Gene ontology annotations for Ywhaz
Experiment description of studies that identified Ywhaz in exosomes
1
Experiment ID
10
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD9|MHCII|MHCI|CD86|LAMP2
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry [MALDI TOF] Mass spectrometry [QTOF]
PubMed ID
11390481
Organism
Mus musculus
Experiment description
Proteomic analysis of dendritic cell-derived exosomes: a secreted subcellular compartment distinct from apoptotic vesicles.
Authors
Thery C, Boussac M, Veron P, Ricciardi-Castagnoli P, Raposo G, Garin J, Amigorena S.
Journal name
JIMMU
Publication year
2001
Sample
Dendritic cells
Sample name
D1
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.15-1.18 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [MALDI TOF] Mass spectrometry [QTOF] Western blotting
2
Experiment ID
73
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|HSP90
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
18494037
Organism
Mus musculus
Experiment description
Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors
Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ
Journal name
ELEC
Publication year
2008
Sample
Embryonic fibroblasts
Sample name
NIH3T3
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LCQ DECA] Western blotting
3
Experiment ID
210
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD81|FLOT1
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23260141
Organism
Mus musculus
Experiment description
Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors
Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name
Cell
Publication year
2012
Sample
Fibroblasts
Sample name
Normal-Fibroblasts (L cells)
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
4
Experiment ID
15
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
17486113
Organism
Mus musculus Homo sapiens
Experiment description
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors
Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name
NCB
Publication year
2007
Sample
Mast cells
Sample name
MC9 Bone marrow-derived mast cells HMC-1
Isolation/purification methods
Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.11-1.21 g/mL
Molecules identified in the study
Protein mRNA miRNA
Methods used in the study
Mass spectrometry [MALDI TOF] Western blotting Microarray miRCURY LNA Array
5
Experiment ID
15
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
mrna
Identification method
Microarray
PubMed ID
17486113
Organism
Mus musculus Homo sapiens
Experiment description
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors
Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name
NCB
Publication year
2007
Sample
Mast cells
Sample name
MC9 Bone marrow-derived mast cells HMC-1
Isolation/purification methods
Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.11-1.21 g/mL
Molecules identified in the study
Protein mRNA miRNA
Methods used in the study
Mass spectrometry [MALDI TOF] Western blotting Microarray miRCURY LNA Array
6
Experiment ID
14
ISEV standards
✔
EM
EV Biophysical techniques
✔
RAB7|RAB11
EV Cytosolic markers
✔
CD9|CD63|LAMP1|LAMP2
EV Membrane markers
✔
DNM
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
16081791
Organism
Mus musculus
Experiment description
Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors
Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L
Journal name
JIMMU
Publication year
2005
Sample
Microglia
Sample name
N9
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LCQ DECA XP] Western blotting
7
Experiment ID
39
ISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|HSC70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
15210972
Organism
Mus musculus
Experiment description
Cells release prions in association with exosomes.
Authors
Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G
Journal name
PNAS
Publication year
2004
Sample
Mov neuroglial cells
Sample name
Mov neuroglial cell
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.14 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QSTAR] Western blotting Immunoelectron Microscopy
8
Experiment ID
33
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
21136642
Organism
Mus musculus
Experiment description
Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors
Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J
Journal name
PROTEOMICS_CL
Publication year
2007
Sample
Oligodendrocytes
Sample name
Oligodendrocytes Oli-neu
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.10-1.14 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QTOF] Western blotting Immunoelectron Microscopy
9
Experiment ID
188
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|UCHL1|HSP90
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
19351151
Organism
Mus musculus
Experiment description
Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors
Lee HS, Jeong J, Lee KJ.
Journal name
J Proteome Res
Publication year
2009
Sample
Pancreatic cells
Sample name
Pancreatic beta cell (NIT-1)
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
Protein-protein interactions for Ywhaz
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
Mlf1
Affinity Capture-Western
Mus musculus
Two-hybrid
Mus musculus
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Pathways in which Ywhaz is involved