Gene description for Atp5a1
Gene name ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1
Gene symbol Atp5a1
Other names/aliases AI035633
AL022851
AL023067
Atpm
D18Ertd206e
Mom2
Species Mus musculus
 Database cross references - Atp5a1
ExoCarta ExoCarta_11946
Vesiclepedia VP_11946
Entrez Gene 11946
UniProt Q03265  
 Atp5a1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Atp5a1
Molecular Function
    nucleotide binding GO:0000166 IEA
    ATPase activity GO:0016887 ISO
    MHC class I protein binding GO:0042288 ISO
    hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances GO:0016820 IEA
    ATP binding GO:0005524 ISO
    poly(A) RNA binding GO:0044822 ISO
    proton-transporting ATPase activity, rotational mechanism GO:0046961 IEA
    ADP binding GO:0043531 ISO
    proton-transporting ATP synthase activity, rotational mechanism GO:0046933 IMP
Biological Process
    ATP biosynthetic process GO:0006754 IEA
    transport GO:0006810 IEA
    ATP synthesis coupled proton transport GO:0015986 IEA
    ion transport GO:0006811 IEA
    negative regulation of endothelial cell proliferation GO:0001937 ISO
    embryo development GO:0009790 IMP
    ATP hydrolysis coupled proton transport GO:0015991 IEA
    hydrogen ion transmembrane transport GO:1902600 IMP
    proton transport GO:0015992 IEA
    lipid metabolic process GO:0006629 IMP
    ATP metabolic process GO:0046034 ISO
Subcellular Localization
    mitochondrial inner membrane GO:0005743 ISO
    mitochondrial proton-transporting ATP synthase complex GO:0005753 ISO
    myelin sheath GO:0043209 IDA
    COP9 signalosome GO:0008180 ISO
    mitochondrion GO:0005739 ISO
    plasma membrane GO:0005886 ISO
    proton-transporting ATP synthase complex, catalytic core F(1) GO:0045261 ISO
    extracellular exosome GO:0070062 ISO
    proton-transporting two-sector ATPase complex, catalytic domain GO:0033178 IEA
    mitochondrial proton-transporting ATP synthase complex, catalytic core F(1) GO:0000275 ISO
    membrane GO:0016020 ISO
 Experiment description of studies that identified Atp5a1 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
MISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|UCHL1|HSP90
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Atp5a1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Atp5a1 is involved
PathwayEvidenceSource
Formation of ATP by chemiosmotic coupling IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here