Gene description for VPRBP
Gene name Vpr (HIV-1) binding protein
Gene symbol VPRBP
Other names/aliases DCAF1
Species Homo sapiens
 Database cross references - VPRBP
ExoCarta ExoCarta_9730
Vesiclepedia VP_9730
Entrez Gene 9730
HGNC 30911
UniProt Q9Y4B6  
 VPRBP identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for VPRBP
Molecular Function
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    nuclear estrogen receptor binding GO:0030331 IBA
    nuclear estrogen receptor binding GO:0030331 IPI
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IBA
    histone H2AT120 kinase activity GO:1990244 IDA
    ubiquitin-like ligase-substrate adaptor activity GO:1990756 IDA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    chromatin remodeling GO:0006338 IEA
    protein ubiquitination GO:0016567 IEA
    B cell differentiation GO:0030183 ISS
    V(D)J recombination GO:0033151 ISS
    cell competition in a multicellular organism GO:0035212 IMP
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IDA
    post-translational protein modification GO:0043687 IDA
    positive regulation of protein catabolic process GO:0045732 IDA
Subcellular Localization
    fibrillar center GO:0001650 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IEA
    centrosome GO:0005813 IDA
    COP9 signalosome GO:0008180 IDA
    Cul4-RING E3 ubiquitin ligase complex GO:0080008 IBA
    Cul4-RING E3 ubiquitin ligase complex GO:0080008 IDA
 Experiment description of studies that identified VPRBP in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for VPRBP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CFAP20 29105
Affinity Capture-MS Homo sapiens
2 ATR  
Biochemical Activity Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 SENP3 26168
Affinity Capture-MS Homo sapiens
5 Dclre1b  
Affinity Capture-MS Mus musculus
6 HDAC2 3066
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
7 ANP32A 8125
Co-fractionation Homo sapiens
8 HSPA6 3310
Co-fractionation Homo sapiens
9 WWP1 11059
Affinity Capture-MS Homo sapiens
10 KIFC3 3801
Affinity Capture-MS Homo sapiens
11 Tet3  
Affinity Capture-Western Mus musculus
Biochemical Activity Mus musculus
12 MOAP1  
Affinity Capture-Western Homo sapiens
13 VPRBP 9730
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
14 RPL13A 23521
Affinity Capture-MS Homo sapiens
15 CUL3 8452
Affinity Capture-MS Homo sapiens
16 EPS15 2060
Affinity Capture-MS Homo sapiens
17 RPL10 6134
Affinity Capture-MS Homo sapiens
18 RPL15 6138
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 RPL14 9045
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 ATM 472
Biochemical Activity Homo sapiens
21 PRKDC 5591
Biochemical Activity Homo sapiens
22 KIAA1429 25962
Affinity Capture-MS Homo sapiens
23 UBC 7316
Affinity Capture-Western Homo sapiens
24 CAPZB 832
Affinity Capture-MS Homo sapiens
25 RPS6 6194
Affinity Capture-MS Homo sapiens
26 HDAC3 8841
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
27 PPP2R1B 5519
Affinity Capture-Western Homo sapiens
28 DDA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
29 SMG9 56006
Affinity Capture-MS Homo sapiens
30 SULF2 55959
Affinity Capture-MS Homo sapiens
31 MYH6 4624
Affinity Capture-MS Homo sapiens
32 DYRK2 8445
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
33 COPS6 10980
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 PNKP 11284
Affinity Capture-MS Homo sapiens
35 NR2C2  
Affinity Capture-Western Homo sapiens
36 COPS5 10987
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 POU5F1  
Affinity Capture-MS Homo sapiens
38 TP53TG5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 MAPK14 1432
Affinity Capture-MS Homo sapiens
40 RPS9 6203
Affinity Capture-MS Homo sapiens
41 PLCG1 5335
Co-fractionation Homo sapiens
42 COPS7A 50813
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 TCEANC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 DNAJC16  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
45 U2AF1 7307
Co-fractionation Homo sapiens
46 TERT  
Affinity Capture-Western Homo sapiens
47 KSR1  
Affinity Capture-Western Homo sapiens
48 LATS1  
Affinity Capture-Western Homo sapiens
49 C2CD4B  
Affinity Capture-MS Homo sapiens
50 CUL1 8454
Affinity Capture-Western Homo sapiens
51 GPS1 2873
Affinity Capture-MS Homo sapiens
52 NEDD8 4738
Affinity Capture-MS Homo sapiens
53 ZNF462  
Affinity Capture-MS Homo sapiens
54 RPL19 6143
Affinity Capture-MS Homo sapiens
55 BCL11B  
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
FRET Homo sapiens
56 SAMHD1 25939
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
57 VTN 7448
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 EPB41L3 23136
Affinity Capture-MS Homo sapiens
59 NF2 4771
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
60 ZNF550  
Affinity Capture-MS Homo sapiens
61 ATP5A1 498
Co-fractionation Homo sapiens
62 RBX1 9978
Reconstituted Complex Homo sapiens
63 SRP14 6727
Affinity Capture-MS Homo sapiens
64 CUL4B 8450
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
65 RPL4 6124
Affinity Capture-MS Homo sapiens
66 Ddb1 13194
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
67 TET2  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
68 PLD1 5337
Affinity Capture-MS Homo sapiens
69 DTNBP1  
Affinity Capture-MS Homo sapiens
70 Kif18a  
Affinity Capture-MS Mus musculus
71 MCM10 55388
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
72 RPL18A 6142
Affinity Capture-MS Homo sapiens
73 UBR5 51366
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
74 SP110  
Affinity Capture-MS Homo sapiens
75 YWHAE 7531
Affinity Capture-MS Homo sapiens
76 MYH9 4627
Co-fractionation Homo sapiens
77 RAG1  
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
78 NTRK1 4914
Affinity Capture-MS Homo sapiens
79 RORA  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
80 XAGE1B  
Affinity Capture-MS Homo sapiens
81 NCL 4691
Co-fractionation Homo sapiens
82 TACSTD2 4070
Affinity Capture-MS Homo sapiens
83 HIST1H1C 3006
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
84 RPL27A 6157
Affinity Capture-MS Homo sapiens
85 MTMR1 8776
Affinity Capture-MS Homo sapiens
86 COPS3 8533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
87 FGFRL1 53834
Affinity Capture-MS Homo sapiens
88 ZFC3H1 196441
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 COPS7B 64708
Affinity Capture-MS Homo sapiens
90 Ksr1  
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
91 SP100 6672
Affinity Capture-MS Homo sapiens
92 WDR46  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 HPSE2  
Affinity Capture-MS Homo sapiens
94 ZNF747  
Affinity Capture-MS Homo sapiens
95 HSPA5 3309
Co-fractionation Homo sapiens
96 RAG2  
Co-purification Homo sapiens
97 COPS2 9318
Affinity Capture-MS Homo sapiens
98 HIST1H3A 8350
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
99 ABT1 29777
Affinity Capture-MS Homo sapiens
100 AURKAIP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
101 MRPL35  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
102 SIRT7  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
103 UBXN7 26043
Affinity Capture-MS Homo sapiens
104 RPL35 11224
Affinity Capture-MS Homo sapiens
105 CLTA 1211
Affinity Capture-MS Homo sapiens
106 Dda1  
Affinity Capture-MS Mus musculus
107 COPS8 10920
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
108 HSP90AB1 3326
Co-fractionation Homo sapiens
109 TET1  
Affinity Capture-Western Homo sapiens
110 UNG  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
111 RPL3 6122
Affinity Capture-MS Homo sapiens
112 TFPT  
Affinity Capture-MS Homo sapiens
113 RPL7 6129
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
114 ZNF223  
Affinity Capture-MS Homo sapiens
115 HIST3H3 8290
Biochemical Activity Homo sapiens
116 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
117 AP2M1 1173
Affinity Capture-MS Homo sapiens
118 CEP250 11190
Affinity Capture-MS Homo sapiens
119 DCUN1D1 54165
Affinity Capture-MS Homo sapiens
120 MYO6 4646
Affinity Capture-MS Homo sapiens
121 PPP2R1A 5518
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
122 Tet2  
Affinity Capture-Western Mus musculus
Biochemical Activity Mus musculus
123 Cep78  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
124 HSPA4 3308
Affinity Capture-MS Homo sapiens
125 HIST1H1A 3024
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
126 DDB1 1642
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
127 Nf2  
Co-crystal Structure Mus musculus
Reconstituted Complex Mus musculus
128 RPL18 6141
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
129 TRIM28 10155
Affinity Capture-MS Homo sapiens
130 PYGO2  
Reconstituted Complex Homo sapiens
131 Tet1  
Affinity Capture-Western Mus musculus
132 Cul4a  
Affinity Capture-MS Mus musculus
133 FTSJ3 117246
Affinity Capture-MS Homo sapiens
134 PYHIN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
135 ZNF263  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
136 SOX2  
Affinity Capture-MS Homo sapiens
137 RPL13 6137
Affinity Capture-MS Homo sapiens
138 DVL2 1856
Affinity Capture-MS Homo sapiens
139 Esrrb  
Affinity Capture-MS Mus musculus
140 LLGL2 3993
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
141 ZNF707  
Affinity Capture-MS Homo sapiens
142 H3F3A 3020
Reconstituted Complex Homo sapiens
143 TOPBP1 11073
Affinity Capture-MS Homo sapiens
144 TP53 7157
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
145 RPL6 6128
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
146 RFWD2  
Affinity Capture-MS Homo sapiens
147 CUL5 8065
Affinity Capture-MS Homo sapiens
148 ZNF276 92822
Affinity Capture-MS Homo sapiens
149 ZNF816  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
150 MAP10  
Affinity Capture-MS Homo sapiens
151 HDAC1 3065
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
152 KATNA1 11104
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
153 EPB41L2 2037
Co-fractionation Homo sapiens
154 UBE2D2 7322
Reconstituted Complex Homo sapiens
155 HDAC8 55869
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
156 ESR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
157 FAM120AOS  
Affinity Capture-MS Homo sapiens
158 MAP1LC3A 84557
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
159 RPS24 6229
Affinity Capture-MS Homo sapiens
160 MOV10 4343
Affinity Capture-RNA Homo sapiens
161 TET3  
Affinity Capture-Western Homo sapiens
162 RPL7A 6130
Affinity Capture-MS Homo sapiens
163 RPL23AP32 56969
Affinity Capture-MS Homo sapiens
164 RDX 5962
Affinity Capture-MS Homo sapiens
165 RPS8 6202
Co-fractionation Homo sapiens
166 CEP78 84131
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
167 BRD1 23774
Affinity Capture-MS Homo sapiens
168 TXNIP 10628
Affinity Capture-MS Homo sapiens
169 RPL23A 6147
Affinity Capture-MS Homo sapiens
170 KRAS 3845
Negative Genetic Homo sapiens
171 RPL36AL 6166
Affinity Capture-MS Homo sapiens
172 COPS4 51138
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
173 NOL9 79707
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
174 TIMM13 26517
Affinity Capture-MS Homo sapiens
175 CUL4A 8451
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
176 PLCD3 113026
Affinity Capture-MS Homo sapiens
177 ESCO2  
Affinity Capture-Western Homo sapiens
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