Gene description for NIT1
Gene name nitrilase 1
Gene symbol NIT1
Other names/aliases -
Species Homo sapiens
 Database cross references - NIT1
ExoCarta ExoCarta_4817
Vesiclepedia VP_4817
Entrez Gene 4817
HGNC 7828
MIM 604618
UniProt Q86X76  
 NIT1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for NIT1
Molecular Function
    deaminated glutathione amidase activity GO:0110050 ISS
Biological Process
    amide catabolic process GO:0043605 ISS
Subcellular Localization
    nucleus GO:0005634 HDA
    cytoplasm GO:0005737 ISS
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 ISS
 Experiment description of studies that identified NIT1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NIT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TUBA4A 7277
Affinity Capture-MS Homo sapiens
2 ETFA 2108
Co-fractionation Homo sapiens
3 ECH1 1891
Affinity Capture-MS Homo sapiens
4 HDAC4  
Affinity Capture-MS Homo sapiens
5 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
6 FASN 2194
Negative Genetic Homo sapiens
7 NDUFA11 126328
Affinity Capture-MS Homo sapiens
8 ACSF2 80221
Affinity Capture-MS Homo sapiens
9 FTSJ2 29960
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 CLPX 10845
Affinity Capture-MS Homo sapiens
11 ZFYVE9  
Two-hybrid Homo sapiens
12 NUDT9 53343
Co-fractionation Homo sapiens
13 IBA57  
Affinity Capture-MS Homo sapiens
14 BCAT2 587
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 POLG  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 PITRM1 10531
Affinity Capture-MS Homo sapiens
17 PDAP1 11333
Affinity Capture-MS Homo sapiens
18 RTP2  
Affinity Capture-MS Homo sapiens
19 ATXN1L  
Two-hybrid Homo sapiens
20 LARS2 23395
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 ISOC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 NF2 4771
Affinity Capture-MS Homo sapiens
23 TAZ  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 CD2AP 23607
Co-fractionation Homo sapiens
25 BOLA3  
Affinity Capture-MS Homo sapiens
26 PSMG4  
Co-fractionation Homo sapiens
27 ADI1 55256
Co-fractionation Homo sapiens
28 FPGS 2356
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 FDXR 2232
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 SLC25A27  
Affinity Capture-MS Homo sapiens
31 TOP3A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 NUDT19 390916
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 NUP35 129401
Proximity Label-MS Homo sapiens
34 NIPSNAP3A  
Affinity Capture-MS Homo sapiens
35 C17orf80 55028
Affinity Capture-MS Homo sapiens
36 IDE 3416
Affinity Capture-MS Homo sapiens
37 HOXC5  
Affinity Capture-MS Homo sapiens
38 PMPCB 9512
Affinity Capture-MS Homo sapiens
39 LOC102724023  
Affinity Capture-MS Homo sapiens
40 C1QBP 708
Affinity Capture-MS Homo sapiens
41 SLC25A20 788
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 NUDT8  
Affinity Capture-MS Homo sapiens
43 IFT74 80173
Affinity Capture-MS Homo sapiens
44 PMPCA 23203
Affinity Capture-MS Homo sapiens
45 CAMK2A 815
Affinity Capture-MS Homo sapiens
46 FECH 2235
Affinity Capture-MS Homo sapiens
47 SSSCA1 10534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 RPUSD3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 NIT1 4817
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 GLRX 2745
Co-fractionation Homo sapiens
51 POLDIP2 26073
Affinity Capture-MS Homo sapiens
52 SHARPIN  
Affinity Capture-MS Homo sapiens
53 XRCC6 2547
Two-hybrid Homo sapiens
54 KIAA0391  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 CUX1 1523
Co-fractionation Homo sapiens
56 PDPR 55066
Affinity Capture-MS Homo sapiens
57 NARS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 HADHB 3032
Affinity Capture-MS Homo sapiens
59 PTS  
Co-fractionation Homo sapiens
60 SLC31A1 1317
Affinity Capture-MS Homo sapiens
61 HOGA1  
Affinity Capture-MS Homo sapiens
62 CAPN1 823
Co-fractionation Homo sapiens
63 BCKDK 10295
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 GTPBP8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 SIRT3  
Affinity Capture-MS Homo sapiens
66 NEDD8 4738
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which NIT1 is involved
No pathways found





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