Gene description for PPP2R5E
Gene name protein phosphatase 2, regulatory subunit B', epsilon isoform
Gene symbol PPP2R5E
Other names/aliases -
Species Homo sapiens
 Database cross references - PPP2R5E
ExoCarta ExoCarta_5529
Vesiclepedia VP_5529
Entrez Gene 5529
HGNC 9313
MIM 601647
UniProt Q16537  
 PPP2R5E identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Thymus 23844026    
 Gene ontology annotations for PPP2R5E
Molecular Function
    protein binding GO:0005515 IPI
    protein phosphatase regulator activity GO:0019888 TAS
    protein phosphatase activator activity GO:0072542 IBA
Biological Process
    signal transduction GO:0007165 IEA
Subcellular Localization
    protein phosphatase type 2A complex GO:0000159 IBA
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 TAS
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
 Experiment description of studies that identified PPP2R5E in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
7
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PPP2R5E
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sgol2  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
2 VTI1B 10490
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 RORC 6097
Two-hybrid Homo sapiens
4 TPD52L2 7165
Co-fractionation Homo sapiens
5 TMEM206 55248
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 DEC1  
Two-hybrid Homo sapiens
7 C9orf78 51759
Affinity Capture-MS Homo sapiens
8 Soga1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
9 CHEK2  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
10 TERF2IP 54386
Co-fractionation Homo sapiens
11 Nhsl1  
Affinity Capture-MS Mus musculus
12 LRPPRC 10128
Co-fractionation Homo sapiens
13 PPP2R1B 5519
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
14 PPP2R5C 5527
Co-fractionation Homo sapiens
15 KLF15  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 SOGA3  
Affinity Capture-MS Homo sapiens
17 ELP4  
Affinity Capture-MS Homo sapiens
18 KNDC1  
Affinity Capture-MS Homo sapiens
19 CSNK2B 1460
Two-hybrid Homo sapiens
20 SAMHD1 25939
Affinity Capture-MS Homo sapiens
21 NANOG  
Affinity Capture-MS Homo sapiens
22 DNASE2B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 ZDHHC17 23390
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
24 VSIG1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 PPP1CA 5499
Two-hybrid Homo sapiens
26 S100A2 6273
Affinity Capture-MS Homo sapiens
27 BHLHE41  
Two-hybrid Homo sapiens
28 SPX  
Affinity Capture-MS Homo sapiens
29 CRY1  
Two-hybrid Homo sapiens
30 MOS 4342
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 SAE1 10055
Co-fractionation Homo sapiens
32 FAM9C  
Affinity Capture-MS Homo sapiens
33 NOD2 64127
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
34 CERS2 29956
Affinity Capture-MS Homo sapiens
35 GJB7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 ACTN4 81
Co-fractionation Homo sapiens
37 OGT 8473
Reconstituted Complex Homo sapiens
38 CBX5 23468
Affinity Capture-MS Homo sapiens
39 PPP2CA 5515
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
40 YWHAQ 10971
Co-fractionation Homo sapiens
41 CRY2  
Two-hybrid Homo sapiens
42 CSNK1E 1454
Two-hybrid Homo sapiens
43 GRIN2A  
Affinity Capture-MS Homo sapiens
44 Ksr1  
Affinity Capture-MS Mus musculus
45 CCNG2  
Affinity Capture-MS Homo sapiens
46 FAM43A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 KLHL14 57565
Affinity Capture-MS Homo sapiens
48 CDK17 5128
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 Ppp2r1a 51792
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
50 C19orf57  
Affinity Capture-MS Homo sapiens
51 Gtf2e2  
Affinity Capture-MS Mus musculus
52 ZFPL1 7542
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 WNT3A 89780
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 CUL3 8452
Affinity Capture-MS Homo sapiens
55 SGOL1  
Affinity Capture-MS Homo sapiens
56 EGFR 1956
Affinity Capture-MS Homo sapiens
57 DCLRE1C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 RPS27 6232
Affinity Capture-MS Homo sapiens
59 FBXW11  
Two-hybrid Homo sapiens
60 C3orf18  
Affinity Capture-MS Homo sapiens
61 FAM13A 10144
Affinity Capture-MS Homo sapiens
62 RPA3 6119
Proximity Label-MS Homo sapiens
63 SMAD9  
Two-hybrid Homo sapiens
64 ZNF785  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 CCNG1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 PPP2R5A 5525
Affinity Capture-MS Homo sapiens
67 AARS 16
Co-fractionation Homo sapiens
68 PPP2CB 5516
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 APP 351
Reconstituted Complex Homo sapiens
70 FASN 2194
Negative Genetic Homo sapiens
71 PPP2R5E 5529
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
72 CDC20 991
Affinity Capture-MS Homo sapiens
73 TRMT10A 93587
Co-fractionation Homo sapiens
74 CRIP1 1396
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 ZNF276 92822
Affinity Capture-MS Homo sapiens
76 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
77 DCAF11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 PRR14  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 IK 3550
Co-fractionation Homo sapiens
80 API5 8539
Co-fractionation Homo sapiens
81 MARK2 2011
Affinity Capture-MS Homo sapiens
82 C16orf72 29035
Affinity Capture-MS Homo sapiens
83 CLOCK  
Two-hybrid Homo sapiens
84 ZNF517  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 C1orf21  
Affinity Capture-MS Homo sapiens
86 BRD1 23774
Affinity Capture-MS Homo sapiens
87 LPAR4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 DDX56  
Affinity Capture-MS Homo sapiens
90 C1QL4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 AIM1 202
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PPP2R5E is involved
PathwayEvidenceSource
Adaptive Immune System TAS Reactome
Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal TAS Reactome
Amplification of signal from the kinetochores TAS Reactome
APC truncation mutants have impaired AXIN binding TAS Reactome
AXIN missense mutants destabilize the destruction complex TAS Reactome
Beta-catenin phosphorylation cascade TAS Reactome
Cell Cycle TAS Reactome
Cell Cycle Checkpoints TAS Reactome
Cell Cycle, Mitotic TAS Reactome
Costimulation by the CD28 family TAS Reactome
CTLA4 inhibitory signaling TAS Reactome
CTNNB1 S33 mutants aren't phosphorylated TAS Reactome
CTNNB1 S37 mutants aren't phosphorylated TAS Reactome
CTNNB1 S45 mutants aren't phosphorylated TAS Reactome
CTNNB1 T41 mutants aren't phosphorylated TAS Reactome
Degradation of beta-catenin by the destruction complex TAS Reactome
Disassembly of the destruction complex and recruitment of AXIN to the membrane TAS Reactome
Disassembly of the destruction complex and recruitment of AXIN to the membrane IEA Reactome
Disease TAS Reactome
Diseases of signal transduction by growth factor receptors and second messengers TAS Reactome
EML4 and NUDC in mitotic spindle formation TAS Reactome
Hemostasis TAS Reactome
Immune System TAS Reactome
Intracellular signaling by second messengers TAS Reactome
M Phase TAS Reactome
MAPK family signaling cascades TAS Reactome
MAPK1/MAPK3 signaling TAS Reactome
Mitotic Anaphase TAS Reactome
Mitotic Metaphase and Anaphase TAS Reactome
Mitotic Prometaphase TAS Reactome
Mitotic Spindle Checkpoint TAS Reactome
Negative regulation of MAPK pathway TAS Reactome
Negative regulation of the PI3K/AKT network TAS Reactome
PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling TAS Reactome
PIP3 activates AKT signaling TAS Reactome
Platelet homeostasis TAS Reactome
Platelet sensitization by LDL TAS Reactome
RAF activation TAS Reactome
RAF/MAP kinase cascade TAS Reactome
Resolution of Sister Chromatid Cohesion TAS Reactome
RHO GTPase Effectors TAS Reactome
RHO GTPases Activate Formins TAS Reactome
Separation of Sister Chromatids TAS Reactome
Signal Transduction TAS Reactome
Signal Transduction IEA Reactome
Signaling by AMER1 mutants TAS Reactome
Signaling by APC mutants TAS Reactome
Signaling by AXIN mutants TAS Reactome
Signaling by CTNNB1 phospho-site mutants TAS Reactome
Signaling by GSK3beta mutants TAS Reactome
Signaling by Rho GTPases TAS Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 TAS Reactome
Signaling by WNT TAS Reactome
Signaling by WNT IEA Reactome
Signaling by WNT in cancer TAS Reactome
TCF dependent signaling in response to WNT TAS Reactome
TCF dependent signaling in response to WNT IEA Reactome
Truncations of AMER1 destabilize the destruction complex TAS Reactome





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