Gene description for P2RY12
Gene name purinergic receptor P2Y, G-protein coupled, 12
Gene symbol P2RY12
Other names/aliases ADPG-R
BDPLT8
HORK3
P2T(AC)
P2Y(12)R
P2Y(AC)
P2Y(ADP)
P2Y(cyc)
P2Y12
SP1999
Species Homo sapiens
 Database cross references - P2RY12
ExoCarta ExoCarta_64805
Vesiclepedia VP_64805
Entrez Gene 64805
HGNC 18124
MIM 600515
UniProt Q9H244  
 P2RY12 identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for P2RY12
Molecular Function
    G protein-coupled adenosine receptor activity GO:0001609 IEA
    G protein-coupled ADP receptor activity GO:0001621 IDA
    G protein-coupled ADP receptor activity GO:0001621 IMP
    guanyl-nucleotide exchange factor activity GO:0005085 TAS
    G protein-coupled purinergic nucleotide receptor activity GO:0045028 IBA
Biological Process
    G protein-coupled adenosine receptor signaling pathway GO:0001973 IEA
    monoatomic ion transport GO:0006811 IEA
    substrate-dependent cell migration, cell extension GO:0006930 ISS
    G protein-coupled receptor signaling pathway GO:0007186 IBA
    G protein-coupled receptor signaling pathway GO:0007186 IDA
    adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway GO:0007193 IMP
    phospholipase C-activating G protein-coupled receptor signaling pathway GO:0007200 ISS
    hemostasis GO:0007599 NAS
    calcium-mediated signaling GO:0019722 ISS
    cerebral cortex radial glia-guided migration GO:0021801 ISS
    cell projection organization GO:0030030 ISS
    lamellipodium assembly GO:0030032 ISS
    platelet activation GO:0030168 TAS
    positive regulation of integrin activation by cell surface receptor linked signal transduction GO:0033626 ISS
    positive regulation of cell adhesion mediated by integrin GO:0033630 ISS
    G protein-coupled purinergic nucleotide receptor signaling pathway GO:0035589 IEA
    positive regulation of monoatomic ion transport GO:0043270 IEA
    response to axon injury GO:0048678 ISS
    regulation of chemotaxis GO:0050920 ISS
    regulation of chemotaxis GO:0050920 TAS
    positive regulation of chemotaxis GO:0050921 ISS
    establishment of localization in cell GO:0051649 IEA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 ISS
    platelet aggregation GO:0070527 IMP
    cellular response to ATP GO:0071318 ISS
    visual system development GO:0150063 IEA
    positive regulation of ruffle assembly GO:1900029 ISS
    positive regulation of ruffle assembly GO:1900029 TAS
    regulation of microglial cell migration GO:1904139 ISS
    positive regulation of microglial cell migration GO:1904141 ISS
    positive regulation of microglial cell migration GO:1904141 TAS
Subcellular Localization
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 HDA
    membrane GO:0016020 IDA
    cell projection membrane GO:0031253 IEA
    cell body membrane GO:0044298 IEA
 Experiment description of studies that identified P2RY12 in exosomes
1
Experiment ID 231
MISEV standards
Biophysical techniques
Alix|CD63|CD9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for P2RY12
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CRELD1 78987
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 ATP5H 10476
Affinity Capture-MS Homo sapiens
3 CMTM6 54918
Affinity Capture-MS Homo sapiens
4 GPR89A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 ITPR2 3709
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 TMEM164  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 GEMIN8 54960
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 BLVRA 644
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 SACM1L 22908
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 TTYH3 80727
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 GOLPH3 64083
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 RNF170 81790
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 ARL6IP5 10550
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 FITM2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 KIAA2013 90231
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 APOB 338
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 LGALS3 3958
Affinity Capture-MS Homo sapiens
18 VKORC1L1 154807
Affinity Capture-MS Homo sapiens
19 IPO11 51194
Affinity Capture-MS Homo sapiens
20 NME2P1 283458
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 TSPAN15 23555
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 ATP5F1 515
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 RMND1 55005
Affinity Capture-MS Homo sapiens
24 TMEM161B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 MFSD8 256471
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 EBP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 C19orf25  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 SAAL1 113174
Affinity Capture-MS Homo sapiens
29 SLC39A1 27173
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 GEMIN6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 CERS5 91012
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 PTDSS2 81490
Affinity Capture-MS Homo sapiens
33 TM9SF3 56889
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 ACP2 53
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 GEMIN4 50628
Affinity Capture-MS Homo sapiens
36 XPO4 64328
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 DDX20 11218
Affinity Capture-MS Homo sapiens
38 ALG10  
Affinity Capture-MS Homo sapiens
39 RFT1 91869
Affinity Capture-MS Homo sapiens
40 GOLGA5 9950
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 ND1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 MRS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 TNPO3 23534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 PTRHD1 391356
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 COX1 4512
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 TMPPE  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 SLC30A7 148867
Affinity Capture-MS Homo sapiens
48 ACAD9 28976
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 CERS6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 MPP6 51678
Affinity Capture-MS Homo sapiens
51 YIPF3 25844
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 DYM  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 LMBR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 TTC39A 22996
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 CAV1 857
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 BRI3BP 140707
Affinity Capture-MS Homo sapiens
57 ARL10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 SLC5A3 6526
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 ATP5B 506
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 ECSIT 51295
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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