Gene description for Ppp2cb
Gene name protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform
Gene symbol Ppp2cb
Other names/aliases AI115466
D8Ertd766e
PP2Ac
Species Mus musculus
 Database cross references - Ppp2cb
ExoCarta ExoCarta_19053
Vesiclepedia VP_19053
Entrez Gene 19053
UniProt P62715  
 Ppp2cb identified in exosomes derived from the following tissue/cell type
Pancreatic cells 19351151    
 Gene ontology annotations for Ppp2cb
Molecular Function
    phosphoprotein phosphatase activity GO:0004721 ISO
    protein serine/threonine phosphatase activity GO:0004722 IBA
    protein serine/threonine phosphatase activity GO:0004722 ISO
    protein binding GO:0005515 IPI
    myosin phosphatase activity GO:0017018 IEA
    calmodulin-dependent protein phosphatase activity GO:0033192 IEA
    transmembrane transporter binding GO:0044325 IEA
    transmembrane transporter binding GO:0044325 ISO
    metal ion binding GO:0046872 IEA
    histone H2AXS140 phosphatase activity GO:0140791 IEA
    RNA polymerase II CTD heptapeptide repeat Y1 phosphatase activity GO:0180004 IEA
    RNA polymerase II CTD heptapeptide repeat T4 phosphatase activity GO:0180005 IEA
    RNA polymerase II CTD heptapeptide repeat S2 phosphatase activity GO:0180006 IEA
    RNA polymerase II CTD heptapeptide repeat S5 phosphatase activity GO:0180007 IEA
    RNA polymerase II CTD heptapeptide repeat S7 phosphatase activity GO:0180008 IEA
    MAP kinase serine/threonine phosphatase activity GO:1990439 IEA
Biological Process
    mitotic cell cycle GO:0000278 IBA
    chromatin remodeling GO:0006338 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IEA
    apoptotic mitochondrial changes GO:0008637 IMP
    response to lead ion GO:0010288 IEA
    response to lead ion GO:0010288 ISO
    regulation of gene expression GO:0010468 IMP
    negative regulation of gene expression GO:0010629 IEA
    negative regulation of gene expression GO:0010629 ISO
    negative regulation of gene expression GO:0010629 ISO
    negative regulation of tumor necrosis factor-mediated signaling pathway GO:0010804 IEA
    negative regulation of tumor necrosis factor-mediated signaling pathway GO:0010804 ISO
    negative regulation of NF-kappaB transcription factor activity GO:0032088 ISO
    response to endoplasmic reticulum stress GO:0034976 IMP
    response to hydrogen peroxide GO:0042542 IMP
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IMP
    negative regulation of Ras protein signal transduction GO:0046580 IEA
    negative regulation of Ras protein signal transduction GO:0046580 ISO
    response to antibiotic GO:0046677 IMP
Subcellular Localization
    protein phosphatase type 2A complex GO:0000159 IDA
    chromosome, centromeric region GO:0000775 IEA
    spindle pole GO:0000922 IEA
    nucleus GO:0005634 TAS
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
    FAR/SIN/STRIPAK complex GO:0090443 ISO
    FAR/SIN/STRIPAK complex GO:0090443 ISS
 Experiment description of studies that identified Ppp2cb in exosomes
1
Experiment ID 188
MISEV standards
EM
Biophysical techniques
GAPDH|UCHL1|HSP90
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Ppp2cb
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ppp2r1a 51792
Co-fractionation Mus musculus
Affinity Capture-Western Mus musculus
2 Kctd13  
Affinity Capture-MS Mus musculus
3 Mettl21e  
Affinity Capture-MS Mus musculus
4 Tnik  
Affinity Capture-MS Mus musculus
5 Eed  
Affinity Capture-MS Mus musculus
6 Ppp2r2a 71978
Affinity Capture-Western Mus musculus
7 Ttc1  
Co-fractionation Mus musculus
8 Dlg4  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
9 Fbxl16  
Affinity Capture-MS Mus musculus
10 MTNR1A  
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
11 Vcp 269523
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
12 Strn3  
Co-fractionation Mus musculus
13 Ppp2cb 19053
Co-fractionation Mus musculus
Co-fractionation Mus musculus
14 Tsc1  
Affinity Capture-MS Mus musculus
15 Cct5 12465
Co-fractionation Mus musculus
16 Igbp1  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
17 Tiprl  
Co-fractionation Mus musculus
18 Ywhaz 22631
Affinity Capture-MS Mus musculus
19 MTNR1B  
Reconstituted Complex Homo sapiens
20 Dusp1  
Affinity Capture-Western Mus musculus
View the network image/svg+xml
 Pathways in which Ppp2cb is involved
PathwayEvidenceSource
Adaptive Immune System IEA Reactome
Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal IEA Reactome
Amplification of signal from the kinetochores IEA Reactome
Antiviral mechanism by IFN-stimulated genes IEA Reactome
Beta-catenin phosphorylation cascade IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle Checkpoints IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Costimulation by the CD28 family IEA Reactome
CTLA4 inhibitory signaling IEA Reactome
Cyclin A/B1/B2 associated events during G2/M transition IEA Reactome
Cyclin D associated events in G1 IEA Reactome
Cytokine Signaling in Immune system IEA Reactome
DARPP-32 events IEA Reactome
Degradation of beta-catenin by the destruction complex IEA Reactome
Disassembly of the destruction complex and recruitment of AXIN to the membrane IEA Reactome
E2F mediated regulation of DNA replication IEA Reactome
EML4 and NUDC in mitotic spindle formation IEA Reactome
ERK/MAPK targets IEA Reactome
ERKs are inactivated IEA Reactome
G alpha (i) signalling events IEA Reactome
G1 Phase IEA Reactome
G1/S Transition IEA Reactome
G2/M Transition IEA Reactome
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway IEA Reactome
GPCR downstream signalling IEA Reactome
Hemostasis IEA Reactome
Immune System IEA Reactome
Inhibition of replication initiation of damaged DNA by RB1/E2F1 IEA Reactome
Innate Immune System IEA Reactome
Interferon Signaling IEA Reactome
Interleukin-17 signaling IEA Reactome
Intracellular signaling by second messengers IEA Reactome
M Phase IEA Reactome
MAP kinase activation IEA Reactome
MAPK family signaling cascades IEA Reactome
MAPK targets/ Nuclear events mediated by MAP kinases IEA Reactome
MAPK1/MAPK3 signaling IEA Reactome
Mitotic Anaphase IEA Reactome
Mitotic G1 phase and G1/S transition IEA Reactome
Mitotic G2-G2/M phases IEA Reactome
Mitotic Metaphase and Anaphase IEA Reactome
Mitotic Prometaphase IEA Reactome
Mitotic Spindle Checkpoint IEA Reactome
MyD88 cascade initiated on plasma membrane IEA Reactome
MyD88 dependent cascade initiated on endosome IEA Reactome
MyD88-independent TLR4 cascade IEA Reactome
MyD88:MAL(TIRAP) cascade initiated on plasma membrane IEA Reactome
Negative regulation of FGFR1 signaling IEA Reactome
Negative regulation of FGFR2 signaling IEA Reactome
Negative regulation of FGFR3 signaling IEA Reactome
Negative regulation of FGFR4 signaling IEA Reactome
Negative regulation of MAPK pathway IEA Reactome
Negative regulation of the PI3K/AKT network IEA Reactome
Nuclear Events (kinase and transcription factor activation) IEA Reactome
Opioid Signalling IEA Reactome
PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling IEA Reactome
PIP3 activates AKT signaling IEA Reactome
PKR-mediated signaling IEA Reactome
Platelet homeostasis IEA Reactome
Platelet sensitization by LDL IEA Reactome
RAF activation IEA Reactome
RAF/MAP kinase cascade IEA Reactome
Regulation of TP53 Activity IEA Reactome
Regulation of TP53 Degradation IEA Reactome
Regulation of TP53 Expression and Degradation IEA Reactome
Resolution of Sister Chromatid Cohesion IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate Formins IEA Reactome
RNA Polymerase II Transcription IEA Reactome
Separation of Sister Chromatids IEA Reactome
Signal Transduction IEA Reactome
Signaling by FGFR IEA Reactome
Signaling by FGFR1 IEA Reactome
Signaling by FGFR2 IEA Reactome
Signaling by FGFR3 IEA Reactome
Signaling by FGFR4 IEA Reactome
Signaling by GPCR IEA Reactome
Signaling by Interleukins IEA Reactome
Signaling by NTRK1 (TRKA) IEA Reactome
Signaling by NTRKs IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
Signaling by WNT IEA Reactome
Spry regulation of FGF signaling IEA Reactome
TCF dependent signaling in response to WNT IEA Reactome
Toll Like Receptor 10 (TLR10) Cascade IEA Reactome
Toll Like Receptor 2 (TLR2) Cascade IEA Reactome
Toll Like Receptor 3 (TLR3) Cascade IEA Reactome
Toll Like Receptor 4 (TLR4) Cascade IEA Reactome
Toll Like Receptor 5 (TLR5) Cascade IEA Reactome
Toll Like Receptor 7/8 (TLR7/8) Cascade IEA Reactome
Toll Like Receptor 9 (TLR9) Cascade IEA Reactome
Toll Like Receptor TLR1:TLR2 Cascade IEA Reactome
Toll Like Receptor TLR6:TLR2 Cascade IEA Reactome
Toll-like Receptor Cascades IEA Reactome
TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation IEA Reactome
Transcriptional Regulation by TP53 IEA Reactome
TRIF (TICAM1)-mediated TLR4 signaling IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here