Gene description for RAI1
Gene name retinoic acid induced 1
Gene symbol RAI1
Other names/aliases SMCR
SMS
Species Homo sapiens
 Database cross references - RAI1
ExoCarta ExoCarta_10743
Vesiclepedia VP_10743
Entrez Gene 10743
HGNC 9834
MIM 607642
UniProt Q7Z5J4  
 RAI1 identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for RAI1
Molecular Function
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    skeletal system development GO:0001501 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    circadian regulation of gene expression GO:0032922 IBA
    circadian regulation of gene expression GO:0032922 IMP
    negative regulation of multicellular organism growth GO:0040015 IEA
    positive regulation of DNA-templated transcription GO:0045893 IDA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IEA
 Experiment description of studies that identified RAI1 in sEVs
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for RAI1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD3EAP  
Proximity Label-MS Homo sapiens
2 GATA1  
Two-hybrid Homo sapiens
3 GOLGA2 2801
Proximity Label-MS Homo sapiens
4 Cbx1  
Affinity Capture-MS Mus musculus
5 RPS6KA5 9252
Two-hybrid Homo sapiens
6 TESC 54997
Two-hybrid Homo sapiens
7 NUMA1 4926
Affinity Capture-MS Homo sapiens
8 MYCN  
Affinity Capture-MS Homo sapiens
9 ASF1A 25842
Proximity Label-MS Homo sapiens
10 HDAC2 3066
Affinity Capture-MS Homo sapiens
11 PARP1 142
Proximity Label-MS Homo sapiens
12 ARF3 377
Affinity Capture-MS Homo sapiens
13 GSK3B 2932
Two-hybrid Homo sapiens
14 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
15 MAPK8IP2  
Two-hybrid Homo sapiens
16 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
17 TPT1 7178
Cross-Linking-MS (XL-MS) Homo sapiens
18 NAGK 55577
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 SIRT7  
Affinity Capture-MS Homo sapiens
20 CSNK1A1 1452
Affinity Capture-MS Homo sapiens
21 PIN1 5300
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
22 H2AFZ 3015
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 TTR 7276
Two-hybrid Homo sapiens
24 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
25 S100A2 6273
Affinity Capture-MS Homo sapiens
26 HMGN5 79366
Affinity Capture-MS Homo sapiens
27 LMNA 4000
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
28 CASP2  
Two-hybrid Homo sapiens
29 NR3C1 2908
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
30 PDAP1 11333
Affinity Capture-MS Homo sapiens
31 RPL3 6122
Affinity Capture-MS Homo sapiens
32 GLI1  
Two-hybrid Homo sapiens
33 CDKN1A  
Two-hybrid Homo sapiens
34 CHD4 1108
Affinity Capture-MS Homo sapiens
35 TRIM68 55128
Affinity Capture-MS Homo sapiens
36 NUP35 129401
Proximity Label-MS Homo sapiens
37 VRK1 7443
Affinity Capture-MS Homo sapiens
38 RBBP4 5928
Affinity Capture-MS Homo sapiens
39 MAPK1 5594
Affinity Capture-MS Homo sapiens
40 CENPA  
Proximity Label-MS Homo sapiens
41 Mta1  
Affinity Capture-MS Mus musculus
42 APEX1 328
Proximity Label-MS Homo sapiens
43 ZNF496  
Two-hybrid Homo sapiens
44 KPNA4 3840
Affinity Capture-MS Homo sapiens
45 CAPZB 832
Affinity Capture-MS Homo sapiens
46 NUP50 10762
Proximity Label-MS Homo sapiens
47 DDIT3 1649
Two-hybrid Homo sapiens
48 GADD45A  
Two-hybrid Homo sapiens
49 DTX2 113878
Proximity Label-MS Homo sapiens
50 HECW2  
Affinity Capture-MS Homo sapiens
51 SSRP1 6749
Affinity Capture-MS Homo sapiens
52 HDAC1 3065
Affinity Capture-MS Homo sapiens
53 TCF20  
Affinity Capture-MS Homo sapiens
54 S100P 6286
Affinity Capture-MS Homo sapiens
55 TOP1 7150
Affinity Capture-MS Homo sapiens
56 CDK2AP1  
Affinity Capture-MS Homo sapiens
57 H1F0 3005
Affinity Capture-MS Homo sapiens
58 RPL23A 6147
Cross-Linking-MS (XL-MS) Homo sapiens
59 DHX40  
Proximity Label-MS Homo sapiens
60 E2F4  
Affinity Capture-MS Homo sapiens
61 AR 367
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
62 MAML3  
Two-hybrid Homo sapiens
63 LZTR1 8216
Two-hybrid Homo sapiens
64 MAD2L2 10459
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 NUCKS1 64710
Affinity Capture-MS Homo sapiens
66 HMGA1 3159
Affinity Capture-MS Homo sapiens
67 HMG20A  
Affinity Capture-MS Homo sapiens
68 BRD3 8019
Affinity Capture-MS Homo sapiens
69 CEBPA  
Proximity Label-MS Homo sapiens
70 PYHIN1  
Affinity Capture-MS Homo sapiens
71 DAXX  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which RAI1 is involved
PathwayEvidenceSource
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Circadian Clock IEA Reactome
Heme signaling IEA Reactome





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