Gene description for ACSM5
Gene name acyl-CoA synthetase medium-chain family member 5
Gene symbol ACSM5
Other names/aliases -
Species Homo sapiens
 Database cross references - ACSM5
ExoCarta ExoCarta_54988
Vesiclepedia VP_54988
Entrez Gene 54988
HGNC 26060
MIM 614361
UniProt Q6NUN0  
 ACSM5 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for ACSM5
Molecular Function
    fatty-acyl-CoA synthase activity GO:0004321 IBA
    ATP binding GO:0005524 IEA
    GTP binding GO:0005525 IEA
    fatty acid ligase activity GO:0015645 IBA
    medium-chain fatty acid-CoA ligase activity GO:0031956 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    fatty acid biosynthetic process GO:0006633 IBA
    acyl-CoA metabolic process GO:0006637 IBA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrial matrix GO:0005759 IBA
 Experiment description of studies that identified ACSM5 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ACSM5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GLS 2744
Affinity Capture-MS Homo sapiens
2 CPT2 1376
Affinity Capture-MS Homo sapiens
3 ACSF2 80221
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 NR2F6  
Affinity Capture-MS Homo sapiens
5 PDPR 55066
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 BCKDK 10295
Affinity Capture-MS Homo sapiens
7 ATPAF1  
Affinity Capture-MS Homo sapiens
8 ALDH4A1 8659
Affinity Capture-MS Homo sapiens
9 HSPD1 3329
Affinity Capture-MS Homo sapiens
10 ADCK4 79934
Affinity Capture-MS Homo sapiens
11 MTO1  
Affinity Capture-MS Homo sapiens
12 LARS2 23395
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 UBR4 23352
Affinity Capture-MS Homo sapiens
14 RSAD1  
Affinity Capture-MS Homo sapiens
15 MTG2  
Affinity Capture-MS Homo sapiens
16 ACAD10  
Affinity Capture-MS Homo sapiens
17 PPOX 5498
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 FDXR 2232
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 CLUH 23277
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 HARS2 23438
Affinity Capture-MS Homo sapiens
21 MUT 4594
Affinity Capture-MS Homo sapiens
22 NUDT19 390916
Affinity Capture-MS Homo sapiens
23 TMLHE 55217
Affinity Capture-MS Homo sapiens
24 NLRX1 79671
Affinity Capture-MS Homo sapiens
25 CPS1 1373
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 CRAT 1384
Affinity Capture-MS Homo sapiens
27 ATPAF2 91647
Affinity Capture-MS Homo sapiens
28 TBRG4 9238
Affinity Capture-MS Homo sapiens
29 PCCA 5095
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 NDUFA2 4695
Affinity Capture-MS Homo sapiens
31 IARS2 55699
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 PCCB 5096
Affinity Capture-MS Homo sapiens
33 EARS2  
Affinity Capture-MS Homo sapiens
34 MIPEP  
Affinity Capture-MS Homo sapiens
35 VWA8 23078
Affinity Capture-MS Homo sapiens
36 TARS2 80222
Affinity Capture-MS Homo sapiens
37 ACSF3 197322
Affinity Capture-MS Homo sapiens
38 CYP24A1  
Affinity Capture-MS Homo sapiens
39 YARS2  
Affinity Capture-MS Homo sapiens
40 KIAA0391  
Affinity Capture-MS Homo sapiens
41 SERPINA10 51156
Affinity Capture-MS Homo sapiens
42 NARS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 AARS2  
Affinity Capture-MS Homo sapiens
44 POLG  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 MARS2 92935
Affinity Capture-MS Homo sapiens
46 MCCC1 56922
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 METTL15  
Affinity Capture-MS Homo sapiens
48 NSUN4  
Affinity Capture-MS Homo sapiens
49 PTCD1 26024
Affinity Capture-MS Homo sapiens
50 SARDH 1757
Affinity Capture-MS Homo sapiens
51 EP300 2033
Two-hybrid Homo sapiens
52 DARS2 55157
Affinity Capture-MS Homo sapiens
53 MTHFD1L 25902
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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