Gene description for Fh1
Gene name fumarate hydratase 1
Gene symbol Fh1
Other names/aliases Fh
Fh-1
Species Mus musculus
 Database cross references - Fh1
ExoCarta ExoCarta_14194
Vesiclepedia VP_14194
Entrez Gene 14194
UniProt P97807  
 Fh1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Fh1
Molecular Function
    fumarate hydratase activity GO:0004333 ISO
    lyase activity GO:0016829 IEA
    catalytic activity GO:0003824 IEA
Biological Process
    malate metabolic process GO:0006108 ISO
    homeostasis of number of cells within a tissue GO:0048873 IMP
    fumarate metabolic process GO:0006106 ISO
    tricarboxylic acid cycle GO:0006099 ISO
Subcellular Localization
    tricarboxylic acid cycle enzyme complex GO:0045239 IEA
    cytoplasm GO:0005737 IEA
    mitochondrion GO:0005739 ISO
    extracellular exosome GO:0070062 ISO
 Experiment description of studies that identified Fh1 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Fh1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Fh1 is involved
PathwayEvidenceSource
Citric acid cycle (TCA cycle) IEA Reactome





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