Gene description for Dld
Gene name dihydrolipoamide dehydrogenase
Gene symbol Dld
Other names/aliases -
Species Mus musculus
 Database cross references - Dld
ExoCarta ExoCarta_13382
Vesiclepedia VP_13382
Entrez Gene 13382
UniProt O08749  
 Dld identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Melanoma cells 34957415    
Melanoma cells 34957415    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Dld
Molecular Function
    dihydrolipoyl dehydrogenase activity GO:0004148 IBA
    dihydrolipoyl dehydrogenase activity GO:0004148 IMP
    dihydrolipoyl dehydrogenase activity GO:0004148 ISO
    dihydrolipoyl dehydrogenase activity GO:0004148 ISS
    protein binding GO:0005515 IPI
    pyruvate dehydrogenase (NAD+) activity GO:0034604 IC
    pyruvate dehydrogenase (NAD+) activity GO:0034604 ISO
    lipoamide binding GO:0043544 ISO
    branched-chain alpha-keto acid dehydrogenase activity GO:0047101 IEA
    branched-chain alpha-keto acid dehydrogenase activity GO:0047101 ISO
    flavin adenine dinucleotide binding GO:0050660 IBA
    flavin adenine dinucleotide binding GO:0050660 IEA
    flavin adenine dinucleotide binding GO:0050660 ISO
    NAD binding GO:0051287 ISO
Biological Process
    acetyl-CoA biosynthetic process from pyruvate GO:0006086 IC
    acetyl-CoA biosynthetic process from pyruvate GO:0006086 IMP
    acetyl-CoA biosynthetic process from pyruvate GO:0006086 ISO
    acetyl-CoA biosynthetic process from pyruvate GO:0006086 ISO
    pyruvate metabolic process GO:0006090 IBA
    2-oxoglutarate metabolic process GO:0006103 IBA
    2-oxoglutarate metabolic process GO:0006103 ISO
    mitochondrial electron transport, NADH to ubiquinone GO:0006120 IMP
    proteolysis GO:0006508 IDA
    gastrulation GO:0007369 IMP
    branched-chain amino acid catabolic process GO:0009083 IEA
    branched-chain amino acid catabolic process GO:0009083 ISO
    lipoate metabolic process GO:0009106 ISO
    regulation of membrane potential GO:0042391 IMP
    sperm capacitation GO:0048240 IDA
    dihydrolipoamide metabolic process GO:0051068 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 ISO
    mitochondrial matrix GO:0005759 IDA
    cilium GO:0005929 IDA
    motile cilium GO:0031514 IEA
    acrosomal matrix GO:0043159 IDA
    myelin sheath GO:0043209 HDA
    oxoglutarate dehydrogenase complex GO:0045252 IBA
    oxoglutarate dehydrogenase complex GO:0045252 IMP
    oxoglutarate dehydrogenase complex GO:0045252 ISO
    oxoglutarate dehydrogenase complex GO:0045252 ISS
    pyruvate dehydrogenase complex GO:0045254 IMP
    pyruvate dehydrogenase complex GO:0045254 ISO
    branched-chain alpha-ketoacid dehydrogenase complex GO:0160157 IEA
    oxoadipate dehydrogenase complex GO:0160167 IEA
    acetyltransferase complex GO:1902493 IMP
 Experiment description of studies that identified Dld in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 1117
MISEV standards
✘ Biophysical techniques
✔
Cd81|Icam1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 1118
MISEV standards
✘ Biophysical techniques
✔
Cd81|Icam1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Dld
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Nphs2  
Affinity Capture-MS Mus musculus
2 Atp5h 71679
Cross-Linking-MS (XL-MS) Mus musculus
3 Pdha2  
Cross-Linking-MS (XL-MS) Mus musculus
4 Atp5j  
Cross-Linking-MS (XL-MS) Mus musculus
5 Apoo  
Co-fractionation Mus musculus
6 Hspb2 69253
Affinity Capture-MS Mus musculus
7 Kcnma1  
Affinity Capture-MS Mus musculus
8 Atp5f1  
Cross-Linking-MS (XL-MS) Mus musculus
9 Atxn1  
Affinity Capture-MS Mus musculus
10 Kctd13  
Affinity Capture-MS Mus musculus
11 Ndufc2  
Co-fractionation Mus musculus
12 Ndufs6  
Co-fractionation Mus musculus
13 Htt  
Affinity Capture-MS Mus musculus
14 HSD17B10 3028
Affinity Capture-MS Homo sapiens
15 Dld 13382
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
16 Sucla2  
Co-fractionation Mus musculus
17 D10Jhu81e  
Cross-Linking-MS (XL-MS) Mus musculus
18 Ndufa7  
Co-fractionation Mus musculus
19 Ogdh 18293
Cross-Linking-MS (XL-MS) Mus musculus
20 Sdha 66945
Co-fractionation Mus musculus
21 Cs 12974
Co-fractionation Mus musculus
22 Tnfaip8  
Co-fractionation Mus musculus
23 Suclg1  
Co-fractionation Mus musculus
24 Slc25a11  
Co-fractionation Mus musculus
25 Idh2 269951
Cross-Linking-MS (XL-MS) Mus musculus
26 Pdhx 27402
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
27 Sstr3  
Proximity Label-MS Mus musculus
28 Sdhb 67680
Co-fractionation Mus musculus
29 Uqcrc2 67003
Co-fractionation Mus musculus
30 Tia1  
Affinity Capture-MS Mus musculus
31 Dlst  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
32 Fancd2  
Affinity Capture-MS Mus musculus
33 Mdh2 17448
Cross-Linking-MS (XL-MS) Mus musculus
34 Uqcrfs1 66694
Co-fractionation Mus musculus
35 Dlat  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
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