Gene description for Xpo1
Gene name exportin 1, CRM1 homolog (yeast)
Gene symbol Xpo1
Other names/aliases AA420417
Crm1
Exp1
Species Mus musculus
 Database cross references - Xpo1
ExoCarta ExoCarta_103573
Vesiclepedia VP_103573
Entrez Gene 103573
UniProt Q6P5F9  
 Xpo1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Xpo1
Molecular Function
    RNA binding GO:0003723 IEA
    nuclear export signal receptor activity GO:0005049 IBA
    nuclear export signal receptor activity GO:0005049 IDA
    nuclear export signal receptor activity GO:0005049 IMP
    nuclear export signal receptor activity GO:0005049 ISO
    protein binding GO:0005515 IPI
    protein domain specific binding GO:0019904 ISO
    small GTPase binding GO:0031267 IEA
    DNA-binding transcription factor binding GO:0140297 ISO
Biological Process
    ribosomal subunit export from nucleus GO:0000054 ISO
    ribosomal large subunit export from nucleus GO:0000055 IBA
    ribosomal large subunit export from nucleus GO:0000055 IEA
    ribosomal large subunit export from nucleus GO:0000055 ISO
    ribosomal small subunit export from nucleus GO:0000056 IBA
    ribosomal small subunit export from nucleus GO:0000056 IEA
    ribosomal small subunit export from nucleus GO:0000056 ISO
    negative regulation of transcription by RNA polymerase II GO:0000122 ISO
    mRNA export from nucleus GO:0006406 IDA
    mRNA export from nucleus GO:0006406 IMP
    protein export from nucleus GO:0006611 IDA
    protein export from nucleus GO:0006611 IDA
    protein export from nucleus GO:0006611 IMP
    protein export from nucleus GO:0006611 ISO
    protein export from nucleus GO:0006611 ISO
    nucleocytoplasmic transport GO:0006913 ISO
    regulation of centrosome duplication GO:0010824 IMP
    regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032434 IEA
    regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032434 ISO
    protein localization to nucleus GO:0034504 IMP
    regulation of protein catabolic process GO:0042176 IMP
    ribosome biogenesis GO:0042254 ISO
    regulation of protein export from nucleus GO:0046825 IMP
Subcellular Localization
    kinetochore GO:0000776 ISO
    kinetochore GO:0000776 ISS
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    annulate lamellae GO:0005642 IEA
    annulate lamellae GO:0005642 ISO
    nucleoplasm GO:0005654 ISO
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    Cajal body GO:0015030 IEA
    nuclear membrane GO:0031965 IEA
    nuclear membrane GO:0031965 ISO
    protein-containing complex GO:0032991 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    ribonucleoprotein complex GO:1990904 ISO
 Experiment description of studies that identified Xpo1 in exosomes
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Xpo1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ciita  
Co-fractionation Mus musculus
2 PRPF4B 8899
Affinity Capture-MS Homo sapiens
3 FABP3 2170
Affinity Capture-MS Homo sapiens
4 XPO1 7514
Affinity Capture-MS Homo sapiens
5 Cstf2t  
Co-fractionation Mus musculus
6 SCNM1  
Affinity Capture-MS Homo sapiens
7 Cops5 26754
Reconstituted Complex Mus musculus
8 XDH 7498
Affinity Capture-MS Homo sapiens
9 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
10 Kcna3  
Affinity Capture-MS Mus musculus
11 Kctd13  
Affinity Capture-MS Mus musculus
12 Crem  
Affinity Capture-MS Mus musculus
13 NUP98 4928
Affinity Capture-MS Homo sapiens
14 Bach1 12013
Reconstituted Complex Mus musculus
15 ZBTB48  
Affinity Capture-MS Homo sapiens
16 CCNK  
Affinity Capture-MS Homo sapiens
17 Hdac1 433759
Affinity Capture-Western Mus musculus
18 RPS6KB1 6198
Affinity Capture-Western Homo sapiens
19 Fh1 14194
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
20 Unk  
Affinity Capture-RNA Mus musculus
21 Phax  
Co-fractionation Mus musculus
22 GANAB 23193
Affinity Capture-MS Homo sapiens
23 Eed  
Affinity Capture-MS Mus musculus
24 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Xpo1 is involved
PathwayEvidenceSource
Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal IEA Reactome
Amplification of signal from the kinetochores IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle Checkpoints IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Cyclin A/B1/B2 associated events during G2/M transition IEA Reactome
Deactivation of the beta-catenin transactivating complex IEA Reactome
Developmental Biology IEA Reactome
EML4 and NUDC in mitotic spindle formation IEA Reactome
ESR-mediated signaling IEA Reactome
Estrogen-dependent nuclear events downstream of ESR-membrane signaling IEA Reactome
Extra-nuclear estrogen signaling IEA Reactome
G2/M Transition IEA Reactome
Heme signaling IEA Reactome
HuR (ELAVL1) binds and stabilizes mRNA IEA Reactome
M Phase IEA Reactome
MAPK family signaling cascades IEA Reactome
MAPK6/MAPK4 signaling IEA Reactome
Metabolism of RNA IEA Reactome
MITF-M-regulated melanocyte development IEA Reactome
Mitotic Anaphase IEA Reactome
Mitotic G2-G2/M phases IEA Reactome
Mitotic Metaphase and Anaphase IEA Reactome
Mitotic Prometaphase IEA Reactome
Mitotic Spindle Checkpoint IEA Reactome
Regulation of mRNA stability by proteins that bind AU-rich elements IEA Reactome
Resolution of Sister Chromatid Cohesion IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate Formins IEA Reactome
Separation of Sister Chromatids IEA Reactome
Signal Transduction IEA Reactome
Signaling by Nuclear Receptors IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
Signaling by WNT IEA Reactome
TCF dependent signaling in response to WNT IEA Reactome
Transcriptional and post-translational regulation of MITF-M expression and activity IEA Reactome





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