Gene description for Idh2
Gene name isocitrate dehydrogenase 2 (NADP+), mitochondrial
Gene symbol Idh2
Other names/aliases E430004F23
IDPm
Idh-2
Species Mus musculus
 Database cross references - Idh2
ExoCarta ExoCarta_269951
Entrez Gene 269951
UniProt P54071  
 Idh2 identified in exosomes derived from the following tissue/cell type
Neural stem cells 25242146    
Pancreatic cells 19351151    
 Gene ontology annotations for Idh2
Molecular Function
    NAD binding GO:0051287 IEA
    oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor GO:0016616 IEA
    oxidoreductase activity GO:0016491 IEA
    metal ion binding GO:0046872 IEA
    magnesium ion binding GO:0000287 IEA
    isocitrate dehydrogenase (NADP+) activity GO:0004450 ISO
Biological Process
    tricarboxylic acid cycle GO:0006099 IEA
    glyoxylate cycle GO:0006097 IEA
    oxidation-reduction process GO:0055114 ISO
    isocitrate metabolic process GO:0006102 IEA
Subcellular Localization
    mitochondrion GO:0005739 ISO
    mitochondrial inner membrane GO:0005743 IDA
    extracellular exosome GO:0070062 ISO
 Experiment description of studies that identified Idh2 in exosomes
1
Experiment ID 263
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-γ via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th1 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|UCHL1|HSP90
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Idh2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Idh2 is involved
PathwayEvidenceSource
Citric acid cycle (TCA cycle) IEA Reactome





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