Gene description for BCKDK
Gene name branched chain ketoacid dehydrogenase kinase
Gene symbol BCKDK
Other names/aliases BCKDKD
BDK
Species Homo sapiens
 Database cross references - BCKDK
ExoCarta ExoCarta_10295
Vesiclepedia VP_10295
Entrez Gene 10295
HGNC 16902
MIM 614901
UniProt O14874  
 BCKDK identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for BCKDK
Molecular Function
    protein serine/threonine kinase activity GO:0004674 ISS
    protein serine/threonine phosphatase activity GO:0004722 IDA
    pyruvate dehydrogenase (acetyl-transferring) kinase activity GO:0004740 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 ISS
    kinase activity GO:0016301 TAS
    [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] kinase activity GO:0047323 TAS
    protein serine kinase activity GO:0106310 IEA
Biological Process
    lipid biosynthetic process GO:0008610 IMP
    amino acid catabolic process GO:0009063 NAS
    branched-chain amino acid catabolic process GO:0009083 ISS
    branched-chain amino acid catabolic process GO:0009083 TAS
    regulation of acetyl-CoA biosynthetic process from pyruvate GO:0010510 IBA
    regulation of glucose metabolic process GO:0010906 IBA
    negative regulation of amino acid metabolic process GO:0045763 TAS
Subcellular Localization
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 TAS
    mitochondrial matrix GO:0005759 TAS
    oxoglutarate dehydrogenase complex GO:0045252 ISS
    oxoglutarate dehydrogenase complex GO:0045252 TAS
 Experiment description of studies that identified BCKDK in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for BCKDK
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATG16L1 55054
Affinity Capture-MS Homo sapiens
2 GCNT3 9245
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 NIPSNAP3A  
Affinity Capture-MS Homo sapiens
4 CETN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 SSBP1 6742
Proximity Label-MS Homo sapiens
6 MECP2 4204
Affinity Capture-MS Homo sapiens
7 UQCRFS1 7386
Affinity Capture-MS Homo sapiens
8 CPSF6 11052
Affinity Capture-MS Homo sapiens
9 TP53BP1 7158
Affinity Capture-MS Homo sapiens
10 RBM39 9584
Affinity Capture-MS Homo sapiens
11 TERF2  
Affinity Capture-MS Homo sapiens
12 RNF219  
Affinity Capture-MS Homo sapiens
13 AK4 205
Affinity Capture-MS Homo sapiens
14 CUL3 8452
Affinity Capture-MS Homo sapiens
15 CAB39 51719
Affinity Capture-MS Homo sapiens
16 FOXRED2  
Affinity Capture-MS Homo sapiens
17 MTIF2 4528
Proximity Label-MS Homo sapiens
18 C20orf96  
Affinity Capture-MS Homo sapiens
19 CETN3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 WDTC1 23038
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 HCST  
Affinity Capture-MS Homo sapiens
22 DYNC1I2 1781
Proximity Label-MS Homo sapiens
23 TAZ  
Affinity Capture-MS Homo sapiens
24 BSG 682
Affinity Capture-MS Homo sapiens
25 CETN2 1069
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 BCKDHA 593
Affinity Capture-MS Homo sapiens
27 IGHM 3507
Affinity Capture-MS Homo sapiens
28 PDHA1 5160
Proximity Label-MS Homo sapiens
29 RASL10B  
Affinity Capture-MS Homo sapiens
30 MAP4 4134
Affinity Capture-MS Homo sapiens
31 ACSM5 54988
Affinity Capture-MS Homo sapiens
32 EPS8 2059
Two-hybrid Homo sapiens
33 NDUFS7 374291
Affinity Capture-MS Homo sapiens
34 FAHD1 81889
Affinity Capture-MS Homo sapiens
35 BPNT1 10380
Affinity Capture-MS Homo sapiens
36 MRPL52  
Affinity Capture-MS Homo sapiens
37 OBFC1 79991
Co-fractionation Homo sapiens
38 CAB39L 81617
Affinity Capture-MS Homo sapiens
39 AMACR 23600
Affinity Capture-MS Homo sapiens
40 FAM174A 345757
Affinity Capture-MS Homo sapiens
41 ARMC8 25852
Affinity Capture-MS Homo sapiens
42 APP 351
Reconstituted Complex Homo sapiens
43 TNS2 23371
Affinity Capture-MS Homo sapiens
44 SNX9 51429
Affinity Capture-MS Homo sapiens
45 NR5A2  
Two-hybrid Homo sapiens
46 LGALS3BP 3959
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 RTCA 8634
Affinity Capture-MS Homo sapiens
48 MAPK6  
Affinity Capture-MS Homo sapiens
49 HNRNPH1 3187
Affinity Capture-MS Homo sapiens
50 FBXW7  
Affinity Capture-MS Homo sapiens
51 FTSJ2 29960
Affinity Capture-MS Homo sapiens
52 TJP2 9414
Affinity Capture-MS Homo sapiens
53 SSC4D  
Affinity Capture-MS Homo sapiens
54 TRMU  
Affinity Capture-MS Homo sapiens
55 MTG2  
Affinity Capture-MS Homo sapiens
56 PARK2  
Affinity Capture-MS Homo sapiens
57 HECTD1 25831
Affinity Capture-MS Homo sapiens
58 OXCT2  
Affinity Capture-MS Homo sapiens
59 METTL21C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 CBR4  
Affinity Capture-MS Homo sapiens
61 LSM8 51691
Two-hybrid Homo sapiens
62 NUDT16 131870
Affinity Capture-MS Homo sapiens
63 EXD2  
Proximity Label-MS Homo sapiens
64 NIT1 4817
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 MYL10 93408
Affinity Capture-MS Homo sapiens
66 PPM1K  
Affinity Capture-MS Homo sapiens
67 CLEC11A 6320
Affinity Capture-MS Homo sapiens
68 YARS2  
Affinity Capture-MS Homo sapiens
69 MRPL12 6182
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 GCAT  
Affinity Capture-MS Homo sapiens
71 NDUFA10 4705
Affinity Capture-MS Homo sapiens
72 QRSL1  
Affinity Capture-MS Homo sapiens
73 ISLR 3671
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 HSPD1 3329
Affinity Capture-MS Homo sapiens
75 FAM98B 283742
Affinity Capture-MS Homo sapiens
76 STAT3 6774
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
77 DNAJB1 3337
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 TRIM43  
Affinity Capture-MS Homo sapiens
79 PFDN5 5204
Affinity Capture-MS Homo sapiens
80 PNKP 11284
Co-fractionation Homo sapiens
81 PPM1A 5494
Affinity Capture-MS Homo sapiens
82 SPTA1  
Two-hybrid Homo sapiens
83 SLC25A10 1468
Affinity Capture-MS Homo sapiens
84 SHC2 25759
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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