Gene description for CERS5
Gene name ceramide synthase 5
Gene symbol CERS5
Other names/aliases LASS5
Trh4
Species Homo sapiens
 Database cross references - CERS5
ExoCarta ExoCarta_91012
Vesiclepedia VP_91012
Entrez Gene 91012
HGNC 23749
MIM 615335
UniProt Q8N5B7  
 CERS5 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for CERS5
Molecular Function
    DNA binding GO:0003677 IEA
    sphingosine N-acyltransferase activity GO:0050291 IBA
    sphingosine N-acyltransferase activity GO:0050291 IDA
    sphingosine N-acyltransferase activity GO:0050291 TAS
Biological Process
    oligodendrocyte development GO:0014003 IEA
    sphingolipid biosynthetic process GO:0030148 TAS
    ceramide biosynthetic process GO:0046513 IBA
    ceramide biosynthetic process GO:0046513 IDA
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 TAS
 Experiment description of studies that identified CERS5 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CERS5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TACR3  
Affinity Capture-MS Homo sapiens
2 VIPR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 CXCR4 7852
Affinity Capture-MS Homo sapiens
4 TTYH1  
Affinity Capture-MS Homo sapiens
5 C3AR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 CD83  
Affinity Capture-MS Homo sapiens
7 NPTN 27020
Affinity Capture-MS Homo sapiens
8 BSCL2  
Affinity Capture-MS Homo sapiens
9 GCGR  
Affinity Capture-MS Homo sapiens
10 NIPAL1  
Affinity Capture-MS Homo sapiens
11 HMGCS1 3157
Affinity Capture-MS Homo sapiens
12 HTR2C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 LPAR1 1902
Affinity Capture-MS Homo sapiens
14 PIGN 23556
Affinity Capture-MS Homo sapiens
15 SEC62 7095
Affinity Capture-MS Homo sapiens
16 C16orf72 29035
Affinity Capture-MS Homo sapiens
17 CMTM5  
Affinity Capture-MS Homo sapiens
18 GPR182  
Affinity Capture-MS Homo sapiens
19 HCAR1  
Affinity Capture-MS Homo sapiens
20 IPPK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 CCR6  
Affinity Capture-MS Homo sapiens
22 TMEM57  
Affinity Capture-MS Homo sapiens
23 FPR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 HUWE1 10075
Affinity Capture-MS Homo sapiens
25 P2RY12 64805
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CERS5 is involved
PathwayEvidenceSource
Metabolism TAS Reactome
Metabolism of lipids TAS Reactome
Sphingolipid de novo biosynthesis TAS Reactome
Sphingolipid metabolism TAS Reactome





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