Gene description for EIF4G1
Gene name eukaryotic translation initiation factor 4 gamma, 1
Gene symbol EIF4G1
Other names/aliases EIF-4G1
EIF4F
EIF4G
EIF4GI
P220
PARK18
Species Homo sapiens
 Database cross references - EIF4G1
ExoCarta ExoCarta_1981
Entrez Gene 1981
HGNC 3296
MIM 600495
UniProt Q04637  
 EIF4G1 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Saliva 19199708    
 Gene ontology annotations for EIF4G1
Molecular Function
    translation initiation factor activity GO:0003743 TAS
    protein binding GO:0005515 IPI
    translation factor activity, RNA binding GO:0008135 TAS
    poly(A) RNA binding GO:0044822 IDA
Biological Process
    cellular protein metabolic process GO:0044267 TAS
    regulation of translational initiation GO:0006446 NAS
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 TAS
    nuclear-transcribed mRNA poly(A) tail shortening GO:0000289 TAS
    insulin receptor signaling pathway GO:0008286 TAS
    nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay GO:0000288 TAS
    translational initiation GO:0006413 TAS
    gene expression GO:0010467 TAS
    cytokine-mediated signaling pathway GO:0019221 TAS
    translation GO:0006412 TAS
    viral process GO:0016032 IEA
Subcellular Localization
    cytosol GO:0005829 TAS
    eukaryotic translation initiation factor 4F complex GO:0016281 TAS
    membrane GO:0016020 IDA
 Experiment description of studies that identified EIF4G1 in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 66
ISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for EIF4G1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EIF4A2 1974
Affinity Capture-MS Homo sapiens
2 NELFCD 51497
Affinity Capture-MS Homo sapiens
3 EIF3A 8661
Affinity Capture-Western Homo sapiens
4 MAGEA6  
Affinity Capture-MS Homo sapiens
5 MKNK2 2872
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
6 EIF3B 8662
Affinity Capture-Western Homo sapiens
7 PDCD4 27250
Affinity Capture-Western Homo sapiens
8 GABARAPL2 11345
Affinity Capture-MS Homo sapiens
9 NCBP1 4686
Invivo Homo sapiens
10 Eif4e  
Affinity Capture-Western Mus musculus
11 PABPC1 26986
Invivo Homo sapiens
Invitro Homo sapiens
12 Eif4e 117045
Affinity Capture-Western Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
13 EIF4A1 1973
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
14 EIF4E 1977
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
15 MKNK1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
16 EIF3I 8668
Affinity Capture-Western Homo sapiens
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