Gene description for POLDIP3
Gene name polymerase (DNA-directed), delta interacting protein 3
Gene symbol POLDIP3
Other names/aliases PDIP46
SKAR
Species Homo sapiens
 Database cross references - POLDIP3
ExoCarta ExoCarta_84271
Vesiclepedia VP_84271
Entrez Gene 84271
HGNC 23782
MIM 611520
UniProt Q9BY77  
 POLDIP3 identified in exosomes derived from the following tissue/cell type
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
 Gene ontology annotations for POLDIP3
Molecular Function
    RNA binding GO:0003723 HDA
    mRNA binding GO:0003729 IBA
    protein binding GO:0005515 IPI
    protein-containing complex binding GO:0044877 IPI
Biological Process
    poly(A)+ mRNA export from nucleus GO:0016973 IBA
    poly(A)+ mRNA export from nucleus GO:0016973 IDA
    positive regulation of translation GO:0045727 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 TAS
    cytosol GO:0005829 TAS
    nuclear speck GO:0016607 IBA
    nuclear speck GO:0016607 IDA
    cytoplasmic ribonucleoprotein granule GO:0036464 IDA
 Experiment description of studies that identified POLDIP3 in exosomes
1
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for POLDIP3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RBPMS 11030
Two-hybrid Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 SNRPA 6626
Affinity Capture-MS Homo sapiens
4 LUZP4  
Affinity Capture-MS Homo sapiens
5 SENP3 26168
Affinity Capture-MS Homo sapiens
6 NCBP1 4686
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
7 KPNA3 3839
Affinity Capture-MS Homo sapiens
8 BRDT  
Affinity Capture-MS Homo sapiens
9 MAGEA3  
Affinity Capture-MS Homo sapiens
10 POT1  
Affinity Capture-MS Homo sapiens
11 BCLAF1 9774
Affinity Capture-MS Homo sapiens
12 TPX2  
Affinity Capture-MS Homo sapiens
13 TLX3  
Affinity Capture-MS Homo sapiens
14 EPB41L5 57669
Affinity Capture-MS Homo sapiens
15 Chmp4b 75608
Affinity Capture-MS Mus musculus
16 DHX8 1659
Proximity Label-MS Homo sapiens
17 BRD2  
Affinity Capture-MS Homo sapiens
18 THOC7 80145
Affinity Capture-MS Homo sapiens
19 KDM4A  
Affinity Capture-MS Homo sapiens
20 RPS6KB2  
Affinity Capture-MS Homo sapiens
21 SOX2  
Affinity Capture-MS Homo sapiens
22 APEX1 328
Affinity Capture-RNA Homo sapiens
23 PSIP1 11168
Affinity Capture-MS Homo sapiens
24 DDX23 9416
Proximity Label-MS Homo sapiens
25 FERMT3 83706
Affinity Capture-MS Homo sapiens
26 PFKFB2 5208
Affinity Capture-MS Homo sapiens
27 KRT40  
Two-hybrid Homo sapiens
28 FBXW7  
Affinity Capture-MS Homo sapiens
29 PRC1 9055
Affinity Capture-MS Homo sapiens
30 C8orf82  
Proximity Label-MS Homo sapiens
31 UFL1 23376
Affinity Capture-MS Homo sapiens
32 ETV4  
Proximity Label-MS Homo sapiens
33 OBSL1 23363
Affinity Capture-MS Homo sapiens
34 MTUS2 23281
Two-hybrid Homo sapiens
35 TEKT2  
Affinity Capture-MS Homo sapiens
36 SARNP 84324
Affinity Capture-MS Homo sapiens
37 KLHL20  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 MECP2 4204
Affinity Capture-MS Homo sapiens
39 MYCN  
Affinity Capture-MS Homo sapiens
40 THOC1 9984
Affinity Capture-MS Homo sapiens
41 SOX5 6660
Affinity Capture-MS Homo sapiens
42 Erh  
Affinity Capture-MS Mus musculus
43 OCLM  
Affinity Capture-MS Homo sapiens
44 PSME3 10197
Affinity Capture-MS Homo sapiens
45 BANP  
Two-hybrid Homo sapiens
46 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
47 TOP3B 8940
Affinity Capture-MS Homo sapiens
48 SOX17  
Proximity Label-MS Homo sapiens
49 CYB5D2  
Affinity Capture-MS Homo sapiens
50 C17orf85  
Proximity Label-MS Homo sapiens
51 ELK3  
Proximity Label-MS Homo sapiens
52 PPP1R16B  
Affinity Capture-MS Homo sapiens
53 GOLGA2 2801
Two-hybrid Homo sapiens
54 RPS6KB1 6198
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
55 UBR5 51366
Affinity Capture-MS Homo sapiens
56 OGT 8473
Reconstituted Complex Homo sapiens
57 KLF5  
Affinity Capture-MS Homo sapiens
58 U2AF2 11338
Affinity Capture-MS Homo sapiens
59 S100B 6285
Affinity Capture-MS Homo sapiens
60 EIF4G2 1982
Co-fractionation Homo sapiens
61 HECTD1 25831
Affinity Capture-MS Homo sapiens
62 THOC2 57187
Affinity Capture-MS Homo sapiens
63 DDX39B 7919
Affinity Capture-MS Homo sapiens
64 H2AFY 9555
Affinity Capture-MS Homo sapiens
65 CEBPA  
Protein-peptide Homo sapiens
66 NFATC4 4776
Proximity Label-MS Homo sapiens
67 RPS6KA1 6195
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
68 YEATS4  
Affinity Capture-MS Homo sapiens
69 MYH7B  
Affinity Capture-MS Homo sapiens
70 TLX2  
Affinity Capture-MS Homo sapiens
71 Bbs5  
Two-hybrid Mus musculus
72 PRPF4B 8899
Affinity Capture-MS Homo sapiens
73 SOX6  
Affinity Capture-MS Homo sapiens
74 RC3H2  
Affinity Capture-MS Homo sapiens
75 SIRT7  
Affinity Capture-MS Homo sapiens
76 SNRPB 6628
Affinity Capture-MS Homo sapiens
77 CTNNB1 1499
Affinity Capture-MS Homo sapiens
78 CUL3 8452
Affinity Capture-MS Homo sapiens
79 MAB21L2  
Affinity Capture-MS Homo sapiens
80 THOC5 8563
Affinity Capture-MS Homo sapiens
81 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
82 RBM33  
Affinity Capture-MS Homo sapiens
83 Max  
Affinity Capture-MS Mus musculus
84 A2ML1 144568
Affinity Capture-MS Homo sapiens
85 LZTS2 84445
Two-hybrid Homo sapiens
86 UBR1 197131
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 MYC  
Affinity Capture-MS Homo sapiens
88 RPA3 6119
Proximity Label-MS Homo sapiens
89 DDRGK1 65992
Affinity Capture-MS Homo sapiens
90 RNF2  
Affinity Capture-MS Homo sapiens
91 Dynlt1b  
Two-hybrid Mus musculus
92 SNRPF 6636
Two-hybrid Homo sapiens
93 CCDC8  
Affinity Capture-MS Homo sapiens
94 STAT4  
Proximity Label-MS Homo sapiens
95 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
96 USE1 55850
Affinity Capture-MS Homo sapiens
97 TERF2  
Affinity Capture-MS Homo sapiens
98 NXF1 10482
Affinity Capture-RNA Homo sapiens
99 HDGF 3068
Affinity Capture-MS Homo sapiens
100 ERH 2079
Two-hybrid Homo sapiens
101 FOSL1  
Affinity Capture-MS Homo sapiens
102 ZNF746  
Affinity Capture-MS Homo sapiens
103 EIF4G1 1981
Affinity Capture-Western Homo sapiens
104 PDHA1 5160
Affinity Capture-MS Homo sapiens
105 SOX4  
Proximity Label-MS Homo sapiens
106 FYCO1 79443
Affinity Capture-MS Homo sapiens
107 PNMA1 9240
Two-hybrid Homo sapiens
108 EIF4A3 9775
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
109 EZH1  
Affinity Capture-MS Homo sapiens
110 DHX40  
Proximity Label-MS Homo sapiens
111 RNASEH2A 10535
Affinity Capture-MS Homo sapiens
112 Srsf1 110809
Affinity Capture-MS Mus musculus
113 NQO1 1728
Co-fractionation Homo sapiens
114 MED12  
Affinity Capture-MS Homo sapiens
115 MOV10 4343
Affinity Capture-RNA Homo sapiens
116 RTCB 51493
Affinity Capture-MS Homo sapiens
117 CIT 11113
Affinity Capture-MS Homo sapiens
118 TOLLIP 54472
Affinity Capture-MS Homo sapiens
119 KLHL22 84861
Affinity Capture-MS Homo sapiens
120 EXOSC8  
Two-hybrid Homo sapiens
121 CUL7 9820
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
122 ATXN3 4287
Affinity Capture-MS Homo sapiens
123 PRMT1 3276
Affinity Capture-MS Homo sapiens
124 USP20  
Affinity Capture-MS Homo sapiens
125 EP300 2033
Affinity Capture-MS Homo sapiens
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