Gene description for PIGN
Gene name phosphatidylinositol glycan anchor biosynthesis, class N
Gene symbol PIGN
Other names/aliases MCAHS
MCAHS1
MCD4
MDC4
PIG-N
Species Homo sapiens
 Database cross references - PIGN
ExoCarta ExoCarta_23556
Vesiclepedia VP_23556
Entrez Gene 23556
HGNC 8967
MIM 606097
UniProt O95427  
 PIGN identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for PIGN
Molecular Function
    mannose-ethanolamine phosphotransferase activity GO:0051377 IBA
    mannose-ethanolamine phosphotransferase activity GO:0051377 TAS
Biological Process
    GPI anchor biosynthetic process GO:0006506 IBA
    GPI anchor biosynthetic process GO:0006506 IEA
    GPI anchor biosynthetic process GO:0006506 TAS
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 IBA
    endoplasmic reticulum membrane GO:0005789 TAS
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IDA
    membrane GO:0016020 HDA
 Experiment description of studies that identified PIGN in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PIGN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RNF149 284996
Affinity Capture-MS Homo sapiens
2 PTGIR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 LMBRD2 92255
Affinity Capture-MS Homo sapiens
4 TMEM87A 25963
Affinity Capture-MS Homo sapiens
5 FBXO45 200933
Affinity Capture-MS Homo sapiens
6 RHBDD1 84236
Affinity Capture-MS Homo sapiens
7 VIPR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 GPR89A  
Affinity Capture-MS Homo sapiens
9 FJX1 24147
Two-hybrid Homo sapiens
10 PTPRD 5789
Affinity Capture-MS Homo sapiens
11 CLGN 1047
Affinity Capture-MS Homo sapiens
12 CHRNA4  
Affinity Capture-MS Homo sapiens
13 TTYH1  
Affinity Capture-MS Homo sapiens
14 MYCBP2 23077
Affinity Capture-MS Homo sapiens
15 RXFP1  
Affinity Capture-MS Homo sapiens
16 KRTCAP2 200185
Affinity Capture-MS Homo sapiens
17 TXNDC15  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 LRRC59 55379
Proximity Label-MS Homo sapiens
19 OPALIN  
Affinity Capture-MS Homo sapiens
20 RNF181  
Affinity Capture-MS Homo sapiens
21 HCST  
Affinity Capture-MS Homo sapiens
22 NDFIP1 80762
Affinity Capture-MS Homo sapiens
23 FITM2  
Affinity Capture-MS Homo sapiens
24 KDELR1 10945
Affinity Capture-MS Homo sapiens
25 TSPAN15 23555
Affinity Capture-MS Homo sapiens
26 VKORC1L1 154807
Affinity Capture-MS Homo sapiens
27 GOLIM4 27333
Affinity Capture-MS Homo sapiens
28 SPSB4  
Affinity Capture-MS Homo sapiens
29 PTEN 5728
Synthetic Growth Defect Homo sapiens
30 GCGR  
Affinity Capture-MS Homo sapiens
31 TUBB4B 10383
Affinity Capture-MS Homo sapiens
32 TMEM126A 84233
Affinity Capture-MS Homo sapiens
33 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
34 PIGU 128869
Affinity Capture-MS Homo sapiens
35 UNC93B1 81622
Affinity Capture-MS Homo sapiens
36 SHMT2 6472
Affinity Capture-RNA Homo sapiens
37 SLC22A9  
Affinity Capture-MS Homo sapiens
38 CERS5 91012
Affinity Capture-MS Homo sapiens
39 NXF1 10482
Affinity Capture-RNA Homo sapiens
40 SCN4A  
Affinity Capture-MS Homo sapiens
41 RABL3 285282
Affinity Capture-MS Homo sapiens
42 PTDSS2 81490
Affinity Capture-MS Homo sapiens
43 MAOB  
Affinity Capture-MS Homo sapiens
44 MOV10 4343
Affinity Capture-RNA Homo sapiens
45 SPPL2B 56928
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 SCAMP2 10066
Affinity Capture-MS Homo sapiens
47 CMTM5  
Affinity Capture-MS Homo sapiens
48 ATP2B2 491
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 GPR21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 TMEM246 84302
Affinity Capture-MS Homo sapiens
51 TMEM9 252839
Affinity Capture-MS Homo sapiens
52 CERS6  
Affinity Capture-MS Homo sapiens
53 SYNGR2 9144
Affinity Capture-MS Homo sapiens
54 ENTPD2 954
Affinity Capture-MS Homo sapiens
55 TMEM63A 9725
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 YIPF3 25844
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 SIGMAR1 10280
Affinity Capture-MS Homo sapiens
58 CCR6  
Affinity Capture-MS Homo sapiens
59 BRI3BP 140707
Affinity Capture-MS Homo sapiens
60 FPR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 MALL  
Affinity Capture-MS Homo sapiens
62 SURF4 6836
Affinity Capture-MS Homo sapiens
63 UST  
Affinity Capture-MS Homo sapiens
64 AVPR2  
Affinity Capture-MS Homo sapiens
65 SNAP29 9342
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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