Gene description for Etfb
Gene name electron transferring flavoprotein, beta polypeptide
Gene symbol Etfb
Other names/aliases 0610009I16Rik
2810441H06Rik
Species Mus musculus
 Database cross references - Etfb
ExoCarta ExoCarta_110826
Vesiclepedia VP_110826
Entrez Gene 110826
UniProt Q9DCW4  
 Etfb identified in sEVs derived from the following tissue/cell type
Melanoma cells 34957415    
Melanoma cells 34957415    
Neural stem cells 25242146    
Pancreatic cells 19351151    
 Gene ontology annotations for Etfb
Molecular Function
    nucleotide binding GO:0000166 IEA
    electron transfer activity GO:0009055 IBA
    electron transfer activity GO:0009055 ISO
    electron transfer activity GO:0009055 ISS
    electron transfer activity GO:0009055 TAS
Biological Process
    amino acid catabolic process GO:0009063 ISO
    respiratory electron transport chain GO:0022904 ISO
    fatty acid beta-oxidation using acyl-CoA dehydrogenase GO:0033539 IBA
    fatty acid beta-oxidation using acyl-CoA dehydrogenase GO:0033539 IMP
    fatty acid beta-oxidation using acyl-CoA dehydrogenase GO:0033539 ISO
    fatty acid beta-oxidation using acyl-CoA dehydrogenase GO:0033539 ISS
Subcellular Localization
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 ISO
    mitochondrial matrix GO:0005759 ISO
    mitochondrial matrix GO:0005759 ISS
    mitochondrial matrix GO:0005759 NAS
    mitochondrial electron transfer flavoprotein complex GO:0017133 TAS
    electron transfer flavoprotein complex GO:0045251 ISO
 Experiment description of studies that identified Etfb in sEVs
1
Experiment ID 1117
MISEV standards
✘ Biophysical techniques
✔
Cd81|Icam1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 1118
MISEV standards
✘ Biophysical techniques
✔
Cd81|Icam1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 263
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th1 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Etfb
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Etfdh  
Cross-Linking-MS (XL-MS) Mus musculus
2 Kctd13  
Affinity Capture-MS Mus musculus
3 Hsdl2  
Co-fractionation Mus musculus
4 Etfb 110826
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
5 Slc25a4 11739
Cross-Linking-MS (XL-MS) Mus musculus
6 Kcnma1  
Affinity Capture-MS Mus musculus
7 Atp5f1  
Cross-Linking-MS (XL-MS) Mus musculus
8 Atxn1  
Affinity Capture-MS Mus musculus
9 Idh2 269951
Cross-Linking-MS (XL-MS) Mus musculus
10 Etfa 110842
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
11 Suclg2  
Co-fractionation Mus musculus
12 Htt  
Affinity Capture-MS Mus musculus
13 HSD17B10 3028
Affinity Capture-MS Homo sapiens
14 Atp5b 11947
Cross-Linking-MS (XL-MS) Mus musculus
15 Decr1  
Co-fractionation Mus musculus
16 Csl  
Cross-Linking-MS (XL-MS) Mus musculus
17 Lyrm5  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
18 Atp5a1 11946
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
19 Acadm 11364
Co-fractionation Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
20 Atp5h 71679
Cross-Linking-MS (XL-MS) Mus musculus
21 Abcd2  
Cross-Linking-MS (XL-MS) Mus musculus
22 Aco2 11429
Cross-Linking-MS (XL-MS) Mus musculus
23 Fancd2  
Affinity Capture-MS Mus musculus
24 Mdh2 17448
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
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