Gene description for SHB
Gene name Src homology 2 domain containing adaptor protein B
Gene symbol SHB
Other names/aliases bA3J10.2
Species Homo sapiens
 Database cross references - SHB
ExoCarta ExoCarta_6461
Vesiclepedia VP_6461
Entrez Gene 6461
HGNC 10838
MIM 600314
UniProt Q15464  
 SHB identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 34887515    
 Gene ontology annotations for SHB
Molecular Function
    SH3/SH2 adaptor activity GO:0005070 TAS
    protein binding GO:0005515 IPI
Biological Process
    positive regulation of signal transduction GO:0009967 TAS
    cell differentiation GO:0030154 IEA
    angiogenesis GO:0001525 IEA
    signal transduction GO:0007165 TAS
    vascular endothelial growth factor receptor signaling pathway GO:0048010 TAS
    apoptotic process GO:0006915 IEA
Subcellular Localization
    plasma membrane GO:0005886 IEA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified SHB in exosomes
1
Experiment ID 1203
MISEV standards
✔
EM
EV Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ.
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
 Protein-protein interactions for SHB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CRK 1398
Invivo Homo sapiens
Invitro Homo sapiens
2 IL2RB  
Reconstituted Complex Homo sapiens
3 SRC 6714
Invivo Homo sapiens
Invitro Homo sapiens
4 JAK3 3718
Reconstituted Complex Homo sapiens
5 PIK3R1 5295
Invitro Homo sapiens
6 LAT 27040
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
7 IL2RG  
Reconstituted Complex Homo sapiens
8 VAV1 7409
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
9 PLCG1 5335
Affinity Capture-Western Homo sapiens
10 KDR  
Affinity Capture-Western Homo sapiens
11 FGFR1 2260
Invivo Homo sapiens
Invitro Homo sapiens
12 EPS8 2059
Invivo Homo sapiens
Invitro Homo sapiens
13 JAK1 3716
Reconstituted Complex Homo sapiens
14 GRB2 2885
Affinity Capture-Western Homo sapiens
15 LCP2 3937
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
16 ZAP70 7535
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which SHB is involved
PathwayEvidenceSource
EPHA-mediated growth cone collapse TAS Reactome
VEGFA-VEGFR2 Pathway TAS Reactome





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