Gene description for PPP2CB
Gene name protein phosphatase 2, catalytic subunit, beta isozyme
Gene symbol PPP2CB
Other names/aliases PP2Abeta
PP2CB
Species Homo sapiens
 Database cross references - PPP2CB
ExoCarta ExoCarta_5516
Vesiclepedia VP_5516
Entrez Gene 5516
HGNC 9300
MIM 176916
UniProt P62714  
 PPP2CB identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Thymus 23844026    
 Gene ontology annotations for PPP2CB
Molecular Function
    protein C-terminus binding GO:0008022 IEA
    protein serine/threonine phosphatase activity GO:0004722 TAS
    metal ion binding GO:0046872 IEA
    protein binding GO:0005515 IPI
Biological Process
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    negative regulation of Ras protein signal transduction GO:0046580 IEA
    apoptotic mitochondrial changes GO:0008637 IEA
    response to antibiotic GO:0046677 IEA
    response to hydrogen peroxide GO:0042542 IEA
    response to endoplasmic reticulum stress GO:0034976 IEA
    protein dephosphorylation GO:0006470 TAS
    regulation of gene expression GO:0010468 IEA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IEA
Subcellular Localization
    protein phosphatase type 2A complex GO:0000159 TAS
    nucleus GO:0005634 IEA
    spindle pole GO:0000922 IEA
    chromosome, centromeric region GO:0000775 IEA
    cytosol GO:0005829 TAS
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified PPP2CB in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 224
MISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101|CD63|CD81
EV Enriched markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
6
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PPP2CB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 IGBP1 3476
Affinity Capture-MS Homo sapiens
2 STRIP1 85369
Affinity Capture-MS Homo sapiens
3 TLX1  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
4 RELA 5970
Reconstituted Complex Homo sapiens
5 ARL2 402
Co-purification Homo sapiens
6 PPP4C 5531
Affinity Capture-MS Homo sapiens
7 STK24 8428
Affinity Capture-MS Homo sapiens
8 CCT8 10694
Affinity Capture-MS Homo sapiens
9 CCT3 7203
Affinity Capture-MS Homo sapiens
10 TIPRL 261726
Reconstituted Complex Homo sapiens
11 CCT2 10576
Affinity Capture-MS Homo sapiens
12 STRN4 29888
Affinity Capture-MS Homo sapiens
13 TCP1 6950
Affinity Capture-MS Homo sapiens
14 STRN3 29966
Affinity Capture-MS Homo sapiens
15 CCT4 10575
Affinity Capture-MS Homo sapiens
16 MOB4 25843
Affinity Capture-MS Homo sapiens
17 DKFZp547A023  
Affinity Capture-MS Homo sapiens
18 CCT7 10574
Affinity Capture-MS Homo sapiens
19 CCT5 22948
Affinity Capture-MS Homo sapiens
20 CCT6A 908
Affinity Capture-MS Homo sapiens
21 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
22 PPP2R5D 5528
Affinity Capture-MS Homo sapiens
23 STRN 6801
Affinity Capture-MS Homo sapiens
24 PPP2R2A 5520
Affinity Capture-MS Homo sapiens
25 CTTNBP2  
Affinity Capture-MS Homo sapiens
26 FGFR1OP  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PPP2CB is involved
PathwayEvidenceSource
APC truncation mutants have impaired AXIN binding TAS Reactome
AXIN missense mutants destabilize the destruction complex TAS Reactome
Beta-catenin phosphorylation cascade TAS Reactome
CTLA4 inhibitory signaling TAS Reactome
Cyclin A/B1 associated events during G2/M transition TAS Reactome
Cyclin D associated events in G1 TAS Reactome
DARPP-32 events TAS Reactome
Degradation of beta-catenin by the destruction complex TAS Reactome
disassembly of the destruction complex and recruitment of AXIN to the membrane TAS Reactome
ERK/MAPK targets TAS Reactome
ERK/MAPK targets TAS Reactome
ERKs are inactivated TAS Reactome
ERKs are inactivated TAS Reactome
Glycolysis TAS Reactome
Inhibition of replication initiation of damaged DNA by RB1/E2F1 TAS Reactome
Integration of energy metabolism TAS Reactome
MASTL Facilitates Mitotic Progression TAS Reactome
misspliced GSK3beta mutants stabilize beta-catenin TAS Reactome
Mitotic Prometaphase TAS Reactome
Platelet sensitization by LDL TAS Reactome
PP2A-mediated dephosphorylation of key metabolic factors TAS Reactome
Resolution of Sister Chromatid Cohesion TAS Reactome
RHO GTPases Activate Formins TAS Reactome
S33 mutants of beta-catenin aren't phosphorylated TAS Reactome
S37 mutants of beta-catenin aren't phosphorylated TAS Reactome
S45 mutants of beta-catenin aren't phosphorylated TAS Reactome
Separation of Sister Chromatids TAS Reactome
Spry regulation of FGF signaling TAS Reactome
Spry regulation of FGF signaling TAS Reactome
Spry regulation of FGF signaling TAS Reactome
Spry regulation of FGF signaling TAS Reactome
T41 mutants of beta-catenin aren't phosphorylated TAS Reactome
truncations of AMER1 destabilize the destruction complex TAS Reactome





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