Gene description for DTX2
Gene name deltex 2, E3 ubiquitin ligase
Gene symbol DTX2
Other names/aliases RNF58
Species Homo sapiens
 Database cross references - DTX2
ExoCarta ExoCarta_113878
Vesiclepedia VP_113878
Entrez Gene 113878
HGNC 15973
MIM 613141
UniProt Q86UW9  
 DTX2 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 34887515    
 Gene ontology annotations for DTX2
Molecular Function
    ligase activity GO:0016874 IEA
    zinc ion binding GO:0008270 IEA
    protein binding GO:0005515 IPI
Biological Process
    Notch signaling pathway GO:0007219 IEA
    protein ubiquitination GO:0016567 IEA
Subcellular Localization
    cytoplasm GO:0005737 IEA
    nucleoplasm GO:0005654 IDA
    nuclear membrane GO:0031965 IDA
 Experiment description of studies that identified DTX2 in exosomes
1
Experiment ID 1203
MISEV standards
✔
EM
EV Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ.
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
 Protein-protein interactions for DTX2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NCALD 83988
Two-hybrid Homo sapiens
2 HPCAL1 3241
Two-hybrid Homo sapiens
3 RFXDC1  
Two-hybrid Homo sapiens
4 NCS1 23413
Two-hybrid Homo sapiens
5 SFN 2810
Affinity Capture-MS Homo sapiens
6 TRIP13 9319
Two-hybrid Homo sapiens
7 PLSCR1 5359
Two-hybrid Homo sapiens
8 RBPMS 11030
Two-hybrid Homo sapiens
9 HPCAL4  
Two-hybrid Homo sapiens
10 VSNL1 7447
Two-hybrid Homo sapiens
11 UBE2D4  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
12 PEPP-2  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which DTX2 is involved
PathwayEvidenceSource
Activated NOTCH1 Transmits Signal to the Nucleus TAS Reactome





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