Gene description for ACSF2
Gene name acyl-CoA synthetase family member 2
Gene symbol ACSF2
Other names/aliases ACSMW
AVYV493
Species Homo sapiens
 Database cross references - ACSF2
ExoCarta ExoCarta_80221
Vesiclepedia VP_80221
Entrez Gene 80221
HGNC 26101
MIM 610465
UniProt Q96CM8  
 ACSF2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for ACSF2
Molecular Function
    long-chain fatty acid-CoA ligase activity GO:0004467 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    medium-chain fatty acid-CoA ligase activity GO:0031956 IBA
    medium-chain fatty acid-CoA ligase activity GO:0031956 IDA
Biological Process
    long-chain fatty acid metabolic process GO:0001676 IEA
    fatty acid metabolic process GO:0006631 IBA
    acyl-CoA metabolic process GO:0006637 TAS
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrial matrix GO:0005759 TAS
 Experiment description of studies that identified ACSF2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ACSF2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RAMP3  
Affinity Capture-MS Homo sapiens
2 UQCRFS1 7386
Affinity Capture-MS Homo sapiens
3 GCSH 2653
Co-fractionation Homo sapiens
4 ARHGEF26 26084
Affinity Capture-MS Homo sapiens
5 RPA2 6118
Proximity Label-MS Homo sapiens
6 MTG2  
Affinity Capture-MS Homo sapiens
7 BPNT1 10380
Affinity Capture-MS Homo sapiens
8 ARL6IP1 23204
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
9 APEX1 328
Affinity Capture-RNA Homo sapiens
10 APOC1  
Two-hybrid Homo sapiens
11 PPTC7 160760
Affinity Capture-MS Homo sapiens
12 TRMU  
Affinity Capture-MS Homo sapiens
13 CCS 9973
Co-fractionation Homo sapiens
14 MRPL23 6150
Affinity Capture-MS Homo sapiens
15 FAM174A 345757
Affinity Capture-MS Homo sapiens
16 YARS2  
Affinity Capture-MS Homo sapiens
17 MRPS24 64951
Affinity Capture-MS Homo sapiens
18 MRPL12 6182
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 GCAT  
Affinity Capture-MS Homo sapiens
20 FTSJ2 29960
Affinity Capture-MS Homo sapiens
21 DGAT2L6  
Two-hybrid Homo sapiens
22 FDX1L  
Co-fractionation Homo sapiens
23 NDUFAB1 4706
Co-fractionation Homo sapiens
24 SIGLEC12  
Two-hybrid Homo sapiens
25 PRTN3 5657
Affinity Capture-MS Homo sapiens
26 GSTO1 9446
Co-fractionation Homo sapiens
27 RDH10 157506
Two-hybrid Homo sapiens
28 TAZ  
Affinity Capture-MS Homo sapiens
29 BSG 682
Affinity Capture-MS Homo sapiens
30 SLC25A10 1468
Affinity Capture-MS Homo sapiens
31 SENP2 59343
Two-hybrid Homo sapiens
32 ACSM5 54988
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 PCK2 5106
Co-fractionation Homo sapiens
34 SUCLG1 8802
Co-fractionation Homo sapiens
35 PARK2  
Affinity Capture-MS Homo sapiens
36 AMACR 23600
Affinity Capture-MS Homo sapiens
37 APOA5 116519
Two-hybrid Homo sapiens
38 AGTRAP 57085
Two-hybrid Homo sapiens
39 DHRS2 10202
Affinity Capture-MS Homo sapiens
40 SLC6A20  
Affinity Capture-MS Homo sapiens
41 NIT1 4817
Affinity Capture-MS Homo sapiens
42 KDELC2 143888
Co-fractionation Homo sapiens
43 TPI1 7167
Co-fractionation Homo sapiens
44 NUDT16 131870
Affinity Capture-MS Homo sapiens
45 F12  
Affinity Capture-MS Homo sapiens
46 CREB3  
Two-hybrid Homo sapiens
47 GML  
Affinity Capture-MS Homo sapiens
48 PPA2 27068
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 ACTB 60
Co-fractionation Homo sapiens
50 DOCK10 55619
Co-fractionation Homo sapiens
51 ARPC5 10092
Co-fractionation Homo sapiens
52 ARL13B 200894
Two-hybrid Homo sapiens
53 GMPS 8833
Co-fractionation Homo sapiens
54 RASL10B  
Affinity Capture-MS Homo sapiens
55 LDHAL6B 92483
Affinity Capture-MS Homo sapiens
56 C10orf67  
Two-hybrid Homo sapiens
57 MRPL21 219927
Affinity Capture-MS Homo sapiens
58 AK4 205
Affinity Capture-MS Homo sapiens
59 NAA38  
Affinity Capture-MS Homo sapiens
60 PITRM1 10531
Co-fractionation Homo sapiens
61 ARPC1B 10095
Co-fractionation Homo sapiens
62 C2orf47  
Affinity Capture-MS Homo sapiens
63 FAHD1 81889
Affinity Capture-MS Homo sapiens
64 AQP3  
Affinity Capture-MS Homo sapiens
65 GPR152  
Two-hybrid Homo sapiens
66 PMPCB 9512
Co-fractionation Homo sapiens
67 GNB2 2783
Affinity Capture-MS Homo sapiens
68 GPR45  
Affinity Capture-MS Homo sapiens
69 CMTM5  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
70 C19orf70  
Two-hybrid Homo sapiens
71 WDR44 54521
Affinity Capture-MS Homo sapiens
72 HSPD1 3329
Affinity Capture-MS Homo sapiens
73 NIPSNAP3A  
Affinity Capture-MS Homo sapiens
74 APOA1BP 128240
Co-fractionation Homo sapiens
75 TSFM 10102
Co-fractionation Homo sapiens
76 SUCLG2 8801
Co-fractionation Homo sapiens
77 COMMD5 28991
Affinity Capture-MS Homo sapiens
78 ACAA2 10449
Affinity Capture-MS Homo sapiens
79 NDUFS7 374291
Affinity Capture-MS Homo sapiens
80 DDX58 23586
Affinity Capture-RNA Homo sapiens
81 NIPSNAP3B 55335
Affinity Capture-MS Homo sapiens
82 CLEC10A  
Two-hybrid Homo sapiens
83 GZMH  
Affinity Capture-MS Homo sapiens
84 SYNGR3  
Two-hybrid Homo sapiens
85 PCCB 5096
Affinity Capture-MS Homo sapiens
86 ANXA5 308
Co-fractionation Homo sapiens
87 SERBP1 26135
Affinity Capture-MS Homo sapiens
88 TRIM43  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ACSF2 is involved
PathwayEvidenceSource
Fatty acid metabolism TAS Reactome
Metabolism TAS Reactome
Metabolism of lipids TAS Reactome
Mitochondrial Fatty Acid Beta-Oxidation TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here