Gene description for SLC22A8
Gene name solute carrier family 22 (organic anion transporter), member 8
Gene symbol SLC22A8
Other names/aliases OAT3
Species Homo sapiens
 Database cross references - SLC22A8
ExoCarta ExoCarta_9376
Entrez Gene 9376
HGNC 10972
MIM 607581
UniProt Q8TCC7  
 SLC22A8 identified in exosomes derived from the following tissue/cell type
Prostate cancer cells 25844599    
Urine 19056867    
 Gene ontology annotations for SLC22A8
Molecular Function
    inorganic anion exchanger activity GO:0005452 IDA
    quaternary ammonium group transmembrane transporter activity GO:0015651 IEA
    protein kinase C binding GO:0005080 IEA
    organic anion transmembrane transporter activity GO:0008514 IEA
Biological Process
    transmembrane transport GO:0055085 TAS
    glutathione transport GO:0034635 IEA
    anion transmembrane transport GO:0098656 IDA
    response to toxic substance GO:0009636 IEA
    response to methotrexate GO:0031427 IEA
    quaternary ammonium group transport GO:0015697 IEA
Subcellular Localization
    integral component of membrane GO:0016021 IEA
    plasma membrane GO:0005886 TAS
    extracellular exosome GO:0070062 IDA
    basolateral plasma membrane GO:0016323 IDA
 Experiment description of studies that identified SLC22A8 in exosomes
1
Experiment ID 274
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
2
Experiment ID 63
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for SLC22A8
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which SLC22A8 is involved
PathwayEvidenceSource
Organic anion transport TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here