Gene description for Hspa9
Gene name heat shock protein 9
Gene symbol Hspa9
Other names/aliases 74kDa
Csa
Grp75
Hsc74
Hsp74
Hsp74a
Hspa9a
Mortalin
Mot-2
Mot2
Mthsp70
Pbp74
Species Mus musculus
 Database cross references - Hspa9
ExoCarta ExoCarta_15526
Entrez Gene 15526
UniProt P38647  
 Hspa9 identified in exosomes derived from the following tissue/cell type
Neural stem cells 25242146    
Pancreatic cells 19351151    
 Gene ontology annotations for Hspa9
Molecular Function
    protein binding GO:0005515 IPI
    fibroblast growth factor binding GO:0017134 ISO
    heat shock protein binding GO:0031072 ISO
    enzyme binding GO:0019899 IPI
    ubiquitin protein ligase binding GO:0031625 ISO
    poly(A) RNA binding GO:0044822 ISO
    unfolded protein binding GO:0051082 IEA
    ATP binding GO:0005524 IEA
    nucleotide binding GO:0000166 IEA
Biological Process
    protein export from nucleus GO:0006611 IDA
    protein folding GO:0006457 IEA
Subcellular Localization
    cytoplasm GO:0005737 TAS
    extracellular exosome GO:0070062 ISO
    myelin sheath GO:0043209 IDA
    focal adhesion GO:0005925 ISO
    mitochondrion GO:0005739 ISO
    mitochondrial nucleoid GO:0042645 ISO
    nucleus GO:0005634 IEA
 Experiment description of studies that identified Hspa9 in exosomes
1
Experiment ID 263
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-γ via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th1 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|UCHL1|HSP90
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Hspa9
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Hspa9 is involved
PathwayEvidenceSource
Mitochondrial protein import IEA Reactome





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