Gene description for DYRK2
Gene name dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2
Gene symbol DYRK2
Other names/aliases -
Species Homo sapiens
 Database cross references - DYRK2
ExoCarta ExoCarta_8445
Vesiclepedia VP_8445
Entrez Gene 8445
HGNC 3093
MIM 603496
UniProt Q92630  
 DYRK2 identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for DYRK2
Molecular Function
    magnesium ion binding GO:0000287 IDA
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine kinase activity GO:0004674 TAS
    protein serine/threonine/tyrosine kinase activity GO:0004712 IEA
    protein tyrosine kinase activity GO:0004713 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    manganese ion binding GO:0030145 IDA
    histone H3Y41 kinase activity GO:0035401 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2AXY142 kinase activity GO:0140801 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 IDA
    DNA damage response GO:0006974 IEP
    smoothened signaling pathway GO:0007224 IMP
    intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator GO:0042771 IDA
    positive regulation of glycogen biosynthetic process GO:0045725 IDA
    negative regulation of calcineurin-NFAT signaling cascade GO:0070885 IMP
    regulation of signal transduction by p53 class mediator GO:1901796 TAS
Subcellular Localization
    ubiquitin ligase complex GO:0000151 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    cytoskeleton GO:0005856 IBA
    ribonucleoprotein complex GO:1990904 IEA
 Experiment description of studies that identified DYRK2 in exosomes
1
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for DYRK2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TUBA3C 7278
Affinity Capture-MS Homo sapiens
2 KIF3B 9371
Affinity Capture-MS Homo sapiens
3 TERT  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
4 HSPA6 3310
Affinity Capture-MS Homo sapiens
5 HSPA5 3309
Affinity Capture-MS Homo sapiens
6 MAPK4  
Affinity Capture-MS Homo sapiens
7 DAP3 7818
Affinity Capture-MS Homo sapiens
8 FTL 2512
Affinity Capture-MS Homo sapiens
9 SLC25A3 5250
Affinity Capture-MS Homo sapiens
10 SIAH2 6478
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
11 TP53 7157
Negative Genetic Homo sapiens
12 RPS2 6187
Affinity Capture-MS Homo sapiens
13 HSPA8 3312
Affinity Capture-MS Homo sapiens
14 DYRK4  
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
Affinity Capture-MS Homo sapiens
15 AKAP12 9590
Affinity Capture-MS Homo sapiens
16 RBM47 54502
Affinity Capture-MS Homo sapiens
17 PCMT1 5110
Affinity Capture-MS Homo sapiens
18 MOAP1  
Affinity Capture-Western Homo sapiens
19 VPRBP 9730
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
20 RPA1 6117
Affinity Capture-MS Homo sapiens
21 SAV1  
Affinity Capture-MS Homo sapiens
22 XPNPEP3 63929
Affinity Capture-MS Homo sapiens
23 LZTS2 84445
Two-hybrid Homo sapiens
24 EGFR 1956
Negative Genetic Homo sapiens
25 PDHB 5162
Affinity Capture-MS Homo sapiens
26 RB1CC1 9821
Affinity Capture-MS Homo sapiens
27 RPL4 6124
Affinity Capture-MS Homo sapiens
28 KATNA1 11104
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
29 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
30 SSBP2  
Affinity Capture-MS Homo sapiens
31 IKZF1  
Two-hybrid Homo sapiens
32 RPL7 6129
Affinity Capture-MS Homo sapiens
33 DCAF5  
Affinity Capture-MS Homo sapiens
34 APP 351
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
35 POTEF 728378
Affinity Capture-MS Homo sapiens
36 TBK1 29110
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
37 NR1I2  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
38 GSK3B 2932
Affinity Capture-Western Homo sapiens
39 UBR5 51366
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
40 EEF1A1P5 158078
Affinity Capture-MS Homo sapiens
41 ATM 472
Biochemical Activity Homo sapiens
42 RPL7A 6130
Affinity Capture-MS Homo sapiens
43 WDR62  
Two-hybrid Homo sapiens
44 RBMXL2  
Affinity Capture-MS Homo sapiens
45 ZBTB9  
Two-hybrid Homo sapiens
46 RAD54B  
Two-hybrid Homo sapiens
47 DCAF7 10238
Affinity Capture-Western Homo sapiens
48 DLST 1743
Affinity Capture-MS Homo sapiens
49 RNF8  
Affinity Capture-Western Homo sapiens
50 KXD1 79036
Two-hybrid Homo sapiens
51 HSPA1A 3303
Affinity Capture-MS Homo sapiens
52 TRIOBP 11078
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
53 TTN 7273
Affinity Capture-MS Homo sapiens
54 NF2 4771
Negative Genetic Homo sapiens
55 DDB1 1642
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
56 RPL18 6141
Affinity Capture-MS Homo sapiens
57 TUBA1C 84790
Affinity Capture-MS Homo sapiens
58 HNRNPU 3192
Affinity Capture-MS Homo sapiens
59 NPM1 4869
Affinity Capture-MS Homo sapiens
60 STK4 6789
Affinity Capture-MS Homo sapiens
61 EPS8L1 54869
Affinity Capture-MS Homo sapiens
62 RPLP0 6175
Affinity Capture-MS Homo sapiens
63 KIF3C 3797
Affinity Capture-MS Homo sapiens
64 RTN1 6252
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 UBE2D1 7321
Reconstituted Complex Homo sapiens
66 HSPA1B 3304
Affinity Capture-MS Homo sapiens
67 MDM2  
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
68 SP100 6672
Two-hybrid Homo sapiens
View the network image/svg+xml



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