Gene description for ECSIT
Gene name ECSIT signalling integrator
Gene symbol ECSIT
Other names/aliases SITPEC
Species Homo sapiens
 Database cross references - ECSIT
ExoCarta ExoCarta_51295
Vesiclepedia VP_51295
Entrez Gene 51295
HGNC 29548
MIM 608388
UniProt Q9BQ95  
 ECSIT identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
 Gene ontology annotations for ECSIT
Molecular Function
    protein binding GO:0005515 IPI
    molecular adaptor activity GO:0060090 IDA
Biological Process
    cell surface receptor protein serine/threonine kinase signaling pathway GO:0007178 IBA
    mitochondrial respiratory chain complex I assembly GO:0032981 NAS
    toll-like receptor 4 signaling pathway GO:0034142 IDA
    innate immune response GO:0045087 IBA
    regulation of oxidoreductase activity GO:0051341 IDA
    regulation of protein complex stability GO:0061635 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IC
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial inner membrane GO:0005743 NAS
    mitochondrial inner membrane GO:0005743 TAS
    cytosol GO:0005829 IDA
 Experiment description of studies that identified ECSIT in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for ECSIT
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 APP 351
Two-hybrid Homo sapiens
2 C11orf87  
Affinity Capture-MS Homo sapiens
3 TAS2R19  
Affinity Capture-MS Homo sapiens
4 ITGB1 3688
Affinity Capture-MS Homo sapiens
5 FOXA1  
Affinity Capture-MS Homo sapiens
6 C5AR1  
Affinity Capture-MS Homo sapiens
7 NDUFB8 4714
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 TSPAN15 23555
Affinity Capture-MS Homo sapiens
9 NDUFS1 4719
Affinity Capture-MS Homo sapiens
10 SYNGR1 9145
Affinity Capture-MS Homo sapiens
11 APOD 347
Affinity Capture-MS Homo sapiens
12 TRUB2  
Proximity Label-MS Homo sapiens
13 MTG2  
Proximity Label-MS Homo sapiens
14 NGRN  
Proximity Label-MS Homo sapiens
15 CHCHD1  
Proximity Label-MS Homo sapiens
16 TMEM186  
Affinity Capture-MS Homo sapiens
17 B4GAT1 11041
Affinity Capture-MS Homo sapiens
18 LOXL4 84171
Two-hybrid Homo sapiens
19 ELAVL3  
Two-hybrid Homo sapiens
20 AZGP1 563
Affinity Capture-MS Homo sapiens
21 DNAJC15  
Proximity Label-MS Homo sapiens
22 MTRF1  
Proximity Label-MS Homo sapiens
23 PSEN2 5664
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
24 DECR1 1666
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 CCDC90B  
Proximity Label-MS Homo sapiens
26 LRPPRC 10128
Proximity Label-MS Homo sapiens
27 KIAA1429 25962
Affinity Capture-MS Homo sapiens
28 FBXW4 6468
Two-hybrid Homo sapiens
29 RHEB 6009
Two-hybrid Homo sapiens
30 NDUFS3 4722
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 TFAM 7019
Proximity Label-MS Homo sapiens
32 PMPCA 23203
Proximity Label-MS Homo sapiens
33 HELZ 9931
Affinity Capture-MS Homo sapiens
34 TIMMDC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 GPR88  
Affinity Capture-MS Homo sapiens
36 HINT2 84681
Proximity Label-MS Homo sapiens
37 GCAT  
Affinity Capture-MS Homo sapiens
38 IFIT3 3437
Two-hybrid Homo sapiens
39 GPBAR1  
Affinity Capture-MS Homo sapiens
40 PRDX2 7001
Two-hybrid Homo sapiens
41 DNAJB1 3337
Two-hybrid Homo sapiens
42 MRPS26 64949
Proximity Label-MS Homo sapiens
43 SURF1  
Proximity Label-MS Homo sapiens
44 FAM136A 84908
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 EXD2  
Proximity Label-MS Homo sapiens
46 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 PAXIP1  
Two-hybrid Homo sapiens
48 NDUFAF1  
Co-purification Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 F2RL1  
Affinity Capture-MS Homo sapiens
50 MAVS 57506
Affinity Capture-Western Homo sapiens
51 PMPCB 9512
Proximity Label-MS Homo sapiens
52 TACO1  
Proximity Label-MS Homo sapiens
53 FAF2 23197
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 NDUFS5 4725
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 MTERF3  
Proximity Label-MS Homo sapiens
56 CXCR4 7852
Affinity Capture-MS Homo sapiens
57 FAS 355
Affinity Capture-MS Homo sapiens
58 TRIM59  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
59 MDH2 4191
Proximity Label-MS Homo sapiens
60 APOO 79135
Proximity Label-MS Homo sapiens
61 FARSA 2193
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
62 EXTL3 2137
Affinity Capture-MS Homo sapiens
63 TRMT61B  
Affinity Capture-MS Homo sapiens
64 ATAD3B 83858
Affinity Capture-MS Homo sapiens
65 C6orf203  
Proximity Label-MS Homo sapiens
66 NDUFC2 4718
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 COX15 1355
Proximity Label-MS Homo sapiens
68 CEP55 55165
Two-hybrid Homo sapiens
69 FASTKD5  
Proximity Label-MS Homo sapiens
70 RNF32  
Two-hybrid Homo sapiens
71 IFIT5 24138
Two-hybrid Homo sapiens
72 CLPP 8192
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 ACADVL 37
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 NDUFA8 4702
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 A2M 2
Two-hybrid Homo sapiens
76 TUFM 7284
Proximity Label-MS Homo sapiens
77 LRRC25  
Affinity Capture-MS Homo sapiens
78 KDSR 2531
Affinity Capture-MS Homo sapiens
79 HSCB 150274
Proximity Label-MS Homo sapiens
80 GEM  
Two-hybrid Homo sapiens
81 NDUFS8 4728
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 PARP16 54956
Affinity Capture-MS Homo sapiens
83 TSSK3  
Two-hybrid Homo sapiens
84 ACTN4 81
Co-fractionation Homo sapiens
85 OGT 8473
Reconstituted Complex Homo sapiens
86 CCDC109B 55013
Proximity Label-MS Homo sapiens
87 EXOC6 54536
Two-hybrid Homo sapiens
88 TBRG4 9238
Proximity Label-MS Homo sapiens
89 CYP24A1  
Affinity Capture-MS Homo sapiens
90 IPPK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 SOWAHA  
Affinity Capture-MS Homo sapiens
92 CTNNA3  
Two-hybrid Homo sapiens
93 MRPL11 65003
Proximity Label-MS Homo sapiens
94 MTRF1L  
Proximity Label-MS Homo sapiens
95 DNAJC30  
Proximity Label-MS Homo sapiens
96 COA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 TMEM70  
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 NDUFAF3 25915
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 CD93  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 COX4I1 1327
Proximity Label-MS Homo sapiens
101 ND1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
102 NDUFB5 4711
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 CLEC5A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 CYP26B1  
Affinity Capture-MS Homo sapiens
105 ACTN1 87
Co-fractionation Homo sapiens
106 MARVELD2 153562
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
107 VIPR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
108 SETDB1  
Two-hybrid Homo sapiens
109 PPP2R2B 5521
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
110 PSEN1 5663
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
111 MRRF  
Proximity Label-MS Homo sapiens
112 TMEM126B 55863
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
113 MTIF2 4528
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
114 MCCC2 64087
Affinity Capture-MS Homo sapiens
115 NDUFA13 51079
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
116 APOE 348
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
117 SLC25A51  
Proximity Label-MS Homo sapiens
118 RAD23A 5886
Two-hybrid Homo sapiens
119 RELA 5970
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
120 ZDHHC12  
Affinity Capture-MS Homo sapiens
121 ATP6V1C2 245973
Affinity Capture-MS Homo sapiens
122 GCDH 2639
Two-hybrid Homo sapiens
123 SOD3 6649
Affinity Capture-MS Homo sapiens
124 CMKLR1 1240
Affinity Capture-MS Homo sapiens
125 TARSL2 123283
Two-hybrid Homo sapiens
126 TRAF6 7189
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
127 GPR12  
Affinity Capture-MS Homo sapiens
128 RMND1 55005
Proximity Label-MS Homo sapiens
129 BFSP2  
Two-hybrid Homo sapiens
130 ABCC10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
131 PI4K2A 55361
Two-hybrid Homo sapiens
132 MAP3K7  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
133 LPAR1 1902
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
134 FBXL12 54850
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
135 KIAA0430  
Affinity Capture-MS Homo sapiens
136 C17orf80 55028
Proximity Label-MS Homo sapiens
137 MRPS17 51373
Affinity Capture-MS Homo sapiens
138 CYP2C18  
Two-hybrid Homo sapiens
139 C4A 720
Affinity Capture-MS Homo sapiens
140 FAM134C 162427
Affinity Capture-MS Homo sapiens
141 MYC  
Affinity Capture-MS Homo sapiens
142 NDUFA2 4695
Affinity Capture-MS Homo sapiens
143 ACKR2  
Affinity Capture-MS Homo sapiens
144 BLMH 642
Two-hybrid Homo sapiens
145 ASPH 444
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
146 AP1M2 10053
Two-hybrid Homo sapiens
147 NFKB1 4790
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
148 ACAD9 28976
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
149 ABCB10 23456
Co-fractionation Homo sapiens
150 NDUFA3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
151 CYP2C9  
Two-hybrid Homo sapiens
152 RAB3A 5864
Two-hybrid Homo sapiens
153 S1PR1 1901
Affinity Capture-MS Homo sapiens
154 STAMBPL1  
Two-hybrid Homo sapiens
155 TLR4  
Phenotypic Suppression Homo sapiens
156 FFAR1  
Affinity Capture-MS Homo sapiens
157 NDUFB11 54539
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
158 MCUR1 63933
Proximity Label-MS Homo sapiens
159 NDUFS2 4720
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
160 P2RY12 64805
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
161 IMMT 10989
Two-hybrid Homo sapiens
162 TMEM9 252839
Affinity Capture-MS Homo sapiens
163 CYP2C8  
Two-hybrid Homo sapiens
164 DHX15 1665
Co-fractionation Homo sapiens
165 CS 1431
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
166 LLGL2 3993
Affinity Capture-MS Homo sapiens
167 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
168 TSFM 10102
Proximity Label-MS Homo sapiens
169 AUH 549
Proximity Label-MS Homo sapiens
170 NXF1 10482
Two-hybrid Homo sapiens
171 DNAJC19 131118
Proximity Label-MS Homo sapiens
172 SIDT2 51092
Affinity Capture-MS Homo sapiens
173 C3AR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
174 ICT1 3396
Proximity Label-MS Homo sapiens
175 STRN4 29888
Two-hybrid Homo sapiens
176 APEX1 328
Affinity Capture-RNA Homo sapiens
177 CDC37 11140
Two-hybrid Homo sapiens
178 PDHA1 5160
Proximity Label-MS Homo sapiens
179 DNAJA3 9093
Proximity Label-MS Homo sapiens
180 SMURF1 57154
Co-localization Homo sapiens
181 SQSTM1 8878
Affinity Capture-Western Homo sapiens
182 MKRN3  
Two-hybrid Homo sapiens
183 NDUFS7 374291
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
184 NDUFB1 4707
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
185 EXOSC1 51013
Two-hybrid Homo sapiens
186 C21orf33  
Proximity Label-MS Homo sapiens
187 SSBP1 6742
Proximity Label-MS Homo sapiens
188 NNT 23530
Affinity Capture-MS Homo sapiens
189 LPCAT2 54947
Affinity Capture-MS Homo sapiens
190 LONP1 9361
Two-hybrid Homo sapiens
191 GFM1 85476
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
192 AARS2  
Proximity Label-MS Homo sapiens
193 TMEM79  
Affinity Capture-MS Homo sapiens
194 MGME1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens