Gene description for SAMM50
Gene name SAMM50 sorting and assembly machinery component
Gene symbol SAMM50
Other names/aliases CGI-51
OMP85
SAM50
TOB55
TRG-3
YNL026W
Species Homo sapiens
 Database cross references - SAMM50
ExoCarta ExoCarta_25813
Entrez Gene 25813
HGNC 24276
MIM 612058
UniProt Q9Y512  
 SAMM50 identified in exosomes derived from the following tissue/cell type
Urine 15326289    
Urine 19056867    
 Gene ontology annotations for SAMM50
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    protein import into mitochondrial outer membrane GO:0045040 IDA
    cellular protein metabolic process GO:0044267 TAS
    protein targeting to mitochondrion GO:0006626 TAS
Subcellular Localization
    mitochondrial sorting and assembly machinery complex GO:0001401 IMP
    mitochondrial inner membrane GO:0005743 IEA
    extracellular exosome GO:0070062 IDA
    mitochondrion GO:0005739 IDA
    mitochondrial outer membrane GO:0005741 IDA
    integral component of membrane GO:0016021 IDA
 Experiment description of studies that identified SAMM50 in exosomes
1
Experiment ID 13
ISEV standards
✔
IEM
EV Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101
EV Cytosolic markers
✔
CD9|AQP2|AQP1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors Pisitkun T, Shen RF, Knepper MA
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
2
Experiment ID 63
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for SAMM50
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MEOX2  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which SAMM50 is involved
PathwayEvidenceSource
Mitochondrial protein import TAS Reactome





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