Gene description for RPS6KB1
Gene name ribosomal protein S6 kinase, 70kDa, polypeptide 1
Gene symbol RPS6KB1
Other names/aliases PS6K
S6K
S6K-beta-1
S6K1
STK14A
p70 S6KA
p70(S6K)-alpha
p70-S6K
p70-alpha
Species Homo sapiens
 Database cross references - RPS6KB1
ExoCarta ExoCarta_6198
Vesiclepedia VP_6198
Entrez Gene 6198
HGNC 10436
MIM 608938
UniProt P23443  
 RPS6KB1 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for RPS6KB1
Molecular Function
    protein kinase activity GO:0004672 IDA
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine kinase activity GO:0004674 TAS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein serine/threonine/tyrosine kinase activity GO:0004712 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    PDZ domain binding GO:0030165 IEA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    peptide binding GO:0042277 IEA
    identical protein binding GO:0042802 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    protein phosphatase 2A binding GO:0051721 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    G1/S transition of mitotic cell cycle GO:0000082 IMP
    behavioral fear response GO:0001662 IEA
    skeletal muscle contraction GO:0003009 IEA
    chromatin remodeling GO:0006338 IEA
    apoptotic process GO:0006915 IEA
    signal transduction GO:0007165 TAS
    germ cell development GO:0007281 IEA
    long-term memory GO:0007616 IEA
    response to xenobiotic stimulus GO:0009410 IEA
    response to mechanical stimulus GO:0009612 IEA
    response to toxic substance GO:0009636 IEA
    response to glucose GO:0009749 IEA
    skeletal muscle atrophy GO:0014732 IEA
    response to electrical stimulus involved in regulation of muscle adaptation GO:0014878 IEA
    positive regulation of smooth muscle cell migration GO:0014911 IEA
    cell migration GO:0016477 IEA
    peptidyl-serine phosphorylation GO:0018105 IMP
    response to nutrient levels GO:0031667 IDA
    cellular response to nutrient GO:0031670 IDA
    TOR signaling GO:0031929 IDA
    TOR signaling GO:0031929 IMP
    response to lipopolysaccharide GO:0032496 IEA
    cellular response to insulin stimulus GO:0032869 IBA
    response to testosterone GO:0033574 IEA
    response to glucagon GO:0033762 IEA
    response to tumor necrosis factor GO:0034612 IEA
    TORC1 signaling GO:0038202 IBA
    negative regulation of apoptotic process GO:0043066 IMP
    response to L-leucine GO:0043201 IEA
    long-chain fatty acid import into cell GO:0044539 ISS
    response to ethanol GO:0045471 IEA
    positive regulation of translation GO:0045727 IMP
    positive regulation of mitotic cell cycle GO:0045931 IMP
    positive regulation of translational initiation GO:0045948 IDA
    positive regulation of translational initiation GO:0045948 IMP
    regulation of D-glucose import GO:0046324 IEA
    negative regulation of insulin receptor signaling pathway GO:0046627 IMP
    phosphatidylinositol-mediated signaling GO:0048015 TAS
    positive regulation of skeletal muscle tissue growth GO:0048633 IEA
    positive regulation of smooth muscle cell proliferation GO:0048661 IEA
    modulation of chemical synaptic transmission GO:0050804 IEA
    cellular response to type II interferon GO:0071346 IEA
    cellular response to growth factor stimulus GO:0071363 IDA
    cellular response to dexamethasone stimulus GO:0071549 IEA
    negative regulation of TORC2 signaling GO:1903940 IDA
    positive regulation of TORC1 signaling GO:1904263 IDA
    negative regulation of extrinsic apoptotic signaling pathway GO:2001237 IEA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IBA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 IDA
    mitochondrial outer membrane GO:0005741 IEA
    cytosol GO:0005829 TAS
    cell surface GO:0009986 IEA
    neuron projection GO:0043005 IEA
    perinuclear region of cytoplasm GO:0048471 IEA
    postsynapse GO:0098794 IEA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified RPS6KB1 in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for RPS6KB1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CLEC3B 7123
Two-hybrid Homo sapiens
2 POLDIP3 84271
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
3 UBL4A 8266
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 EIF3C 8663
Affinity Capture-Western Homo sapiens
5 RPA2 6118
Proximity Label-MS Homo sapiens
6 NFATC1 4772
Affinity Capture-Western Homo sapiens
7 CAPZA1 829
Co-fractionation Homo sapiens
8 CSNK2A1 1457
Reconstituted Complex Homo sapiens
9 VHL  
Negative Genetic Homo sapiens
10 PSMD10 5716
Co-fractionation Homo sapiens
11 TRIB2 28951
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
12 GFRA1 2674
Co-fractionation Homo sapiens
13 APP 351
Reconstituted Complex Homo sapiens
14 APEX1 328
Affinity Capture-RNA Homo sapiens
15 DPPA4  
Two-hybrid Homo sapiens
16 NR1I2  
Biochemical Activity Homo sapiens
17 RHEB 6009
Biochemical Activity Homo sapiens
18 NCBP1 4686
Affinity Capture-Western Homo sapiens
19 EIF3B 8662
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
20 IMPDH2 3615
Co-fractionation Homo sapiens
21 SRC 6714
Biochemical Activity Homo sapiens
22 SYK 6850
Biochemical Activity Homo sapiens
23 PLD2 5338
Affinity Capture-Western Homo sapiens
24 TERT  
Affinity Capture-Western Homo sapiens
25 PSAP 5660
Co-fractionation Homo sapiens
26 CSNK2B 1460
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
27 NEDD4 4734
Biochemical Activity Homo sapiens
28 GDA 9615
Affinity Capture-MS Homo sapiens
29 TRAF4 9618
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
30 RBMX 27316
Co-fractionation Homo sapiens
31 RB1 5925
Negative Genetic Homo sapiens
32 PDK1 5163
Reconstituted Complex Homo sapiens
33 RCC2 55920
Co-fractionation Homo sapiens
34 KIT 3815
Negative Genetic Homo sapiens
35 COASY 80347
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
36 PPP5C 5536
Affinity Capture-MS Homo sapiens
37 RBX1 9978
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
38 LYN 4067
Biochemical Activity Homo sapiens
39 CCT5 22948
Co-fractionation Homo sapiens
40 HTT 3064
Protein-RNA Homo sapiens
41 RPS6KB1 6198
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
42 MAPT  
Biochemical Activity Homo sapiens
43 UBA3 9039
Co-fractionation Homo sapiens
44 DDX5 1655
Co-fractionation Homo sapiens
45 BICD2 23299
Proximity Label-MS Homo sapiens
46 BTK 695
Biochemical Activity Homo sapiens
47 MAPK8 5599
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
48 RNF40 9810
Co-fractionation Homo sapiens
49 TIPRL 261726
Co-fractionation Homo sapiens
50 XPO1 7514
Affinity Capture-Western Homo sapiens
51 PRKCZ 5590
Affinity Capture-Western Homo sapiens
52 RICTOR 253260
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
53 MXD1  
Biochemical Activity Homo sapiens
54 EIF4E 1977
Affinity Capture-Western Homo sapiens
55 PDPK1 5170
Affinity Capture-Western Homo sapiens
56 Xpo1 103573
Affinity Capture-Western Mus musculus
57 PIP4K2A 5305
Biochemical Activity Homo sapiens
58 RPA3 6119
Proximity Label-MS Homo sapiens
59 DYNC1LI1 51143
Proximity Label-MS Homo sapiens
60 MTOR 2475
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
61 MAPKAP1 79109
Co-fractionation Homo sapiens
62 GDI2P1  
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
63 BICD1 636
Proximity Label-MS Homo sapiens
64 AKT1 207
Co-localization Homo sapiens
Co-purification Homo sapiens
65 EEF2K  
Reconstituted Complex Homo sapiens
66 MDM2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
67 NAA10 8260
Co-fractionation Homo sapiens
68 EIF3H 8667
Affinity Capture-MS Homo sapiens
69 KIR3DL1  
Two-hybrid Homo sapiens
70 EIF3F 8665
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
71 RRM2 6241
Negative Genetic Homo sapiens
72 DEPTOR 64798
Biochemical Activity Homo sapiens
73 JUP 3728
Co-fractionation Homo sapiens
74 MCM5 4174
Co-fractionation Homo sapiens
75 KAT2B  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
76 RPS12 6206
Co-fractionation Homo sapiens
77 RPTOR 57521
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
78 SQSTM1 8878
Affinity Capture-Western Homo sapiens
79 ABL1 25
Negative Genetic Homo sapiens
80 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
81 RNF20 56254
Co-fractionation Homo sapiens
82 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
83 GTF2I 2969
Co-fractionation Homo sapiens
84 TUBB4A 10382
Affinity Capture-MS Homo sapiens
85 RAD50 10111
Co-fractionation Homo sapiens
86 ACTA1 58
Reconstituted Complex Homo sapiens
87 RPS6 6194
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
88 STK11 6794
Co-localization Homo sapiens
89 HSPA4L 22824
Co-fractionation Homo sapiens
90 PPP2R2A 5520
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
91 IRS1 3667
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
92 EP300 2033
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
93 NAE1 8883
Co-fractionation Homo sapiens
94 PARN  
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which RPS6KB1 is involved
PathwayEvidenceSource
MTOR signalling TAS Reactome
mTORC1-mediated signalling TAS Reactome
Signal Transduction TAS Reactome





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