Gene description for CFAP20
Gene name cilia and flagella associated protein 20
Gene symbol CFAP20
Other names/aliases C16orf80
EVORF
GTL3
fSAP23
Species Homo sapiens
 Database cross references - CFAP20
ExoCarta ExoCarta_29105
Vesiclepedia VP_29105
Entrez Gene 29105
HGNC 29523
UniProt Q9Y6A4  
 CFAP20 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Urine 19056867    
 Gene ontology annotations for CFAP20
Molecular Function
    RNA binding GO:0003723 HDA
    protein binding GO:0005515 IPI
Biological Process
    protein polyglutamylation GO:0018095 IMP
    flagellated sperm motility GO:0030317 ISS
    cilium assembly GO:0060271 IBA
    cilium assembly GO:0060271 IMP
    regulation of cilium beat frequency involved in ciliary motility GO:0060296 IBA
    regulation of cilium beat frequency involved in ciliary motility GO:0060296 IDA
    positive regulation of cell motility GO:2000147 IBA
    positive regulation of cell motility GO:2000147 IDA
    positive regulation of feeding behavior GO:2000253 IDA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    centriole GO:0005814 IDA
    axonemal microtubule GO:0005879 IDA
    axonemal microtubule GO:0005879 ISS
    cilium GO:0005929 IDA
    motile cilium GO:0031514 IBA
    ciliary basal body GO:0036064 IBA
    ciliary basal body GO:0036064 IDA
    sperm flagellum GO:0036126 ISS
    extracellular exosome GO:0070062 HDA
    axonemal B tubule inner sheath GO:0160112 IEA
 Experiment description of studies that identified CFAP20 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for CFAP20
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kif19a  
Affinity Capture-MS Mus musculus
2 RIT1 6016
Negative Genetic Homo sapiens
3 LUC7L2 51631
Affinity Capture-MS Homo sapiens
4 ATG16L1 55054
Affinity Capture-MS Homo sapiens
5 MECP2 4204
Affinity Capture-MS Homo sapiens
6 RABEPK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 MRPL4 51073
Affinity Capture-MS Homo sapiens
8 YAF2  
Affinity Capture-MS Homo sapiens
9 UBE2H 7328
Affinity Capture-MS Homo sapiens
10 ZRANB2 9406
Affinity Capture-MS Homo sapiens
11 KIF14 9928
Affinity Capture-MS Homo sapiens
12 XPO1 7514
Affinity Capture-MS Homo sapiens
13 RBM25 58517
Affinity Capture-MS Homo sapiens
14 SART3 9733
Affinity Capture-MS Homo sapiens
15 ECT2 1894
Affinity Capture-MS Homo sapiens
16 PNN 5411
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 FOXJ1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 CHMP4C 92421
Affinity Capture-MS Homo sapiens
19 MRPS35 60488
Affinity Capture-MS Homo sapiens
20 VPRBP 9730
Affinity Capture-MS Homo sapiens
21 MRPS31  
Affinity Capture-MS Homo sapiens
22 PGAM4 441531
Affinity Capture-MS Homo sapiens
23 RAD18  
Affinity Capture-MS Homo sapiens
24 ARL2BP 23568
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 PDHB 5162
Affinity Capture-MS Homo sapiens
26 TARBP2  
Affinity Capture-MS Homo sapiens
27 PRPF40A 55660
Affinity Capture-MS Homo sapiens
28 HNRNPUL1 11100
Affinity Capture-MS Homo sapiens
29 MRPL44  
Affinity Capture-MS Homo sapiens
30 PLD1 5337
Affinity Capture-MS Homo sapiens
31 ACIN1 22985
Affinity Capture-MS Homo sapiens
32 MRPS28  
Affinity Capture-MS Homo sapiens
33 UFL1 23376
Affinity Capture-MS Homo sapiens
34 SFSWAP  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 KPNA4 3840
Affinity Capture-MS Homo sapiens
36 TBC1D32 221322
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 Pqbp1  
Affinity Capture-MS Mus musculus
38 C12orf49  
Positive Genetic Homo sapiens
39 DICER1  
Affinity Capture-MS Homo sapiens
40 DDA1  
Affinity Capture-MS Homo sapiens
41 TFCP2L1  
Two-hybrid Homo sapiens
42 RFX3 5991
Affinity Capture-MS Homo sapiens
43 CLASRP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 HSF1  
Affinity Capture-MS Homo sapiens
45 PRPF38A 84950
Affinity Capture-MS Homo sapiens
46 RNPS1 10921
Affinity Capture-MS Homo sapiens
47 CIT 11113
Affinity Capture-MS Homo sapiens
48 PLK1 5347
Affinity Capture-MS Homo sapiens
49 CDK20  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 ARRDC3 57561
Affinity Capture-MS Homo sapiens
51 KRAS 3845
Synthetic Lethality Homo sapiens
Negative Genetic Homo sapiens
52 SRRM2 23524
Affinity Capture-MS Homo sapiens
53 BMI1  
Affinity Capture-MS Homo sapiens
54 YWHAG 7532
Affinity Capture-MS Homo sapiens
55 FAM149B1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 C11orf84  
Affinity Capture-MS Homo sapiens
57 NANOG  
Affinity Capture-MS Homo sapiens
58 SF3A2 8175
Affinity Capture-MS Homo sapiens
59 RPA3 6119
Proximity Label-MS Homo sapiens
60 RSRC1  
Affinity Capture-MS Homo sapiens
61 Txn1 22166
Two-hybrid Mus musculus
62 SNRPD3 6634
Affinity Capture-MS Homo sapiens
63 U2AF1 7307
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CFAP20 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here