Gene description for FBLIM1
Gene name filamin binding LIM protein 1
Gene symbol FBLIM1
Other names/aliases CAL
FBLP-1
FBLP1
Species Homo sapiens
 Database cross references - FBLIM1
ExoCarta ExoCarta_54751
Vesiclepedia VP_54751
Entrez Gene 54751
HGNC 24686
MIM 607747
UniProt Q8WUP2  
 FBLIM1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for FBLIM1
Molecular Function
    protein binding GO:0005515 IPI
    filamin binding GO:0031005 IBA
    filamin binding GO:0031005 IDA
    metal ion binding GO:0046872 IEA
Biological Process
    regulation of cell shape GO:0008360 IEA
    regulation of integrin activation GO:0033623 IMP
    cell-cell adhesion GO:0098609 IBA
    cell-cell adhesion GO:0098609 IMP
Subcellular Localization
    fibrillar center GO:0001650 IDA
    stress fiber GO:0001725 IBA
    stress fiber GO:0001725 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    focal adhesion GO:0005925 HDA
    focal adhesion GO:0005925 IBA
    focal adhesion GO:0005925 IDA
    cell junction GO:0030054 IDA
    cell periphery GO:0071944 IDA
 Experiment description of studies that identified FBLIM1 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for FBLIM1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FAN1  
Affinity Capture-MS Homo sapiens
2 SHKBP1  
Affinity Capture-MS Homo sapiens
3 FAM101B  
Affinity Capture-MS Homo sapiens
4 TRIM15  
Affinity Capture-Western Homo sapiens
PCA Homo sapiens
5 TRIP6 7205
Affinity Capture-MS Homo sapiens
6 HIST1H3F 8968
Affinity Capture-MS Homo sapiens
7 ADAT3 113179
Two-hybrid Homo sapiens
8 HIST1H3A 8350
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 NIF3L1 60491
Affinity Capture-MS Homo sapiens
10 NKX2-5  
Affinity Capture-MS Homo sapiens
11 CUTA 51596
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 H3F3B 3021
Affinity Capture-MS Homo sapiens
13 XPO1 7514
Affinity Capture-MS Homo sapiens
14 PKP2 5318
Affinity Capture-MS Homo sapiens
15 ALMS1  
Affinity Capture-MS Homo sapiens
16 C19orf54  
Two-hybrid Homo sapiens
17 FERMT2 10979
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
18 DCLK2 166614
Affinity Capture-MS Homo sapiens
19 CUL3 8452
Affinity Capture-MS Homo sapiens
20 HIST1H3E 8353
Affinity Capture-MS Homo sapiens
21 DIXDC1  
Affinity Capture-MS Homo sapiens
22 HIST1H3C 8352
Affinity Capture-MS Homo sapiens
23 EVL 51466
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 VASP 7408
Affinity Capture-MS Homo sapiens
25 ILVBL 10994
Affinity Capture-MS Homo sapiens
26 CBL 867
Affinity Capture-MS Homo sapiens
27 ENAH 55740
Affinity Capture-MS Homo sapiens
28 HIST1H3B 8358
Affinity Capture-MS Homo sapiens
29 HIST1H3J 8356
Affinity Capture-MS Homo sapiens
30 C11orf30  
Affinity Capture-MS Homo sapiens
31 NDE1 54820
Affinity Capture-MS Homo sapiens
32 PAPSS1 9061
Affinity Capture-MS Homo sapiens
33 FLNA 2316
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
34 ANXA5 308
Co-fractionation Homo sapiens
35 FLNC 2318
Affinity Capture-MS Homo sapiens
36 FLNB 2317
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
37 SHB 6461
Affinity Capture-MS Homo sapiens
38 HIST1H3I 8354
Affinity Capture-MS Homo sapiens
39 TMCC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 KCTD3  
Affinity Capture-MS Homo sapiens
41 SHISA6  
Two-hybrid Homo sapiens
42 LMO3  
Two-hybrid Homo sapiens
43 DCLK1 9201
Affinity Capture-MS Homo sapiens
44 H3F3C 440093
Two-hybrid Homo sapiens
45 KIF18A  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which FBLIM1 is involved
PathwayEvidenceSource
Cell junction organization TAS Reactome
Cell-Cell communication TAS Reactome
Cell-extracellular matrix interactions TAS Reactome





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