Gene description for ATPAF2
Gene name ATP synthase mitochondrial F1 complex assembly factor 2
Gene symbol ATPAF2
Other names/aliases ATP12
ATP12p
LP3663
MC5DN1
Species Homo sapiens
 Database cross references - ATPAF2
ExoCarta ExoCarta_91647
Vesiclepedia VP_91647
Entrez Gene 91647
HGNC 18802
MIM 608918
UniProt Q8N5M1  
 ATPAF2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for ATPAF2
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    mitochondrial proton-transporting ATP synthase complex assembly GO:0033615 IBA
    mitochondrial proton-transporting ATP synthase complex assembly GO:0033615 IGI
    proton-transporting ATP synthase complex assembly GO:0043461 NAS
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrial inner membrane GO:0005743 IGI
    cytosol GO:0005829 IDA
    nuclear speck GO:0016607 IDA
 Experiment description of studies that identified ATPAF2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ATPAF2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CRTC2 200186
Two-hybrid Homo sapiens
2 RAD54B  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
3 BEGAIN  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
4 REL 5966
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
5 MCCC1 56922
Affinity Capture-MS Homo sapiens
6 APP 351
Reconstituted Complex Homo sapiens
7 C7orf55  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 RBM48  
Two-hybrid Homo sapiens
9 KRTAP19-7  
Two-hybrid Homo sapiens
10 HINT2 84681
Affinity Capture-MS Homo sapiens
11 FAM166B  
Two-hybrid Homo sapiens
12 CDK2AP1  
Two-hybrid Homo sapiens
13 YARS2  
Affinity Capture-MS Homo sapiens
14 KRT40  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
15 MAGEA6  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
16 EWSR1 2130
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
17 WASH1 100287171
Two-hybrid Homo sapiens
18 MIF4GD 57409
Two-hybrid Homo sapiens
19 LURAP1L  
Two-hybrid Homo sapiens
20 MEDAG  
Two-hybrid Homo sapiens
21 ZNF35  
Two-hybrid Homo sapiens
22 CEP57  
Two-hybrid Homo sapiens
23 NDUFAB1 4706
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 EBF1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
25 POLR2J3  
Two-hybrid Homo sapiens
26 GOLGA6L9  
Two-hybrid Homo sapiens
27 SPG21 51324
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 ATP5A1 498
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
29 DNLZ  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 DPYSL4 10570
Affinity Capture-MS Homo sapiens
31 ACSM5 54988
Affinity Capture-MS Homo sapiens
32 SORBS2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
33 OXLD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 PPP1R16B  
Two-hybrid Homo sapiens
35 POLR3F  
Two-hybrid Homo sapiens
36 LRRFIP2 9209
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
37 KRTAP6-2  
Two-hybrid Homo sapiens
38 FAM124A  
Affinity Capture-MS Homo sapiens
39 CYSRT1 375791
Two-hybrid Homo sapiens
40 ZNF23  
Two-hybrid Homo sapiens
41 C11orf1  
Two-hybrid Homo sapiens
42 TCHP  
Two-hybrid Homo sapiens
43 CABP1 9478
Affinity Capture-MS Homo sapiens
44 FCHSD2  
Two-hybrid Homo sapiens
45 ZNF655  
Two-hybrid Homo sapiens
46 HMGCL 3155
Affinity Capture-MS Homo sapiens
47 EBF3  
Two-hybrid Homo sapiens
48 PAK7  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
49 RASL10B  
Affinity Capture-MS Homo sapiens
50 ATP5B 506
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
51 C6orf165  
Two-hybrid Homo sapiens
52 MRM1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 COX5B 1329
Affinity Capture-MS Homo sapiens
54 KRTAP19-5  
Two-hybrid Homo sapiens
55 EIF4G1 1981
Two-hybrid Homo sapiens
56 UBAP2 55833
Two-hybrid Homo sapiens
57 IKZF3  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
58 LGALS9B 284194
Two-hybrid Homo sapiens
59 VWC2  
Two-hybrid Homo sapiens
60 ECHS1 1892
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 LZTS2 84445
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
62 LRTM1  
Affinity Capture-MS Homo sapiens
63 CTIF  
Two-hybrid Homo sapiens
64 FDPS 2224
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 PKP4 8502
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
66 TRIM27  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
67 FECH 2235
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 FNTB 2342
Two-hybrid Homo sapiens
69 PPP1R32  
Two-hybrid Homo sapiens
70 RPIA 22934
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
71 KRTAP6-3  
Two-hybrid Homo sapiens
72 ZNF426  
Two-hybrid Homo sapiens
73 CLPP 8192
Proximity Label-MS Homo sapiens
74 RPH3AL 9501
Two-hybrid Homo sapiens
75 TP53BP2  
Two-hybrid Homo sapiens
76 CLPX 10845
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 PRDM14  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
78 NME4 4833
Two-hybrid Homo sapiens
79 ZNF19  
Two-hybrid Homo sapiens
80 INCA1  
Two-hybrid Homo sapiens
81 FGB 2244
Affinity Capture-MS Homo sapiens
82 TRMT61B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 MAP10  
Affinity Capture-MS Homo sapiens
84 C21orf33  
Proximity Label-MS Homo sapiens
85 ZFP90  
Two-hybrid Homo sapiens
86 DDIT3 1649
Two-hybrid Homo sapiens
87 RNMTL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 KRTAP19-6  
Two-hybrid Homo sapiens
89 TRIM23 373
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
90 SPATC1L  
Two-hybrid Homo sapiens
91 KLHL22 84861
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 KRT34  
Two-hybrid Homo sapiens
93 LNX1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which ATPAF2 is involved
No pathways found





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