Gene description for PFDN5
Gene name prefoldin subunit 5
Gene symbol PFDN5
Other names/aliases MM-1
MM1
PFD5
Species Homo sapiens
 Database cross references - PFDN5
ExoCarta ExoCarta_5204
Entrez Gene 5204
HGNC 8869
MIM 604899
UniProt Q99471  
 PFDN5 identified in exosomes derived from the following tissue/cell type
Prostate cancer cells 25844599    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for PFDN5
Molecular Function
    unfolded protein binding GO:0051082 IEA
    protein binding GO:0005515 IPI
    transcription corepressor activity GO:0003714 TAS
Biological Process
    regulation of transcription, DNA-templated GO:0006355 TAS
    retina development in camera-type eye GO:0060041 IEA
    protein folding GO:0006457 TAS
    negative regulation of transcription, DNA-templated GO:0045892 IMP
    cellular protein metabolic process GO:0044267 TAS
    negative regulation of canonical Wnt signaling pathway GO:0090090 IMP
    'de novo' posttranslational protein folding GO:0051084 TAS
Subcellular Localization
    prefoldin complex GO:0016272 IEA
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 TAS
 Experiment description of studies that identified PFDN5 in exosomes
1
Experiment ID 275
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
2
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PFDN5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MYC  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
2 HKE2  
Co-purification Homo sapiens
View the network image/svg+xml
 Pathways in which PFDN5 is involved
PathwayEvidenceSource
Prefoldin mediated transfer of substrate to CCT/TriC IEA Reactome





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