Gene description for OTUD1
Gene name OTU deubiquitinase 1
Gene symbol OTUD1
Other names/aliases DUBA7
OTDC1
Species Homo sapiens
 Database cross references - OTUD1
ExoCarta ExoCarta_220213
Vesiclepedia VP_220213
Entrez Gene 220213
HGNC 27346
MIM 612022
UniProt Q5VV17  
 OTUD1 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for OTUD1
Molecular Function
    cysteine-type deubiquitinase activity GO:0004843 IBA
    cysteine-type deubiquitinase activity GO:0004843 IDA
    protein binding GO:0005515 IPI
Biological Process
    proteolysis GO:0006508 IEA
    protein K63-linked deubiquitination GO:0070536 IDA
 Experiment description of studies that identified OTUD1 in exosomes
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for OTUD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RPSA 3921
Affinity Capture-MS Homo sapiens
2 EIF3A 8661
Affinity Capture-MS Homo sapiens
3 MCM7 4176
Affinity Capture-MS Homo sapiens
4 CCT3 7203
Affinity Capture-MS Homo sapiens
5 EEF1G 1937
Affinity Capture-MS Homo sapiens
6 PKM 5315
Affinity Capture-MS Homo sapiens
7 TARDBP 23435
Affinity Capture-MS Homo sapiens
8 FMR1 2332
Affinity Capture-MS Homo sapiens
9 SLC25A1 6576
Affinity Capture-MS Homo sapiens
10 MCM3 4172
Affinity Capture-MS Homo sapiens
11 KPNB1 3837
Affinity Capture-MS Homo sapiens
12 PAICS 10606
Affinity Capture-MS Homo sapiens
13 RPL10 6134
Affinity Capture-MS Homo sapiens
14 RPL15 6138
Affinity Capture-MS Homo sapiens
15 HSP90AB1 3326
Affinity Capture-MS Homo sapiens
16 EIF4A1 1973
Affinity Capture-MS Homo sapiens
17 KPNA2 3838
Affinity Capture-MS Homo sapiens
18 FUBP3 8939
Affinity Capture-MS Homo sapiens
19 RIPK1 8737
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
20 SF3B2 10992
Affinity Capture-MS Homo sapiens
21 LRPPRC 10128
Affinity Capture-MS Homo sapiens
22 PSMC5 5705
Affinity Capture-MS Homo sapiens
23 VARS 7407
Affinity Capture-MS Homo sapiens
24 NUP153 9972
Affinity Capture-MS Homo sapiens
25 CDKN2AIP  
Affinity Capture-MS Homo sapiens
26 ACTG2 72
Affinity Capture-MS Homo sapiens
27 PSMC2 5701
Affinity Capture-MS Homo sapiens
28 KHSRP 8570
Affinity Capture-MS Homo sapiens
29 PRRC2B  
Affinity Capture-MS Homo sapiens
30 ATP5O 539
Affinity Capture-MS Homo sapiens
31 SNRPD3 6634
Affinity Capture-MS Homo sapiens
32 DDX6 1656
Affinity Capture-MS Homo sapiens
33 ZNF598 90850
Affinity Capture-MS Homo sapiens
34 WIZ 58525
Affinity Capture-MS Homo sapiens
35 SEC23A 10484
Affinity Capture-MS Homo sapiens
36 AIMP2 7965
Affinity Capture-MS Homo sapiens
37 RAD23A 5886
Affinity Capture-MS Homo sapiens
38 GTF2I 2969
Affinity Capture-MS Homo sapiens
39 STOML2 30968
Affinity Capture-MS Homo sapiens
40 AURKA 6790
Affinity Capture-Western Homo sapiens
41 RAD23B 5887
Affinity Capture-MS Homo sapiens
42 TCP1 6950
Affinity Capture-MS Homo sapiens
43 BAG2 9532
Affinity Capture-MS Homo sapiens
44 BCL2L11  
Affinity Capture-Western Homo sapiens
45 USP11 8237
Affinity Capture-MS Homo sapiens
46 PEG10 23089
Affinity Capture-MS Homo sapiens
47 CCT5 22948
Affinity Capture-MS Homo sapiens
48 PRPS2 5634
Affinity Capture-MS Homo sapiens
49 NOP2 4839
Affinity Capture-MS Homo sapiens
50 DIABLO 56616
Affinity Capture-Western Homo sapiens
51 LANCL2 55915
Affinity Capture-MS Homo sapiens
52 YWHAE 7531
Affinity Capture-MS Homo sapiens
53 RPL13A 23521
Affinity Capture-MS Homo sapiens
54 DNAJA1 3301
Affinity Capture-MS Homo sapiens
55 DCAF10  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
56 UBC 7316
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
57 YWHAQ 10971
Affinity Capture-MS Homo sapiens
58 RPN1 6184
Affinity Capture-MS Homo sapiens
59 TECR 9524
Affinity Capture-MS Homo sapiens
60 SND1 27044
Affinity Capture-MS Homo sapiens
61 VDAC2 7417
Affinity Capture-MS Homo sapiens
62 SEC61B 10952
Affinity Capture-MS Homo sapiens
63 ATP5B 506
Affinity Capture-MS Homo sapiens
64 H3F3C 440093
Affinity Capture-MS Homo sapiens
65 ACBD3 64746
Affinity Capture-MS Homo sapiens
66 RSL1D1 26156
Affinity Capture-MS Homo sapiens
67 MAT2A 4144
Affinity Capture-MS Homo sapiens
68 CAD 790
Affinity Capture-MS Homo sapiens
69 IP6K2  
Affinity Capture-MS Homo sapiens
70 TMPO 7112
Affinity Capture-MS Homo sapiens
71 IBA57  
Affinity Capture-MS Homo sapiens
72 AIFM1 9131
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
73 RPL23 9349
Affinity Capture-MS Homo sapiens
74 POLR2H 5437
Affinity Capture-MS Homo sapiens
75 XPO1 7514
Affinity Capture-MS Homo sapiens
76 YTHDF1 54915
Affinity Capture-MS Homo sapiens
77 EIF4G1 1981
Affinity Capture-MS Homo sapiens
78 AKT3  
Affinity Capture-Western Homo sapiens
79 TUBB4B 10383
Affinity Capture-MS Homo sapiens
80 AAAS 8086
Affinity Capture-MS Homo sapiens
81 PPIA 5478
Affinity Capture-MS Homo sapiens
82 RBBP4 5928
Affinity Capture-MS Homo sapiens
83 ATP5J2 9551
Affinity Capture-MS Homo sapiens
84 UMPS 7372
Affinity Capture-MS Homo sapiens
85 RPL17 6139
Affinity Capture-MS Homo sapiens
86 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
87 FLNB 2317
Affinity Capture-MS Homo sapiens
88 DHX30 22907
Affinity Capture-MS Homo sapiens
89 DHRS7B 25979
Affinity Capture-MS Homo sapiens
90 AKT2 208
Affinity Capture-Western Homo sapiens
91 HSPA4 3308
Affinity Capture-MS Homo sapiens
92 SORBS3 10174
Affinity Capture-MS Homo sapiens
93 DDB1 1642
Affinity Capture-Western Homo sapiens
94 NDUFA10 4705
Affinity Capture-MS Homo sapiens
95 CSDE1 7812
Affinity Capture-MS Homo sapiens
96 AKT1 207
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
97 BRD9 65980
Affinity Capture-MS Homo sapiens
98 PPP2CB 5516
Affinity Capture-MS Homo sapiens
99 TOMM20 9804
Affinity Capture-Western Homo sapiens
100 PHGDH 26227
Affinity Capture-MS Homo sapiens
101 PPIF 10105
Affinity Capture-MS Homo sapiens
102 EIF2S1 1965
Affinity Capture-MS Homo sapiens
103 SLC25A10 1468
Affinity Capture-MS Homo sapiens
104 FHL1 2273
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
105 CCT8 10694
Affinity Capture-MS Homo sapiens
106 SLC25A6 293
Affinity Capture-MS Homo sapiens
107 SMAD7  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
108 TP53 7157
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
109 PDCD2L 84306
Affinity Capture-MS Homo sapiens
110 IGLL5 100423062
Affinity Capture-MS Homo sapiens
111 FXR1 8087
Affinity Capture-MS Homo sapiens
112 MCL1 4170
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
113 KEAP1 9817
Affinity Capture-MS Homo sapiens
114 HSPB1 3315
Affinity Capture-MS Homo sapiens
115 CAPRIN1 4076
Affinity Capture-MS Homo sapiens
116 MTHFD1 4522
Affinity Capture-MS Homo sapiens
117 TARS 6897
Affinity Capture-MS Homo sapiens
118 CDK1 983
Affinity Capture-MS Homo sapiens
119 TRIM25 7706
Affinity Capture-MS Homo sapiens
120 PFKM 5213
Affinity Capture-MS Homo sapiens
121 ATAD3A 55210
Affinity Capture-MS Homo sapiens
122 DDX39A 10212
Affinity Capture-MS Homo sapiens
123 MCM5 4174
Affinity Capture-MS Homo sapiens
124 RPS24 6229
Affinity Capture-MS Homo sapiens
125 FARSA 2193
Affinity Capture-MS Homo sapiens
126 RTCB 51493
Affinity Capture-MS Homo sapiens
127 YBX3 8531
Affinity Capture-MS Homo sapiens
128 FLNA 2316
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
129 PRDX1 5052
Affinity Capture-MS Homo sapiens
130 RPS7 6201
Affinity Capture-MS Homo sapiens
131 SEC23B 10483
Affinity Capture-MS Homo sapiens
132 FLNC 2318
Affinity Capture-MS Homo sapiens
133 XRCC6 2547
Affinity Capture-MS Homo sapiens
134 SNW1 22938
Affinity Capture-MS Homo sapiens
135 GNB2L1 10399
Affinity Capture-MS Homo sapiens
136 PPP2R2A 5520
Affinity Capture-MS Homo sapiens
137 IRS4 8471
Affinity Capture-MS Homo sapiens
138 PRMT1 3276
Affinity Capture-MS Homo sapiens
139 CUL4A 8451
Affinity Capture-Western Homo sapiens
140 CDKN2A 1029
Affinity Capture-MS Homo sapiens
141 CDC5L 988
Affinity Capture-MS Homo sapiens
142 PSMD3 5709
Affinity Capture-MS Homo sapiens
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