA Reactome
DARPP-32 events IEA Reactome
Degradation of beta-catenin by the destruction complex IEA Reactome
disassembly of the destruction complex and recruitment of AXIN to the membrane IEA Reactome
ERK/MAPK targets IEA Reactome
ERK/MAPK targets IEA Reactome
ERKs are inactivated IEA Reactome
ERKs are inactivated IEA Reactome
Glycolysis IEA Reactome
Inhibition of replication initiation of damaged DNA by RB1/E2F1 IEA Reactome
Initiation of Nuclear Envelope Reformation IEA Reactome
Integration of energy metabolism IEA Reactome
Loss of Nlp from mitotic centrosomes IEA Reactome
Loss of proteins required for interphase microtubule organization from the centrosome IEA Reactome
MASTL Facilitates Mitotic Progression IEA Reactome
Mitotic Prometaphase IEA Reactome
Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) IEA Reactome
PP2A-mediated dephosphorylation of key metabolic factors IEA Reactome
Recruitment of mitotic centrosome proteins and complexes IEA Reactome
Regulation of PLK1 Activity at G2/M Transition IEA Reactome
Resolution of Sister Chromatid Cohesion IEA Reactome
RHO GTPases Activate Formins IEA Reactome
Separation of Sister Chromatids IEA Reactome
Spry regulation of FGF signaling IEA Reactome
Spry regulation of FGF signaling IEA Reactome
Spry regulation of FGF signaling IEA Reactome
Spry regulation of FGF signaling IEA Reactome





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