Gene description for Slc25a4
Gene name solute carrier family 25 (mitochondrial carrier, adenine nucleotide translocator), member 4
Gene symbol Slc25a4
Other names/aliases AU019225
Ant1
Species Mus musculus
 Database cross references - Slc25a4
ExoCarta ExoCarta_11739
Vesiclepedia VP_11739
Entrez Gene 11739
UniProt P48962  
 Slc25a4 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Slc25a4
Molecular Function
    ATP:ADP antiporter activity GO:0005471 IDA
    ATP:ADP antiporter activity GO:0005471 IMP
    ATP:ADP antiporter activity GO:0005471 ISO
    protein binding GO:0005515 IPI
    proton transmembrane transporter activity GO:0015078 IDA
    oxidative phosphorylation uncoupler activity GO:0017077 IDA
    enzyme binding GO:0019899 ISO
Biological Process
    apoptotic mitochondrial changes GO:0008637 IGI
    negative regulation of cardiac muscle cell apoptotic process GO:0010667 ISO
    ADP transport GO:0015866 ISO
    ADP transport GO:0015866 ISS
    regulation of mitochondrial membrane permeability GO:0046902 IMP
    negative regulation of necroptotic process GO:0060546 IEA
    negative regulation of necroptotic process GO:0060546 ISO
    positive regulation of cell growth involved in cardiac muscle cell development GO:0061051 ISO
    mitochondrial ADP transmembrane transport GO:0140021 IDA
    mitochondrial ADP transmembrane transport GO:0140021 IMP
    mitochondrial ADP transmembrane transport GO:0140021 ISO
    negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway GO:1901029 IBA
    positive regulation of mitophagy GO:1901526 IDA
    negative regulation of mitochondrial membrane permeability involved in apoptotic process GO:1902109 ISO
    proton transmembrane transport GO:1902600 IEA
    mitochondrial ATP transmembrane transport GO:1990544 IDA
    mitochondrial ATP transmembrane transport GO:1990544 IMP
    mitochondrial ATP transmembrane transport GO:1990544 ISO
    adaptive thermogenesis GO:1990845 IMP
Subcellular Localization
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 ISO
    mitochondrial outer membrane GO:0005741 ISS
    mitochondrial inner membrane GO:0005743 HDA
    mitochondrial inner membrane GO:0005743 ISO
    mitochondrial inner membrane GO:0005743 ISS
    mitochondrial permeability transition pore complex GO:0005757 IMP
    membrane GO:0016020 ISO
    mitochondrial membrane GO:0031966 ISS
    myelin sheath GO:0043209 HDA
    membrane raft GO:0045121 ISO
 Experiment description of studies that identified Slc25a4 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Slc25a4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Got2 14719
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
2 Nphs2  
Affinity Capture-MS Mus musculus
3 BCLAF1 9774
Affinity Capture-MS Homo sapiens
4 Ubc  
Reconstituted Complex Mus musculus
5 Slc25a3 18674
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
6 Atp5j  
Cross-Linking-MS (XL-MS) Mus musculus
7 Idh2 269951
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
8 Etfdh  
Cross-Linking-MS (XL-MS) Mus musculus
9 Etfb 110826
Cross-Linking-MS (XL-MS) Mus musculus
10 Slc25a13 50799
Cross-Linking-MS (XL-MS) Mus musculus
11 Hadha  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
12 Slc25a4 11739
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
13 Cox4i1 12857
Cross-Linking-MS (XL-MS) Mus musculus
14 Ckmt2  
Cross-Linking-MS (XL-MS) Mus musculus
15 Ndufa9 66108
Cross-Linking-MS (XL-MS) Mus musculus
16 Pls3 102866
Cross-Linking-MS (XL-MS) Mus musculus
17 Gbas  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
18 Cox7b  
Cross-Linking-MS (XL-MS) Mus musculus
19 Cycs 13063
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
20 Uqcrh  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
21 Ndufs7  
Cross-Linking-MS (XL-MS) Mus musculus
22 Mpc2  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
23 D10Jhu81e  
Cross-Linking-MS (XL-MS) Mus musculus
24 Atxn1  
Proximity Label-MS Mus musculus
25 Sdhc  
Cross-Linking-MS (XL-MS) Mus musculus
26 Acadvl 11370
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
27 Kctd13  
Affinity Capture-MS Mus musculus
28 Thrb  
Affinity Capture-MS Mus musculus
29 Cox6c  
Cross-Linking-MS (XL-MS) Mus musculus
Co-fractionation Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
30 Etfa 110842
Cross-Linking-MS (XL-MS) Mus musculus
31 Fam162a  
Cross-Linking-MS (XL-MS) Mus musculus
32 Tfe3  
Affinity Capture-MS Mus musculus
33 Cox6b1  
Co-fractionation Mus musculus
34 ARL2BP 23568
Reconstituted Complex Homo sapiens
35 Pdha1  
Co-fractionation Mus musculus
36 Decr1  
Cross-Linking-MS (XL-MS) Mus musculus
37 Atp5l  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
38 Nnt 18115
Cross-Linking-MS (XL-MS) Mus musculus
39 Ncoa4  
Cross-Linking-MS (XL-MS) Mus musculus
40 Atp5a1 11946
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
41 Uqcrb  
Cross-Linking-MS (XL-MS) Mus musculus
42 Ndufa7  
Cross-Linking-MS (XL-MS) Mus musculus
43 Ndufa8 68375
Cross-Linking-MS (XL-MS) Mus musculus
44 Tmem173  
Proximity Label-MS Mus musculus
45 Aldh4a1  
Cross-Linking-MS (XL-MS) Mus musculus
46 Cox5a  
Cross-Linking-MS (XL-MS) Mus musculus
47 Pfkm 18642
Co-fractionation Mus musculus
48 Slc25a5 11740
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
49 Idh3a 67834
Co-fractionation Mus musculus
50 Mrpl24  
Cross-Linking-MS (XL-MS) Mus musculus
51 Immt  
Cross-Linking-MS (XL-MS) Mus musculus
52 Cyct  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
53 Fh1 14194
Cross-Linking-MS (XL-MS) Mus musculus
54 Cs 12974
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
55 Hadhb  
Cross-Linking-MS (XL-MS) Mus musculus
56 Cpt2  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
57 Atp5b 11947
Cross-Linking-MS (XL-MS) Mus musculus
58 Prkce  
Co-fractionation Mus musculus
59 Apoo  
Cross-Linking-MS (XL-MS) Mus musculus
60 Atp5f1  
Cross-Linking-MS (XL-MS) Mus musculus
61 Sdhb 67680
Cross-Linking-MS (XL-MS) Mus musculus
Co-fractionation Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
62 Uqcrq  
Cross-Linking-MS (XL-MS) Mus musculus
63 Acaa2  
Cross-Linking-MS (XL-MS) Mus musculus
64 Ndufa4  
Cross-Linking-MS (XL-MS) Mus musculus
Co-fractionation Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
65 Eed  
Affinity Capture-MS Mus musculus
66 Tecr  
Cross-Linking-MS (XL-MS) Mus musculus
67 Atp5o 28080
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
68 Mapt  
Affinity Capture-MS Mus musculus
69 Aco2 11429
Cross-Linking-MS (XL-MS) Mus musculus
Co-fractionation Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
70 Uqcrc2 67003
Cross-Linking-MS (XL-MS) Mus musculus
Co-fractionation Mus musculus
71 Ndufv1  
Cross-Linking-MS (XL-MS) Mus musculus
72 Fancd2  
Affinity Capture-MS Mus musculus
73 Mdh2 17448
Co-fractionation Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
74 Vdac3 22335
Co-fractionation Mus musculus
75 Uqcrfs1 66694
Co-fractionation Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
76 Lipc  
Affinity Capture-MS Mus musculus
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