Gene description for Mdh2
Gene name malate dehydrogenase 2, NAD (mitochondrial)
Gene symbol Mdh2
Other names/aliases MDH
Mdh-2
Mor-1
Mor1
Species Mus musculus
 Database cross references - Mdh2
ExoCarta ExoCarta_17448
Vesiclepedia VP_17448
Entrez Gene 17448
UniProt P08249  
 Mdh2 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Mdh2
Molecular Function
    malate dehydrogenase activity GO:0016615 ISO
    oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor GO:0016616 IEA
    catalytic activity GO:0003824 IEA
    protein self-association GO:0043621 ISO
    malate dehydrogenase (NADP+) activity GO:0046554 ISO
    oxidoreductase activity GO:0016491 IEA
    poly(A) RNA binding GO:0044822 ISO
    L-malate dehydrogenase activity GO:0030060 ISO
Biological Process
    carboxylic acid metabolic process GO:0019752 IEA
    tricarboxylic acid cycle GO:0006099 ISO
    carbohydrate metabolic process GO:0005975 IEA
    malate metabolic process GO:0006108 ISO
    oxidation-reduction process GO:0055114 ISA
    oxaloacetate metabolic process GO:0006107 ISO
    NADH metabolic process GO:0006734 ISO
    internal protein amino acid acetylation GO:0006475 ISO
Subcellular Localization
    mitochondrial inner membrane GO:0005743 IDA
    myelin sheath GO:0043209 IDA
    nucleoplasm GO:0005654 ISO
    plasma membrane GO:0005886 ISO
    nucleus GO:0005634 ISO
    extracellular exosome GO:0070062 ISO
    mitochondrial matrix GO:0005759 ISO
    membrane GO:0016020 IMP
    mitochondrion GO:0005739 ISO
 Experiment description of studies that identified Mdh2 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD81|FLOT1
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 188
MISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|UCHL1|HSP90
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Mdh2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Mdh2 is involved
PathwayEvidenceSource
Citric acid cycle (TCA cycle) IEA Reactome
Gluconeogenesis IEA Reactome





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