Gene description for Nnt
Gene name nicotinamide nucleotide transhydrogenase
Gene symbol Nnt
Other names/aliases 4930423F13Rik
AI323702
BB168308
Species Mus musculus
 Database cross references - Nnt
ExoCarta ExoCarta_18115
Vesiclepedia VP_18115
Entrez Gene 18115
 Nnt identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Nnt
Molecular Function
    proton-translocating NAD(P)+ transhydrogenase activity GO:0008750 IEA
    oxidoreductase activity GO:0016491 IEA
    NADP binding GO:0050661 IBA
    NADP binding GO:0050661 ISO
Biological Process
    negative regulation of protein phosphorylation GO:0001933 ISO
    NADPH regeneration GO:0006740 IBA
    NADPH regeneration GO:0006740 ISO
    positive regulation of mitochondrial membrane potential GO:0010918 ISO
    intracellular oxygen homeostasis GO:0032364 ISO
    negative regulation of apoptotic process GO:0043066 ISO
    cell redox homeostasis GO:0045454 ISO
    reactive oxygen species metabolic process GO:0072593 ISO
    reactive oxygen species metabolic process GO:0072593 ISS
    cellular oxidant detoxification GO:0098869 ISO
    proton transmembrane transport GO:1902600 IEA
    positive regulation of hydrogen peroxide catabolic process GO:1903285 ISO
Subcellular Localization
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 ISO
    mitochondrial inner membrane GO:0005743 HDA
 Experiment description of studies that identified Nnt in exosomes
1
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Nnt
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Etfdh  
Cross-Linking-MS (XL-MS) Mus musculus
2 Atp5j  
Cross-Linking-MS (XL-MS) Mus musculus
3 Atp5a1 11946
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
4 Nnt 18115
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
5 Usp7  
Affinity Capture-MS Mus musculus
6 Sdhc  
Cross-Linking-MS (XL-MS) Mus musculus
7 Sdha 66945
Cross-Linking-MS (XL-MS) Mus musculus
8 Slc25a4 11739
Cross-Linking-MS (XL-MS) Mus musculus
9 Cs 12974
Cross-Linking-MS (XL-MS) Mus musculus
10 Hspb2 69253
Affinity Capture-MS Mus musculus
11 Mdh2 17448
Cross-Linking-MS (XL-MS) Mus musculus
12 Atp5b 11947
Cross-Linking-MS (XL-MS) Mus musculus
13 Aco2 11429
Cross-Linking-MS (XL-MS) Mus musculus
View the network image/svg+xml
 Pathways in which Nnt is involved
No pathways found





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