Gene description for KPNA4
Gene name karyopherin alpha 4 (importin alpha 3)
Gene symbol KPNA4
Other names/aliases IPOA3
QIP1
SRP3
Species Homo sapiens
 Database cross references - KPNA4
ExoCarta ExoCarta_3840
Entrez Gene 3840
HGNC 6397
MIM 602970
UniProt O00629  
 KPNA4 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for KPNA4
Molecular Function
    protein binding GO:0005515 IPI
    protein transporter activity GO:0008565 IEA
Biological Process
    cytokine-mediated signaling pathway GO:0019221 TAS
    NLS-bearing protein import into nucleus GO:0006607 TAS
    response to hydrogen peroxide GO:0042542 IEA
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 TAS
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified KPNA4 in exosomes
1
Experiment ID 226
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✔
CD9|FLOT1
EV Membrane markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
✘ EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for KPNA4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ARRB2  
Affinity Capture-MS Homo sapiens
2 CSE1L 1434
Reconstituted Complex Homo sapiens
3 RECQL 5965
Invitro Homo sapiens
Two-hybrid Homo sapiens
4 KPNB1 3837
Reconstituted Complex Homo sapiens
5 TGM2 7052
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which KPNA4 is involved
PathwayEvidenceSource
ISG15 antiviral mechanism TAS Reactome





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