Gene description for TRIM28
Gene name
tripartite motif containing 28
Gene symbol
TRIM28
Other names/aliases
KAP1 PPP1R157 RNF96 TF1B TIF1B
Species
Homo sapiens
Database cross references - TRIM28
ExoCarta
ExoCarta_10155
Entrez Gene
10155
HGNC
16384
MIM
601742
UniProt
Q13263
TRIM28 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells
25944692
Ovarian cancer cells
23333927
Ovarian cancer cells
23333927
Thymus
23844026
Gene ontology annotations for TRIM28
Experiment description of studies that identified TRIM28 in exosomes
1
Experiment ID
224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25944692
Organism
Homo sapiens
Experiment description
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors
Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name
Oncotarget
Publication year
2015
Sample
Neuroblastoma cells
Sample name
SH-SY5Y
Isolation/purification methods
Differential centrifugation Ultracentrifugation OptiPrep density gradient
Flotation density
1.10 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
2
Experiment ID
211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
IGROV1
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
3
Experiment ID
212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
OVCAR-3
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
4
Experiment ID
217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23844026
Organism
Homo sapiens
Experiment description
Characterization of human thymic exosomes.
Authors
Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name
PLoS One
Publication year
2013
Sample
Thymus
Sample name
Normal-Thymus
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
Protein-protein interactions for TRIM28
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
CEBPB
Invivo
Homo sapiens
Invivo
Homo sapiens
Invitro
Homo sapiens
Invitro
Homo sapiens
2
TFE3
Affinity Capture-MS
Homo sapiens
3
CBX3
11335
Invivo
Homo sapiens
Invitro
Homo sapiens
Affinity Capture-Western
Homo sapiens
4
TNFRSF10D
8793
Affinity Capture-MS
Homo sapiens
5
ZNF197
Reconstituted Complex
Homo sapiens
Affinity Capture-Western
Homo sapiens
6
CBX1
10951
Invivo
Homo sapiens
Invitro
Homo sapiens
Two-hybrid
Homo sapiens
Affinity Capture-Western
Homo sapiens
7
NCOR1
Co-purification
Homo sapiens
8
TRIM28
10155
FRET
Homo sapiens
Affinity Capture-Western
Homo sapiens
Co-purification
Homo sapiens
FRET
Homo sapiens
Affinity Capture-Western
Homo sapiens
Co-purification
Homo sapiens
9
VIM
7431
Two-hybrid
Homo sapiens
10
NR3C1
2908
Invivo
Homo sapiens
Invitro
Homo sapiens
11
SPG7
Two-hybrid
Homo sapiens
12
TIF1
FRET
Homo sapiens
13
VHL
Reconstituted Complex
Homo sapiens
14
ZNF74
FRET
Homo sapiens
15
TRIM6
Co-purification
Homo sapiens
16
CHD3
Two-hybrid
Homo sapiens
17
CBX5
23468
Invivo
Homo sapiens
Invitro
Homo sapiens
Two-hybrid
Homo sapiens
Affinity Capture-Western
Homo sapiens
Two-hybrid
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
18
SETDB1
Affinity Capture-MS
Homo sapiens
Two-hybrid
Homo sapiens
19
HLA-B
Affinity Capture-MS
Homo sapiens
20
ZNF10
Two-hybrid
Homo sapiens
Reconstituted Complex
Homo sapiens
Co-purification
Homo sapiens
21
TRIM23
373
Co-purification
Homo sapiens
View the network
image/svg+xml
Pathways in which TRIM28 is involved