Gene description for CBX5
Gene name chromobox homolog 5
Gene symbol CBX5
Other names/aliases HEL25
HP1
HP1A
Species Homo sapiens
 Database cross references - CBX5
ExoCarta ExoCarta_23468
Entrez Gene 23468
HGNC 1555
MIM 604478
UniProt P45973  
 CBX5 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for CBX5
Molecular Function
    protein binding, bridging GO:0030674 ISS
    protein homodimerization activity GO:0042803 IEA
    methylated histone binding GO:0035064 IDA
    protein binding GO:0005515 IPI
    chromatin binding GO:0003682 IEA
    histone deacetylase binding GO:0042826 IEA
    repressing transcription factor binding GO:0070491 ISS
Biological Process
    blood coagulation GO:0007596 TAS
    negative regulation of transcription, DNA-templated GO:0045892 IMP
    viral process GO:0016032 IEA
Subcellular Localization
    chromocenter GO:0010369 IEA
    nucleolus GO:0005730 IDA
    nucleus GO:0005634 IDA
    transcriptional repressor complex GO:0017053 ISS
    PML body GO:0016605 IMP
    nucleoplasm GO:0005654 TAS
    nuclear pericentric heterochromatin GO:0031618 NAS
    nuclear envelope GO:0005635 TAS
    histone methyltransferase complex GO:0035097 ISS
    nuclear heterochromatin GO:0005720 TAS
    kinetochore GO:0000776 IEA
    histone deacetylase complex GO:0000118 ISS
 Experiment description of studies that identified CBX5 in exosomes
1
Experiment ID 226
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✔
CD9|FLOT1
EV Membrane markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
✘ EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CBX5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DSN1 79980
Affinity Capture-Western Homo sapiens
2 RPSA 3921
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
3 LBR 3930
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
4 HIST3H2A 92815
Reconstituted Complex Homo sapiens
5 C1orf48  
Affinity Capture-Western Homo sapiens
6 HDAC4  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
7 CBX1 10951
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
8 SP100  
Affinity Capture-MS Homo sapiens
9 MIS12  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
10 RB1 5925
Affinity Capture-Western Homo sapiens
11 HDAC9  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Phenotypic Enhancement Homo sapiens
12 HIST3H2BB 128312
Reconstituted Complex Homo sapiens
13 ARL5  
Invitro Homo sapiens
Two-hybrid Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
14 HDAC1 3065
Affinity Capture-Western Homo sapiens
15 HIST4H4 121504
Reconstituted Complex Homo sapiens
16 INCENP  
Invitro Homo sapiens
Two-hybrid Homo sapiens
17 DNMT3A 1788
Affinity Capture-Western Homo sapiens
18 TBP  
Affinity Capture-Western Homo sapiens
19 CBX3 11335
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
20 SMARCA4 6597
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
21 DNMT3B  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
22 TRIM28 10155
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
23 MGC3121  
Two-hybrid Homo sapiens
24 CHAF1A  
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
25 CBX5 23468
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
26 HIST2H3A 333932
Reconstituted Complex Homo sapiens
27 XRCC6 2547
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
28 SUV39H1  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
29 MBD1  
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
30 CHAF1B  
Two-hybrid Homo sapiens
31 DNMT1 1786
Affinity Capture-Western Homo sapiens
32 TIF1  
Invitro Homo sapiens
Two-hybrid Homo sapiens
33 HDAC5 10014
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
34 TAF4 6874
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
35 TCEB3B  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which CBX5 is involved
PathwayEvidenceSource
Factors involved in megakaryocyte development and platelet production TAS Reactome





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