Gene description for UBR5
Gene name ubiquitin protein ligase E3 component n-recognin 5
Gene symbol UBR5
Other names/aliases DD5
EDD
EDD1
HYD
Species Homo sapiens
 Database cross references - UBR5
ExoCarta ExoCarta_51366
Vesiclepedia VP_51366
Entrez Gene 51366
HGNC 16806
MIM 608413
UniProt O95071  
 UBR5 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for UBR5
Molecular Function
    RNA binding GO:0003723 IEA
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
    ubiquitin-ubiquitin ligase activity GO:0034450 IBA
    ubiquitin-ubiquitin ligase activity GO:0034450 IDA
    ubiquitin binding GO:0043130 IDA
    ubiquitin protein ligase activity GO:0061630 IDA
Biological Process
    protein polyubiquitination GO:0000209 IBA
    protein polyubiquitination GO:0000209 IDA
    DNA repair GO:0006281 IEA
    DNA damage response GO:0006974 IDA
    positive regulation of gene expression GO:0010628 IDA
    heterochromatin boundary formation GO:0033696 IMP
    protein K29-linked ubiquitination GO:0035519 IDA
    positive regulation of protein import into nucleus GO:0042307 IMP
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IDA
    negative regulation of smoothened signaling pathway GO:0045879 ISS
    progesterone receptor signaling pathway GO:0050847 IDA
    protein K48-linked ubiquitination GO:0070936 IDA
    protein K11-linked ubiquitination GO:0070979 IDA
    cytoplasm protein quality control by the ubiquitin-proteasome system GO:0071629 IDA
    nuclear protein quality control by the ubiquitin-proteasome system GO:0071630 IDA
    positive regulation of canonical Wnt signaling pathway GO:0090263 IBA
    positive regulation of canonical Wnt signaling pathway GO:0090263 IMP
    cytoplasm protein quality control GO:0140455 IDA
    DNA repair-dependent chromatin remodeling GO:0140861 IMP
    protein branched polyubiquitination GO:0141198 IDA
Subcellular Localization
    chromatin GO:0000785 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    membrane GO:0016020 HDA
    protein-containing complex GO:0032991 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
 Experiment description of studies that identified UBR5 in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for UBR5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DSN1 79980
Affinity Capture-MS Homo sapiens
2 MCRS1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
3 UBE2H 7328
Affinity Capture-MS Homo sapiens
4 OTUD5 55593
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
5 AFF4 27125
Co-fractionation Homo sapiens
6 HOOK1  
Proximity Label-MS Homo sapiens
7 SPRTN  
Affinity Capture-MS Homo sapiens
8 UBE2A  
Reconstituted Complex Homo sapiens
9 EEF1G 1937
Co-fractionation Homo sapiens
10 PMS1  
Affinity Capture-Western Homo sapiens
11 KPNA1 3836
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
12 BUB1  
Affinity Capture-MS Homo sapiens
13 MSH2 4436
Co-fractionation Homo sapiens
14 WNK1 65125
Affinity Capture-Western Homo sapiens
15 ZBED6CL  
Affinity Capture-MS Homo sapiens
16 TPD52L1 7164
Co-fractionation Homo sapiens
17 MOAP1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
18 VPRBP 9730
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
19 PDCD1  
Affinity Capture-MS Homo sapiens
20 SUB1 10923
Affinity Capture-MS Homo sapiens
21 EBNA-LP  
Affinity Capture-MS
22 DBN1 1627
Affinity Capture-MS Homo sapiens
23 BUB1B  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
24 UBC 7316
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
25 CCT4 10575
Affinity Capture-MS Homo sapiens
26 CHEK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
27 CALM1 801
Affinity Capture-Western Homo sapiens
28 VHL  
Affinity Capture-MS Homo sapiens
29 ANKRD28 23243
Proximity Label-MS Homo sapiens
30 STX11 8676
Affinity Capture-MS Homo sapiens
31 EEF1A2 1917
Affinity Capture-MS Homo sapiens
32 ZNF207 7756
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
33 TCEA1 6917
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
34 KIAA1429 25962
Affinity Capture-MS Homo sapiens
35 DPPA4  
Affinity Capture-MS Homo sapiens
36 WTAP 9589
Affinity Capture-MS Homo sapiens
37 PPL 5493
Affinity Capture-MS Homo sapiens
38 NR1I2  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
39 WDR48 57599
Affinity Capture-MS Homo sapiens
40 PGK1 5230
Affinity Capture-MS Homo sapiens
41 PFKL 5211
Co-fractionation Homo sapiens
42 TNRC6A 27327
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
43 GTF2E2  
Affinity Capture-MS Homo sapiens
44 KIF23 9493
Affinity Capture-MS Homo sapiens
45 PCK1 5105
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
46 DYRK2 8445
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
47 GKN1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
48 H2AFV 94239
Affinity Capture-MS Homo sapiens
49 TOB1  
Affinity Capture-Western Homo sapiens
50 ACTR1A 10121
Co-fractionation Homo sapiens
51 F9 2158
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 POU5F1  
Affinity Capture-MS Homo sapiens
53 MARCH5  
Affinity Capture-MS Homo sapiens
54 PPEF1  
Affinity Capture-MS Homo sapiens
55 PSMC2 5701
Co-fractionation Homo sapiens
56 Cenpe  
Affinity Capture-MS Mus musculus
57 HNRNPU 3192
Co-fractionation Homo sapiens
58 MMP19 4327
Affinity Capture-MS Homo sapiens
59 MRS2  
Affinity Capture-MS Homo sapiens
60 DNAJC16  
Affinity Capture-MS Homo sapiens
61 POLDIP3 84271
Affinity Capture-MS Homo sapiens
62 Ubr5  
Affinity Capture-MS Mus musculus
63 ASH2L 9070
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 PMS2  
Affinity Capture-MS Homo sapiens
65 APC  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
66 ARPC1B 10095
Affinity Capture-MS Homo sapiens
67 Mcm3 17215
Affinity Capture-MS Mus musculus
68 CUL1 8454
Affinity Capture-MS Homo sapiens
69 HUWE1 10075
Affinity Capture-MS Homo sapiens
70 MRPL4 51073
Affinity Capture-MS Homo sapiens
71 WDR5 11091
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
72 FAF2 23197
Affinity Capture-MS Homo sapiens
73 TOB2  
Affinity Capture-Western Homo sapiens
74 MAD2L1 4085
Affinity Capture-Western Homo sapiens
75 FBXW5  
Affinity Capture-MS Homo sapiens
76 EEF1A1 1915
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
77 GFI1B  
Affinity Capture-MS Homo sapiens
78 GSK3B 2932
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
79 KIF14 9928
Affinity Capture-MS Homo sapiens
80 DDX58 23586
Affinity Capture-RNA Homo sapiens
81 NCOA1  
Affinity Capture-MS Homo sapiens
82 AGO2 27161
Affinity Capture-Western Homo sapiens
83 MAPK1 5594
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
84 DDX23 9416
Co-fractionation Homo sapiens
85 XPO1 7514
Affinity Capture-MS Homo sapiens
86 GEMIN5 25929
Affinity Capture-MS Homo sapiens
87 PPP1CA 5499
Reconstituted Complex Homo sapiens
88 RPS9 6203
Affinity Capture-MS Homo sapiens
89 NOLC1 9221
Co-fractionation Homo sapiens
90 BICD1 636
Proximity Label-MS Homo sapiens
91 TCP1 6950
Affinity Capture-MS Homo sapiens
92 MAPK3 5595
Affinity Capture-Western Homo sapiens
93 PRPF8 10594
Co-fractionation Homo sapiens
94 CAPZA1 829
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
95 GSPT1 2935
Affinity Capture-Western Homo sapiens
96 CTDP1 9150
Affinity Capture-MS Homo sapiens
97 CDKN1A  
Affinity Capture-MS Homo sapiens
98 UTP15 84135
Co-fractionation Homo sapiens
99 SUPT5H 6829
Affinity Capture-MS Homo sapiens
100 NONO 4841
Affinity Capture-MS Homo sapiens
101 UBAP2L 9898
Affinity Capture-MS Homo sapiens
102 BAG6 7917
Affinity Capture-MS Homo sapiens
103 SNRNP200 23020
Co-fractionation Homo sapiens
104 TULP3 7289
Affinity Capture-MS Homo sapiens
105 UBR5 51366
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
106 PABPC1 26986
Co-crystal Structure Homo sapiens
107 CHMP4B 128866
Affinity Capture-MS Homo sapiens
108 SREBF2 6721
Positive Genetic Homo sapiens
109 BARD1 580
Two-hybrid Homo sapiens
110 RNF168  
Affinity Capture-Western Homo sapiens
111 SLX4  
Affinity Capture-MS Homo sapiens
112 PTK2B 2185
Affinity Capture-Western Homo sapiens
113 FBXL6  
Affinity Capture-MS Homo sapiens
114 PNMA2  
Affinity Capture-MS Homo sapiens
115 SMAD2 4087
Two-hybrid Homo sapiens
116 CIB1 10519
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
117 DSP 1832
Affinity Capture-MS Homo sapiens
118 CRY2  
Affinity Capture-MS Homo sapiens
119 PTRF 284119
Co-fractionation Homo sapiens
120 C19orf48  
Affinity Capture-MS Homo sapiens
121 UBE2D3 7323
Reconstituted Complex Homo sapiens
122 C1orf115  
Affinity Capture-MS Homo sapiens
123 IGFL3  
Affinity Capture-MS Homo sapiens
124 DNAJC30  
Affinity Capture-MS Homo sapiens
125 Bub1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
126 UBE2B 7320
Reconstituted Complex Homo sapiens
127 RPS15A 6210
Affinity Capture-MS Homo sapiens
128 CEBPA  
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
129 GAR1 54433
Affinity Capture-MS Homo sapiens
130 MYH4  
Affinity Capture-MS Homo sapiens
131 RPS6KA1 6195
Affinity Capture-Western Homo sapiens
132 VCP 7415
Affinity Capture-MS Homo sapiens
133 IGBP1 3476
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
134 TXN2 25828
Affinity Capture-Western Homo sapiens
135 HNRNPLL 92906
Affinity Capture-MS Homo sapiens
136 RING1 6015
Co-localization Homo sapiens
137 PHB 5245
Affinity Capture-MS Homo sapiens
138 RECQL5  
Affinity Capture-MS Homo sapiens
139 ACTN1 87
Affinity Capture-MS Homo sapiens
140 HSPA5 3309
Co-fractionation Homo sapiens
141 TUBB 203068
Co-fractionation Homo sapiens
142 RRP1B 23076
Affinity Capture-MS Homo sapiens
143 TERT  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
144 FOXG1  
Affinity Capture-MS Homo sapiens
145 CDC73  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
146 RPS2 6187
Affinity Capture-MS Homo sapiens
147 AMBRA1  
Affinity Capture-MS Homo sapiens
148 CALR3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
149 SIRT7  
Affinity Capture-MS Homo sapiens
150 UBXN7 26043
Affinity Capture-MS Homo sapiens
151 UQCRC2 7385
Co-fractionation Homo sapiens
152 UBE2L3 7332
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
153 AURKA 6790
Affinity Capture-MS Homo sapiens
154 SRSF1 6426
Co-fractionation Homo sapiens
155 RPL14 9045
Affinity Capture-MS Homo sapiens
156 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
157 DUSP26 78986
Affinity Capture-MS Homo sapiens
158 XPNPEP3 63929
Affinity Capture-MS Homo sapiens
159 LYZL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
160 C16orf72 29035
Affinity Capture-MS Homo sapiens
161 KIAA1524 57650
Two-hybrid Homo sapiens
162 METTL3  
Affinity Capture-MS Homo sapiens
163 PGR  
Reconstituted Complex Homo sapiens
164 ATMIN  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
165 SPTBN1 6711
Affinity Capture-MS Homo sapiens
166 WDR43 23160
Co-fractionation Homo sapiens
167 AURKB 9212
Affinity Capture-MS Homo sapiens
168 EFTUD2 9343
Co-fractionation Homo sapiens
169 SLC25A11 8402
Affinity Capture-MS Homo sapiens
170 UBE2D2 7322
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
171 MAPK6  
Affinity Capture-MS Homo sapiens
172 Igbp1 58845
Two-hybrid Rattus norvegicus
173 MYC  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
174 ZUFSP 221302
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
175 NLRP3  
Affinity Capture-MS Homo sapiens
176 SET 6418
Co-fractionation Homo sapiens
177 PRMT2  
Affinity Capture-MS Homo sapiens
178 Cep78  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
179 ACTN4 81
Affinity Capture-MS Homo sapiens
180 TOLLIP 54472
Proximity Label-MS Homo sapiens
181 UBE2O 63893
Co-fractionation Homo sapiens
182 SERINC5 256987
Affinity Capture-MS Homo sapiens
183 DDB1 1642
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
184 PPAN 56342
Affinity Capture-MS Homo sapiens
185 Bub1b 12236
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
186 RPS11 6205
Affinity Capture-MS Homo sapiens
187 RNF2  
Co-localization Homo sapiens
188 BMI1  
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
189 XRCC3  
Affinity Capture-MS Homo sapiens
190 P4HA3 283208
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
191 POGLUT1 56983
Affinity Capture-MS Homo sapiens
192 PML 5371
Affinity Capture-MS Homo sapiens
193 Ostm1  
Affinity Capture-MS Mus musculus
Reconstituted Complex Mus musculus
194 SUPT16H 11198
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
195 UBXN1 51035
Affinity Capture-MS Homo sapiens
196 CCDC8  
Affinity Capture-MS Homo sapiens
197 ACTBL2 345651
Affinity Capture-MS Homo sapiens
198 SATB1 6304
Two-hybrid Homo sapiens
199 UBE2D1 7321
Co-fractionation Homo sapiens
200 PAIP1 10605
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
201 NES 10763
Affinity Capture-MS Homo sapiens
202 DNAJC28  
Affinity Capture-MS Homo sapiens
203 KIF2A 3796
Affinity Capture-MS Homo sapiens
204 ARRB2 409
Affinity Capture-MS Homo sapiens
205 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
206 FBXW11  
Affinity Capture-Western Homo sapiens
207 SPTAN1 6709
Affinity Capture-MS Homo sapiens
208 CDC20 991
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
209 SIRT6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
210 MYL6 4637
Affinity Capture-MS Homo sapiens
211 PDGFD 80310
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
212 USP49 25862
Affinity Capture-MS Homo sapiens
213 TOPBP1 11073
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
214 TP53 7157
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
215 KIF20A 10112
Affinity Capture-MS Homo sapiens
216 TUBG1 7283
Affinity Capture-MS Homo sapiens
217 EIF3I 8668
Co-fractionation Homo sapiens
218 RUVBL2 10856
Affinity Capture-MS Homo sapiens
219 FOXJ2  
Affinity Capture-MS Homo sapiens
220 ZNF746  
Affinity Capture-MS Homo sapiens
221 FOXB1  
Affinity Capture-MS Homo sapiens
222 MEN1 4221
Affinity Capture-MS Homo sapiens
223 TUBGCP3 10426
Affinity Capture-MS Homo sapiens
224 NCOA2 10499