Gene description for NDFIP1
Gene name Nedd4 family interacting protein 1
Gene symbol NDFIP1
Other names/aliases N4WBP5
Species Homo sapiens
 Database cross references - NDFIP1
ExoCarta ExoCarta_80762
Vesiclepedia VP_80762
Entrez Gene 80762
HGNC 17592
MIM 612050
UniProt Q9BT67  
 NDFIP1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for NDFIP1
Molecular Function
    protein binding GO:0005515 IPI
    WW domain binding GO:0050699 IEA
Biological Process
    regulation of myeloid leukocyte differentiation GO:0002761 IEA
    negative regulation of type 2 immune response GO:0002829 IEA
    ubiquitin-dependent protein catabolic process GO:0006511 IBA
    intracellular iron ion homeostasis GO:0006879 IMP
    vacuolar transport GO:0007034 IEA
    negative regulation of gene expression GO:0010629 IMP
    metal ion transport GO:0030001 IEA
    positive regulation of protein ubiquitination GO:0031398 IBA
    positive regulation of protein ubiquitination GO:0031398 IDA
    positive regulation of protein ubiquitination GO:0031398 IMP
    negative regulation of transporter activity GO:0032410 IMP
    negative regulation of interleukin-4 production GO:0032713 IEA
    CD4-positive, alpha-beta T cell proliferation GO:0035739 IEA
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 HMP
    regulation of lymphocyte differentiation GO:0045619 IEA
    positive regulation of protein catabolic process GO:0045732 IEA
    negative regulation of isotype switching to IgE isotypes GO:0048294 IEA
    regulation of isotype switching to IgG isotypes GO:0048302 IEA
    negative regulation of inflammatory response GO:0050728 IEA
    negative regulation of protein transport GO:0051224 IMP
    negative regulation of CD4-positive, alpha-beta T cell proliferation GO:2000562 IEA
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    extracellular region GO:0005576 IEA
    endoplasmic reticulum GO:0005783 IBA
    Golgi apparatus GO:0005794 IBA
    cell cortex GO:0005938 IEA
    endosome membrane GO:0010008 IEA
    dendrite GO:0030425 IEA
    synapse GO:0045202 IEA
    perinuclear region of cytoplasm GO:0048471 IBA
    perinuclear region of cytoplasm GO:0048471 IDA
 Experiment description of studies that identified NDFIP1 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NDFIP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RNF149 284996
Affinity Capture-MS Homo sapiens
2 PTGIR  
Affinity Capture-MS Homo sapiens
3 TMEM192 201931
Affinity Capture-MS Homo sapiens
4 LRRIQ1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 MAVS 57506
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
6 BACE2 25825
Affinity Capture-MS Homo sapiens
7 C19orf18 147685
Affinity Capture-MS Homo sapiens
8 OR10H1  
Affinity Capture-MS Homo sapiens
9 IZUMO1  
Affinity Capture-MS Homo sapiens
10 SLC22A8 9376
Affinity Capture-MS Homo sapiens
11 NEDD4 4734
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
12 OPN4 94233
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 TGFBR2 7048
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 PTPRN2  
Affinity Capture-MS Homo sapiens
15 OR10H2  
Affinity Capture-MS Homo sapiens
16 CTXN1  
Affinity Capture-MS Homo sapiens
17 WWP1 11059
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
18 MARCH1  
Affinity Capture-MS Homo sapiens
19 ARRDC4  
Affinity Capture-MS Homo sapiens
20 CD83  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 HCST  
Affinity Capture-MS Homo sapiens
22 UNK  
Affinity Capture-RNA Homo sapiens
23 ZDHHC7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 IL32  
Affinity Capture-MS Homo sapiens
25 LRRN2 10446
Affinity Capture-MS Homo sapiens
26 SMO 6608
Two-hybrid Homo sapiens
27 TCIRG1 10312
Affinity Capture-MS Homo sapiens
28 PIGH  
Affinity Capture-MS Homo sapiens
29 MANSC1 54682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 MYCT1 80177
Affinity Capture-MS Homo sapiens
31 NRSN1  
Affinity Capture-MS Homo sapiens
32 CX3CL1  
Affinity Capture-MS Homo sapiens
33 PTEN 5728
Affinity Capture-Western Homo sapiens
PCA Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
34 SMURF1 57154
Affinity Capture-Western Homo sapiens
35 ITCH 83737
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
36 EMC1 23065
Affinity Capture-MS Homo sapiens
37 IFNGR1 3459
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 SLC18A2  
Affinity Capture-MS Homo sapiens
39 SNCA 6622
Affinity Capture-Western Homo sapiens
40 KCNK4  
Affinity Capture-MS Homo sapiens
41 ENTPD7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 C16orf92  
Affinity Capture-MS Homo sapiens
43 TSPAN3 10099
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 PIGN 23556
Affinity Capture-MS Homo sapiens
45 HEPACAM2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 NDFIP2 54602
Affinity Capture-MS Homo sapiens
47 FCER1A  
Affinity Capture-MS Homo sapiens
48 UFL1 23376
Affinity Capture-MS Homo sapiens
49 ALG6  
Affinity Capture-MS Homo sapiens
50 WWP2 11060
Affinity Capture-MS Homo sapiens
51 TNFRSF10B 8795
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 BDKRB1 623
Two-hybrid Homo sapiens
53 AVPR1B  
Affinity Capture-MS Homo sapiens
54 SLC20A1 6574
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 SLC11A2 4891
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
56 C16orf58 64755
Affinity Capture-MS Homo sapiens
57 TMEM92  
Affinity Capture-MS Homo sapiens
58 EGFR 1956
Affinity Capture-Western Homo sapiens
59 FAM168A  
Affinity Capture-MS Homo sapiens
60 CCR2  
Two-hybrid Homo sapiens
61 FAM8A1 51439
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 GINM1 116254
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 TMEM231 79583
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 TNFRSF10A 8797
Affinity Capture-MS Homo sapiens
65 NEDD4L 23327
Affinity Capture-Western Homo sapiens
66 PTGFR 5737
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 STX11 8676
Affinity Capture-MS Homo sapiens
68 TPCN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 KCNK18  
Affinity Capture-MS Homo sapiens
70 IFNAR1  
Affinity Capture-MS Homo sapiens
71 SLC1A1 6505
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 UQCRH 7388
Two-hybrid Homo sapiens
73 TEX29  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 MRAP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 KCNC3 3748
Affinity Capture-MS Homo sapiens
76 C14orf37  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 IQCF3  
Affinity Capture-MS Homo sapiens
78 PRRG3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 DRD2  
Affinity Capture-MS Homo sapiens
80 EDAR  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NDFIP1 is involved
No pathways found





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