Gene description for Slc25a5
Gene name solute carrier family 25 (mitochondrial carrier, adenine nucleotide translocator), member 5
Gene symbol Slc25a5
Other names/aliases Ant2
Species Mus musculus
 Database cross references - Slc25a5
ExoCarta ExoCarta_11740
Entrez Gene 11740
UniProt P51881  
 Slc25a5 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
Mov neuroglial cells 15210972    
Pancreatic cells 19351151    
 Gene ontology annotations for Slc25a5
Molecular Function
    ubiquitin protein ligase binding GO:0031625 ISO
    transporter activity GO:0005215 IEA
    poly(A) RNA binding GO:0044822 ISO
Biological Process
    transmembrane transport GO:0055085 IEA
    transport GO:0006810 IEA
    chromosome segregation GO:0007059 IEA
    negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway GO:1901029 ISO
    positive regulation of cell proliferation GO:0008284 ISO
Subcellular Localization
    membrane GO:0016020 ISO
    integral component of membrane GO:0016021 IEA
    mitochondrial inner membrane GO:0005743 ISO
    myelin sheath GO:0043209 IDA
    nucleus GO:0005634 ISO
    mitochondrial nucleoid GO:0042645 ISO
    extracellular exosome GO:0070062 ISO
    MMXD complex GO:0071817 ISO
    mitochondrion GO:0005739 ISO
 Experiment description of studies that identified Slc25a5 in exosomes
1
Experiment ID 15
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
2
Experiment ID 39
ISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|HSC70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
3
Experiment ID 188
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|UCHL1|HSP90
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Slc25a5
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Slc25a5 is involved
PathwayEvidenceSource
Regulation of insulin secretion IEA Reactome





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