Gene description for ENAH
Gene name enabled homolog (Drosophila)
Gene symbol ENAH
Other names/aliases ENA
MENA
NDPP1
Species Homo sapiens
 Database cross references - ENAH
ExoCarta ExoCarta_55740
Entrez Gene 55740
HGNC 18271
MIM 609061
UniProt Q8N8S7  
 ENAH identified in exosomes derived from the following tissue/cell type
Melanoma cells 25950383    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for ENAH
Molecular Function
    actin binding GO:0003779 IEA
    SH3 domain binding GO:0017124 IEA
    WW domain binding GO:0050699 IPI
    protein binding GO:0005515 IPI
Biological Process
    T cell receptor signaling pathway GO:0050852 TAS
    intracellular transport GO:0046907 IEA
    actin polymerization or depolymerization GO:0008154 IEA
    axon guidance GO:0007411 TAS
    neural tube closure GO:0001843 IEA
Subcellular Localization
    filopodium GO:0030175 IEA
    synapse GO:0045202 IEA
    focal adhesion GO:0005925 IDA
    lamellipodium GO:0030027 IEA
    stress fiber GO:0001725 IEA
    cytosol GO:0005829 TAS
    cell junction GO:0030054 IDA
    cytoplasm GO:0005737 IDA
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified ENAH in exosomes
1
Experiment ID 260
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ENAH
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PFN1 5216
Invivo Homo sapiens
Invitro Homo sapiens
2 ZYX 7791
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
3 XPO6 23214
Affinity Capture-MS Homo sapiens
4 TES 26136
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
5 ABI1 10006
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
6 BAIAP2 10458
Reconstituted Complex Homo sapiens
7 DNMBP  
Reconstituted Complex Homo sapiens
8 RAPH1 65059
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which ENAH is involved
PathwayEvidenceSource
Generation of second messenger molecules TAS Reactome
Signaling by Robo receptor TAS Reactome





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