Gene description for ENAH
Gene name enabled homolog (Drosophila)
Gene symbol ENAH
Other names/aliases ENA
MENA
NDPP1
Species Homo sapiens
 Database cross references - ENAH
ExoCarta ExoCarta_55740
Vesiclepedia VP_55740
Entrez Gene 55740
HGNC 18271
MIM 609061
UniProt Q8N8S7  
 ENAH identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Melanoma cells 25950383    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for ENAH
Molecular Function
    actin binding GO:0003779 IEA
    protein binding GO:0005515 IPI
    profilin binding GO:0005522 IBA
    SH3 domain binding GO:0017124 IEA
    WW domain binding GO:0050699 IPI
Biological Process
    axon guidance GO:0007411 IBA
    actin polymerization or depolymerization GO:0008154 IBA
    actin polymerization-dependent cell motility GO:0070358 IBA
    postsynaptic cytoskeleton organization GO:0099188 IEA
Subcellular Localization
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    focal adhesion GO:0005925 HDA
    focal adhesion GO:0005925 IBA
    focal adhesion GO:0005925 IDA
    lamellipodium GO:0030027 IEA
    cell junction GO:0030054 IDA
    filopodium GO:0030175 IEA
    postsynapse GO:0098794 IEA
    GABA-ergic synapse GO:0098982 IEA
 Experiment description of studies that identified ENAH in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 260
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ENAH
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 AJUBA  
Affinity Capture-MS Homo sapiens
3 XIRP1  
Two-hybrid Homo sapiens
4 RAC1 5879
FRET Homo sapiens
Reconstituted Complex Homo sapiens
5 PPP6R3 55291
Co-fractionation Homo sapiens
6 ABI3 51225
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
7 OSGEP 55644
Affinity Capture-MS Homo sapiens
8 UBE2H 7328
Affinity Capture-MS Homo sapiens
9 TES 26136
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
10 TP53 7157
Affinity Capture-MS Homo sapiens
11 DNAJB6 10049
Affinity Capture-MS Homo sapiens
12 DYRK1B  
Affinity Capture-MS Homo sapiens
13 BAG2 9532
Affinity Capture-MS Homo sapiens
14 WWOX 51741
Affinity Capture-MS Homo sapiens
15 C9orf78 51759
Affinity Capture-MS Homo sapiens
16 MAPK1 5594
Two-hybrid Homo sapiens
17 PFN1 5216
Reconstituted Complex Homo sapiens
Proximity Label-MS Homo sapiens
18 ZYX 7791
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Protein-peptide Homo sapiens
19 XPO1 7514
Affinity Capture-MS Homo sapiens
20 KLHDC3  
Two-hybrid Homo sapiens
21 ATG13 9776
Affinity Capture-MS Homo sapiens
22 TPTE  
Affinity Capture-MS Homo sapiens
23 ACTC1 70
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
24 PFN2 5217
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 PRMT6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 BCHE 590
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
28 TERF2IP 54386
Two-hybrid Homo sapiens
29 PARK2  
Affinity Capture-MS Homo sapiens
30 COPS4 51138
Co-fractionation Homo sapiens
31 CTDP1 9150
Affinity Capture-MS Homo sapiens
32 DNMBP 23268
Reconstituted Complex Homo sapiens
33 DLGAP4 22839
Affinity Capture-MS Homo sapiens
34 EVL 51466
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
35 RAPH1 65059
Affinity Capture-Western Homo sapiens
36 NUDT3 11165
Co-fractionation Homo sapiens
37 XPO6 23214
Affinity Capture-MS Homo sapiens
38 PDZD8 118987
Affinity Capture-MS Homo sapiens
39 LGALS3BP 3959
Affinity Capture-MS Homo sapiens
40 Apbb1  
Reconstituted Complex Rattus norvegicus
41 CERS2 29956
Affinity Capture-MS Homo sapiens
42 CAPZB 832
Affinity Capture-MS Homo sapiens
43 RPA3 6119
Proximity Label-MS Homo sapiens
44 ABI2 10152
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
45 NCKAP1 10787
Affinity Capture-MS Homo sapiens
46 TRIM28 10155
Co-fractionation Homo sapiens
47 SEPT7 989
Co-fractionation Homo sapiens
48 HECW2  
Affinity Capture-MS Homo sapiens
49 BBS1 582
Affinity Capture-MS Homo sapiens
50 DYRK1A 1859
Affinity Capture-MS Homo sapiens
51 ARRB1 408
Co-fractionation Homo sapiens
52 NTRK1 4914
Affinity Capture-MS Homo sapiens
53 DCAF7 10238
Affinity Capture-MS Homo sapiens
54 GPHN 10243
Affinity Capture-Western Homo sapiens
55 TMOD3 29766
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 APBB1  
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Protein-peptide Homo sapiens
57 LPIN3 64900
Affinity Capture-MS Homo sapiens
58 PPM1K  
Affinity Capture-MS Homo sapiens
59 ACTB 60
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 BICD2 23299
Proximity Label-MS Homo sapiens
61 FBLIM1 54751
Affinity Capture-MS Homo sapiens
62 PRPF4 9128
Affinity Capture-MS Homo sapiens
63 SNW1 22938
Affinity Capture-MS Homo sapiens
64 PVRL4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 PARL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 SMURF2 64750
Affinity Capture-MS Homo sapiens
67 ABI1 10006
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
68 ATXN3 4287
Affinity Capture-MS Homo sapiens
69 ATOH1  
Affinity Capture-MS Homo sapiens
70 EMC9  
Affinity Capture-MS Homo sapiens
71 YAP1 10413
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
72 BAIAP2 10458
Reconstituted Complex Homo sapiens
73 ACTG1 71
Affinity Capture-MS Homo sapiens
74 FANCD2  
Cross-Linking-MS (XL-MS) Homo sapiens
75 VASP 7408
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 CDC5L 988
Affinity Capture-MS Homo sapiens
77 EP300 2033
Affinity Capture-MS Homo sapiens
78 TACC3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 KIAA1522 57648
Affinity Capture-MS Homo sapiens
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